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Slit2 (NM_178804) Mouse Tagged ORF Clone
CAT＃: MR227608
- TrueORF®
Slit2 (Myc-DDK-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2)
ORF Plasmid: tGFP
"NM_178804" in other vectors (4)
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CNY 15,608.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | b2b1200.1Clo; Drad; Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; S; Slil3; slit-2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR227608 representing NM_178804
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCGCTGGGGTTAGTGTTGTCGATCTTGAACAAGGTGG CGCCGCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACGGTGGACTGTCATGGGCTGGCACTGCG CAGTGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTGAATGGAAATAACATCACGAGGATC ACGAAGATAGATTTTGCTGGTCTCAGGCACCTCAGAGTTCTTCAGCTCATGGAGAACAGAATCAGCACCA TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTGGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT GTTTCCTGAGCTGCTGTTTCTCGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAAATTCAA GCAATTCCAAGGAAGGCTTTCCGTGGGGCAGTTGACATTAAAAACCTGCAACTGGATTACAACCAGATCA GCTGCATTGAAGATGGGGCGTTCAGAGCTCTACGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCGAACAAC TTGTACTGCGACTGCCACCTAGCCTGGCTCTCAGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCACAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG CAGTGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCCGCTGCTTGTACCTGTAGC AACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTGAGACCATCACAG AAATACGTTTGGAACAGAACTCCATCAGGGTCATCCCTCCAGGAGCCTTCTCACCATACAAAAAGCTTAG ACGACTAGACCTGAGCAACAACCAGATCTCTGAACTTGCACCAGATGCCTTCCAAGGACTGCGCTCTCTG AATTCACTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTCGAAGGACTATTTTCCT TGCAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCAGGACCTGCACAA CTTGAACCTTCTCTCCTTATATGACAATAAGCTTCAGACGGTTGCCAAGGGCACCTTCTCAGCCCTCAGA GCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGTGGCTAGCGGATT ATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGCCCCCGCCGCCTGGCAAACAAAAG AATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGGTACAGAAGATTATCGATCAAAATTAAGTGGA GACTGCTTTGCAGACTTGGCTTGTCCTGAGAAGTGTCGCTGTGAAGGGACCACAGTAGACTGCTCCAATC AAAGACTCAACAAAATCCCTGACCATATTCCCCAGTACACAGCAGAGCTGCGTCTCAATAATAATGAATT CACAGTGTTAGAAGCCACGGGAATATTTAAGAAACTTCCTCAGTTACGTAAAATCAACTTTAGCAACAAT AAGATCACGGATATCGAGGAGGGTGCATTTGAAGGCGCGTCTGGTGTGAATGAAATTCTTCTCACCAGTA ACCGTTTGGAAAATGTTCAGCATAAGATGTTCAAAGGACTGGAGAGCCTCAAAACATTGATGCTGAGAAG TAATCGAATAAGCTGTGTTGGGAACGACAGTTTCATAGGACTCGGCTCTGTGCGTCTGCTCTCTTTATAT GACAATCAAATTACCACAGTGGCACCAGGAGCATTTGATTCTCTCCATTCATTATCCACTCTAAACCTCT TGGCCAATCCTTTCAACTGTAACTGTCACCTGGCATGGCTGGGAGAATGGCTCAGAAGGAAAAGAATTGT AACAGGAAATCCTCGATGCCAAAAACCCTACTTCCTGAAGGAAATCCCAATCCAGGATGTAGCCATTCAG GACTTCACCTGTGATGATGGAAATGATGACAATAGTTGCTCTCCACTCTCCCGTTGTCCTTCTGAATGTA CCTGCTTGGATACAGTGGTACGATGTAGCAACAAGGGCTTGAAGGTTTTGCCTAAAGGTATTCCAAAAGA TGTCACAGAGCTGTATCTGGATGGGAACCAGTTTACGCTGGTCCCGAAGGAACTCTCTAACTACAAACAT TTAACACTTATAGACTTAAGTAACAACCGAATAAGCACCCTTTCCAATCAAAGCTTCAGCAACATGACCC AGCTTCTCACCTTAATCCTCAGTTACAACCGTCTGAGATGTATCCCTCCACGAACCTTTGATGGATTGAA GTCTCTTCGGTTACTGTCTTTACATGGAAATGACATTTCTGTTGTGCCTGAAGGTGCCTTCAATGACTTG TCAGCCTTGTCACACTTAGCGATTGGAGCCAACCCTCTTTACTGTGATTGTAACATGCAGTGGTTATCCG ACTGGGTGAAGTCGGAATATAAGGAACCTGGAATTGCACGCTGTGCCGGCCCTGGAGAAATGGCAGATAA ATTATTACTCACTACTCCCTCCAAAAAATTTACATGTCAAGGTCCCGTGGATATCACTATTCAAGCCAAG TGTAATCCCTGCTTATCAAATCCATGTAAAAATGATGGCACCTGTAACAATGACCCCGTTGATTTTTATC GATGTACCTGCCCATATGGATTCAAGGGTCAGGACTGTGATGTCCCCATTCATGCTTGTATCAGTAATCC ATGTAAACATGGAGGAACTTGTCACTTAAAGGAAGGAGAGAATGCTGGATTCTGGTGCACTTGTGCTGAT GGGTTTGAAGGAGAAAACTGTGAAGTCAATATTGATGATTGTGAAGATAATGATTGTGAAAATAATTCTA CATGCGTTGATGGAATTAACAACTACACATGTCTTTGCCCACCGGAATACACAGGTGAACTGTGTGAGGA AAAGCTGGACTTCTGTGCACAAGACTTGAATCCCTGCCAGCATGACTCCAAGTGCATCCTGACTCCAAAG GGATTCAAGTGTGACTGCACTCCAGGATACATTGGTGAGCACTGTGACATTGACTTTGATGACTGCCAAG ATAACAAGTGTAAAAACGGTGCTCACTGCACAGATGCCGTGAACGGATACACGTGCGTCTGTCCTGAAGG CTACAGTGGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCCTGTGATAATTTT GATTGCCAGAATGGAGCCCAGTGTATCATCAGGATAAATGAACCAATATGCCAGTGTTTGCCTGGCTACC TGGGAGAGAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCTTCAGATTCCTTC AGCCAAGGTTCGGCCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGCGGCATCCTCTTG TATAAAGGTGACAAAGACCACATTGCCGTGGAACTCTATAGAGGGCGAGTTCGAGCCAGCTATGACACCG GCTCTCATCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAGCT ACTGACCCTGGATTCCAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAGTCATCACCAATTTGTCA AAACAATCTACTCTGAATTTCGACTCTCCACTCTATGTAGGAGGCATGCCTGGGAAAAATAACGTGGCAT CