Flna (NM_010227) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ABP-280; Dilp2; F730004A14Rik; filamin-1; Fln1; GENA 379
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR227403 representing NM_010227 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCTCTCACTCCCGCTGTGGCCAGAGTGCGGCGGTTGCGTCTCCGGGAGGCAGTATCGATTCAC GGGACGCGGAGATGCCGGCTACCGAAAAAGACCTAGCAGAAGATGCACCGTGGAAGAAAATTCAGCAGAA CACATTCACCCGCTGGTGCAATGAGCACCTTAAGTGCGTAAGCAAGCGCATCGCCAATCTGCAGACGGAC CTGAGCGATGGGTTGCGGCTCATCGCGCTGCTCGAGGTACTCAGCCAGAAGAAAATGCACCGCAAGCACA ACCAAAGACCCACTTTCCGCCAGATGCAGCTCGAAAATGTGTCGGTGGCGCTTGAATTCCTGGACCGTGA GAGCATCAAGCTCGTGTCCATAGACAGCAAGGCTATTGTGGATGGAAATCTGAAGCTGATCTTAGGCCTC ATCTGGACCCTGATCCTGCACTATTCCATCTCAATGCCCATGTGGGATGAGGAAGAGGATGAGGAGGCCA AGAAGCAAACACCCAAGCAGAGGCTTCTAGGCTGGATTCAGAACAAGCTACCACAGCTTCCCATTACCAA CTTCAGTCGAGACTGGCAGAGTGGCCGGGCCCTGGGTGCTCTTGTTGATAGCTGTGCCCCAGGCCTATGT CCTGACTGGGACTCCTGGGATGCTAGTAAGCCTGTGAACAATGCACGGGAAGCCATGCAGCAGGCTGATG ACTGGCTAGGCATTCCTCAGGTGATTACCCCAGAAGAAATTGTGGACCCCAATGTAGATGAGCATTCTGT TATGACCTACCTGTCTCAGTTTCCCAAGGCCAAGCTGAAGCCAGGGGCTCCTCTTCGGCCCAAACTGAAC CCGAAGAAAGCCCGAGCCTATGGGCCAGGCATCGAGCCTACAGGCAATATGGTGAAGAAGAGAGCAGAAT TCACTGTGGAGACCCGAAGTGCTGGACAGGGAGAAGTGCTTGTATATGTGGAGGACCCAGCTGGACACCA GGAAGAGGCAAAAGTGACTGCCAATAATGACAAGAACCGTACTTTCTCTGTCTGGTATGTCCCTGAAGTG ACAGGGACTCATAAGGTGACTGTGCTCTTTGCTGGCCAACATATTGCCAAGAGCCCCTTTGAGGTGTATG TGGACAAGTCACAGGGTGATGCCAGCAAAGTGACTGCCCAGGGCCCTGGTCTGGAGCCCAGTGGCAATAT TGCCAACAAGACTACCTACTTTGAGATCTTCACTGCAGGAGCTGGCATGGGTGAGGTGGAAGTTGTCATC CAGGACCCTACAGGACAGAAAGGCACAGTGGAACCTCAGCTGGAGGCCAGGGGTGACAGCACCTATCGCT GTAGCTATCAGCCCACCATGGAGGGTGTCCATACAGTACATGTCACCTTCGCCGGTGTTCCCATCCCTCG TAGCCCCTACACTGTCACTGTTGGCCAAGCCTGTAACCCAGCTGCCTGCCGGGCTATTGGTAGAGGCCTT CAGCCCAAGGGTGTTCGAGTGAAGGAAACAGCCGACTTCAAGGTGTACACAAAGGGCGCTGGCAGTGGGG AGCTAAAGGTCACTGTAAAGGGTCCCAAGGGTGAGGAGCGTGTAAAGCAGAAGGACTTAGGGGATGGTGT GTATGGCTTTGAATATTACCCTACAATCCCTGGCACATACACTGTCACCATCACATGGGGTGGCCAGAAC ATTGGTCGAAGTCCGTTTGAGGTGAAGGTAGGCACTGAGTGTGGCAATCAGAAAGTTCGGGCATGGGGTC CTGGCCTGGAAGGAGGCATTGTTGGCAAGTCAGCAGACTTCGTAGTAGAGGCCATTGGTGATGATGTGGG CACCTTGGGTTTCTCTGTGGAAGGTCCATCACAGGCAAAGATTGAATGTGACGACAAGGGTGATGGCTCC TGTGATGTGCGCTATTGGCCCCAGGAGGCTGGCGAGTATGCTGTTCATGTGCTGTGTAACAGTGAGGATA TCCGTCTCAGTCCTTTCATGGCTGACATCCGTGAGGCACCCCAGGATTTTCACCCAGACAGGGTGAAGGC ACGTGGGCCTGGATTGGAGAAGACTGGTGTGGCTGTCAACAAGCCAGCAGAGTTCACAGTTGATGCCAAG CATGCTGGGAAGGCTCCTCTTCGAGTTCAAGTTCAGGACAATGAGGGCTGCTCTGTGGAAGCGACAGTCA AGGACAATGGCAATGGTACTTACAGCTGTTCTTATGTGCCCAGAAAGCCAGTGAAGCACACAGCCATGGT TTCTTGGGGAGGTGTCAGCATCCCCAACAGTCCTTTCCGGGTGAATGTGGGAGCTGGCAGCCATCCAAAC AAAGTCAAGGTGTATGGTCCAGGAGTGGCCAAGACTGGGCTCAAGGCCCATGAACCTACCTACTTTACTG TGGATTGTACTGAAGCTGGCCAGGGAGATGTCAGCATTGGTATCAAGTGTGCCCCTGGAGTAGTGGGCCC CACTGAGGCTGATATTGACTTTGATATCATCCGCAATGACAATGACACCTTCACTGTAAAATACACACCC TGTGGGGCTGGCAGCTATACCATCATGGTCCTTTTTGCTGACCAGGCCACACCCACCAGCCCCATCAGAG TCAAAGTGGAGCCTTCTCATGATGCCAGCAAGGTGAAGGCTGAGGGTCCTGGCCTAAATCGCACTGGTGT TGAACTTGGCAAACCCACCCATTTCACAGTCAATGCTAAAACTGCTGGGAAAGGCAAGCTGGATGTCCAA TTCTCAGGACTGGCTAAGGGAGATGCAGTACGGGATGTGGACATCATTGACCACCATGATAATACCTACA CAGTCAAGTACATTCCTGTGCAGCAGGGCCCAGTAGGTGTCAATGTCACTTATGGAGGAGATCACATCCC CAAGAGTCCATTTTCAGTGGGAGTATCTCCAAGCCTGGATCTCAGCAAAATCAAGGTGTCTGGCCTTGGT GACAAAGTGGACGTTGGCAAAGATCAAGAGTTCACAGTAAAGTCAAAGGGTGCAGGTGGTCAAGGCAAAG TAGCATCCAAGATTGTGAGTCCCTCAGGTGCAGCGGTACCCTGCAAGGTAGAGCCAGGCCTGGGAGCTGA CAACAGCGTGGTACGTTTTGTGCCCCGTGAAGAGGGGCCCTATGAGGTGGAAGTGACCTATGATGGTGTG CCTGTACCTGGCAGTCCCTTTCCACTAGAAGCTGTGGCCCCCACCAAACCCAGCAAGGTGAAGGCGTTTG GACCAGGGCTACAGGGGGGCAATGCAGGCTCCCCTGCCCGCTTCACCATTGATACAAAGGGTGCTGGCAC TGGTGGCCTGGGCCTGACAGTGGAAGGCCCCTGTGAAGCACAGCTTGAGTGCCTAGACAACGGGGATGGT ACATGCTCTGTGTCTTATGTACCCACTGAGCCTGGGGACTACAACATCAACATCCTTTTTGCTGACACCC ACATTCCTGGATCCCCATTCAAGGCCCATGTGGCTCCTTGTTTTGATGCATCCAAGGTGAAGTGCTCAGG CCCTGGGCTGGAGCGGGCTACTGCTGGTGAGGTAGGGCAGTTCCAAGTGGACTGTTCAAGTGCTGGCAGT GCTGAGTTGACGATTGAGATCTGCTCTGAGGCAGGACTGCCAGCTGAAGTATACATTCAAGACCATGGTG ATGGCACACACACCATTACCTATATTCCTCTCTGTCCTGGGGCTTACACTGTTACCATCAAGTATGGCGG CCAGCCTGTGCCCAACTTCCCCAGCAAGCTACAGGTGGAACCTGCTGTAGATACCTCAGGTGTACAGTGC TATGGGCCTGGGATTGAAGGTCAAGGTGTCTTCCGAGAGGCAACCACTGAGTTCAGTGTGGATGCCCGGG CTCTTACACAGACTGGAGGGCCACATGTCAAGGCTCGAGTGGCCAACCCCTCAGGCAATCTGACAGATAC CTATGTGCAAGACTGTGGGGATGGCACATACAAAGTGGAATACACTCCATATGAGGAAGGAGTACACTCT GTGGATGTGACTTATGATGGCAGCCCTGTGCCCAGCAGCCCCTTCCAGGTGCCTGTAACAGAGGGCTGTG ACCCCTCCCGGGTGCGTGTCCATGGACCAGGCATCCAAAGTGGTACCACCAACAAACCCAACAAGTTCAC AGTAGAAACCAGGGGAGCTGGCACAGGTGGCCTGGGCTTGGCTGTTGAGGGTCCCTCAGAGGCCAAGATG TCTTGTATGGATAATAAAGATGGCAGCTGCTCAGTAGAATACATCCCCTATGAAGCTGGAACCTATAGCC TTAATGTCACTTATGGTGGTCACCAAGTGCCAGGTAGTCCCTTCAAGGTCCCTGTACATGATGTGACAGA TGCATCTAAAGTCAAGTGTTCTGGACCTGGCCTAAGCCCAGGCATGGTCCGTGCCAACCTCCCTCAGTCC TTTCAGGTGGACACAAGCAAAGCTGGAGTTGCCCCACTGCAGGTCAAAGTGCAGGGGCCCAAAGGCCTGG TGGAGCCAGTGGATGTAGTGGACAATGCTGATGGTACTCAGACTGTCAACTATGTGCCCAGCCGAGAAGG GTCCTATAGCATTTCTGTGCTGTATGGTGAAGAAGAAGTGCCACGGAGCCCCTTCAAGGTCAAGGTGCTG CCTACACATGATGCCAGTAAGGTGAAGGCCAGTGGACCTGGACTCAACACCACTGGTGTACCTGCTAGCC TGCCTGTGGAGTTCACCATTGATGCCAAGGATGCTGGGGAGGGTCTGTTGGCTGTCCAGATTACGGATCC TGAAGGCAAGCCCAAGAAGACACACATTCAAGATAATCATGATGGCACATACACGGTGGCTTATGTGCCA GATGTGCCAGGCCGGTACACAATCCTCATCAAGTATGGTGGTGATGAGATTCCCTTTTCCCCGTACCGTG TCCGGGCTGTGCCCACTGGGGATGCCAGCAAGTGCACAGTCACAGGTGCTGGCATTGGCCCCACCATCCA GATTGGGGAGGAGACGGTGATTACTGTGGACACAAAAGCAGCAGGCAAAGGCAAAGTGACTTGTACTGTG TGCACACCTGATGGCTCAGAGGTAGACGTGGACGTGGTGGAGAATGAGGATGGCACCTTTGACATCTTCT ACACAGCTCCCCAACCGGGCAAATATGTCATCTGTGTGCGCTTCGGTGGCGAGCATGTGCCCAACAGCCC CTTCCAAGTTACAGCTTTGGCTGGGGACCAACCAACAGTGCAGACCCCATTAAGGTCTCAGCAGCTGGCT CCACAGTATAACTATCCTCAGGGTAGCCAGCAAACCTGGATTCCAGAGAGGCCCATGGTGGGCGTTAATG GGCTGGATGTGACCAGCCTGAGGCCCTTTGATCTTGTCATCCCCTTCACTATCAAGAAGGGGGAGATCAC TGGGGAAGTTCGAATGCCCTCAGGCAAGGTGGCCCAGCCTTCCATTACTGATAACAAAGATGGCACTGTT ACTGTACGTTACTCACCCAGTGAAGCTGGCCTGCATGAAATGGACATTCGCTATGACAATATGCATATCC CAGGAAGCCCTCTGCAGTTCTATGTTGATTATGTCAACTGTGGCCACATCACTGCTTATGGTCCTGGCCT TACCCATGGAGTGGTCAACAAACCTGCCACCTTCACTGTCAACACCAAGGATGCAGGAGAGGGGGGCTTG TCTCTGGCCATTGAGGGTCCATCTAAAGCAGAAATCAGTTGCACTGACAACCAGGATGGAACATGCAGTG TCTCTTACCTGCCTGTACTGCCTGGTGACTATAGCATCCTAGTTAAGTACAATGATCAACACATCCCAGG CAGTCCCTTTACTGCCAGAGTAACAGGTGACGATTCCATGCGTATGTCCCACCTAAAGGTGGGTTCTGCT GCTGATATCCCCATCAATATCTCAGAAACAGACCTTAGCCTACTCACAGCCACTGTGGTGCCACCTTCGG GTCGAGAGGAACCCTGTCTGCTGAAACGTTTGCGAAATGGCCACGTGGGGATTTCCTTCGTGCCCAAGGA GACAGGGGAGCACCTGGTACATGTGAAGAAGAATGGCCAGCATGTGGCAAGCAGTCCCATCCCAGTAGTG ATCAGCCAGTCGGAGATAGGTGATGCCAGCCGTGTGAGGGTCTCTGGTCAAGGTCTTCATGAAGGTCATA CCTTTGAGCCTGCAGAGTTTATTATTGACACCAGAGATGCAGGCTACGGTGGGCTTAGTCTGTCCATTGA GGGCCCTAGCAAAGTAGACATCAACACAGAGGATCTGGAGGATGGCACATGCAGGGTCACCTACTGTCCC ACAGAGCCTGGAAACTACATTATAAACATCAAATTTGCTGACCAGCATGTGCCTGGCAGTCCCTTTTCTG TGAAGGTGACAGGTGAGGGCCGGGTGAAAGAGAGTATCACACGCAGGCGACGTGCCCCTTCTGTGGCCAA TATTGGCAGTCATTGTGACCTCAGCCTGAAGATTCCTGAAATTAGCATCCAAGATATGACAGCCCAGGTG ACCAGCCCATCAGGCAAGACCCATGAGGCAGAGATCGTAGAAGGAGAGAACCATACTTACTGTATCCGAT TTGTGCCTGCTGAGATGGGAATGCATACAGTCAGTGTCAAGTACAAGGGCCAGCATGTACCTGGGAGCCC CTTCCAGTTCACTGTGGGGCCTCTGGGGGAAGGGGGTGCTCACAAGGTCCGTGCTGGAGGCCCTGGCCTA GAGAGGGCTGAAGTTGGAGTGCCAGCGGAGTTCGGCATTTGGACTAGGGAAGCTGGCGCTGGAGGCCTGG CCATTGCTGTTGAAGGCCCCAGCAAGGCTGAGATCTCTTTCGAGGACCGAAAGGATGGCTCCTGTGGTGT GGCCTACGTAGTTCAGGAGCCAGGTGACTATGAGGTCTCAGTCAAGTTCAACGAGGAGCACATACCTGAT AGCCCCTTCGTGGTGCCTGTGGCTTCTCCGTCTGGTGACGCCCGCCGCCTTACTGTTTCTAGTCTTCAGG AGTCAGGGTTAAAGGTCAACCAGCCAGCATCTTTTGCAGTCAGTCTGAATGGAGCCAAGGGGGCAATTGA TGCCAAGGTGCACAGCCCCTCAGGAGCTCTGGAGGAGTGCTATGTCACAGAGATTGACCAAGATAAGTAT GCTGTGCGTTTCATCCCACGAGAGAATGGCATCTACTTGATTGATGTCAAGTTCAATGGTACTCACATTC CTGGAAGTCCCTTCAAGATCCGAGTTGGGGAGCCTGGGCATGGAGGGGACCCAGGCTTAGTGTCCGCCTA TGGAGCAGGCCTGGAAGGTGGTGTCACAGGGAGCCCAGCAGAGTTTATTGTGAACACAAGCAATGCAGGA GCTGGTGCCCTTTCGGTTACCATTGATGGCCCCTCCAAGGTGAAGATGGATTGCCAGGAGTGCCCCGAGG GCTATCGTGTCACCTATACCCCCATGGCACCTGGCAGCTACCTCATCTCCATCAAGTATGGTGGCCCCTA TCACATTGGTGGAAGTCCCTTTAAAGCCAAGGTCACAGGTCCTCGTCTTGTAAGCAACCACAGCCTCCAT GAGACATCATCTGTGTTTGTGGACTCTTTGACTAAAGTTGCCACGGTTCCCCAGCATGCAACCTCAGGCC CAGGTCCTGCTGATGTCAGCAAGGTAGTAGCCAAAGGCCTGGGGCTAAGCAAGGCTTATGTAGGCCAGAA GAGCAACTTCACAGTAGATTGCAGCAAAGCAGGTAACAACATGCTGCTGGTGGGCGTGCATGGCCCAAGG ACACCCTGTGAAGAGATCCTGGTGAAACACATGGGCAGCCGCCTCTATAGTGTCTCTTACCTGCTCAAGG ACAAAGGGGAGTACACACTGGTGGTCAAGTGGGGTGATGAGCATATCCCAGGCAGCCCATACCGCATTAT GGTACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR227403 representing NM_010227 Red=Cloning site Green=Tags(s) MSSSHSRCGQSAAVASPGGSIDSRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVSKRIANLQTD LSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGL IWTLILHYSISMPMWDEEEDEEAKKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLC PDWDSWDASKPVNNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLN PKKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEV TGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANKTTYFEIFTAGAGMGEVEVVI QDPTGQKGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPAACRAIGRGL QPKGVRVKETADFKVYTKGAGSGELKVTVKGPKGEERVKQKDLGDGVYGFEYYPTIPGTYTVTITWGGQN IGRSPFEVKVGTECGNQKVRAWGPGLEGGIVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGS CDVRYWPQEAGEYAVHVLCNSEDIRLSPFMADIREAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAK HAGKAPLRVQVQDNEGCSVEATVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVSIPNSPFRVNVGAGSHPN KVKVYGPGVAKTGLKAHEPTYFTVDCTEAGQGDVSIGIKCAPGVVGPTEADIDFDIIRNDNDTFTVKYTP CGAGSYTIMVLFADQATPTSPIRVKVEPSHDASKVKAEGPGLNRTGVELGKPTHFTVNAKTAGKGKLDVQ FSGLAKGDAVRDVDIIDHHDNTYTVKYIPVQQGPVGVNVTYGGDHIPKSPFSVGVSPSLDLSKIKVSGLG DKVDVGKDQEFTVKSKGAGGQGKVASKIVSPSGAAVPCKVEPGLGADNSVVRFVPREEGPYEVEVTYDGV PVPGSPFPLEAVAPTKPSKVKAFGPGLQGGNAGSPARFTIDTKGAGTGGLGLTVEGPCEAQLECLDNGDG TCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVAPCFDASKVKCSGPGLERATAGEVGQFQVDCSSAGS AELTIEICSEAGLPAEVYIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQC YGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANPSGNLTDTYVQDCGDGTYKVEYTPYEEGVHS VDVTYDGSPVPSSPFQVPVTEGCDPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKM SCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQS FQVDTSKAGVAPLQVKVQGPKGLVEPVDVVDNADGTQTVNYVPSREGSYSISVLYGEEEVPRSPFKVKVL PTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNHDGTYTVAYVP DVPGRYTILIKYGGDEIPFSPYRVRAVPTGDASKCTVTGAGIGPTIQIGEETVITVDTKAAGKGKVTCTV CTPDGSEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHVPNSPFQVTALAGDQPTVQTPLRSQQLA PQYNYPQGSQQTWIPERPMVGVNGLDVTSLRPFDLVIPFTIKKGEITGEVRMPSGKVAQPSITDNKDGTV TVRYSPSEAGLHEMDIRYDNMHIPGSPLQFYVDYVNCGHITAYGPGLTHGVVNKPATFTVNTKDAGEGGL SLAIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNDQHIPGSPFTARVTGDDSMRMSHLKVGSA ADIPINISETDLSLLTATVVPPSGREEPCLLKRLRNGHVGISFVPKETGEHLVHVKKNGQHVASSPIPVV ISQSEIGDASRVRVSGQGLHEGHTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDLEDGTCRVTYCP TEPGNYIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANIGSHCDLSLKIPEISIQDMTAQV TSPSGKTHEAEIVEGENHTYCIRFVPAEMGMHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGL ERAEVGVPAEFGIWTREAGAGGLAIAVEGPSKAEISFEDRKDGSCGVAYVVQEPGDYEVSVKFNEEHIPD SPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKY AVRFIPRENGIYLIDVKFNGTHIPGSPFKIRVGEPGHGGDPGLVSAYGAGLEGGVTGSPAEFIVNTSNAG AGALSVTIDGPSKVKMDCQECPEGYRVTYTPMAPGSYLISIKYGGPYHIGGSPFKAKVTGPRLVSNHSLH ETSSVFVDSLTKVATVPQHATSGPGPADVSKVVAKGLGLSKAYVGQKSNFTVDCSKAGNNMLLVGVHGPR TPCEEILVKHMGSRLYSVSYLLKDKGEYTLVVKWGDEHIPGSPYRIMVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_010227
ORF Size	7917 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010227.3, NP_034357.2
RefSeq Size	8347 bp
RefSeq ORF	7920 bp
Locus ID	192176
MW	280.5 kDa
Gene Summary	Actin binding protein that promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins (By similarity). Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Plays a role in cell-cell contacts and adherens junctions during the development of blood vessels, heart and brain organs (PubMed:17172441). Plays a role in platelets morphology through interaction with SYK that regulates ITAM- and ITAM-like-containing receptor signaling, resulting in by platelet cytoskeleton organization maintenance (PubMed:20713593).[UniProtKB/Swiss-Prot Function]

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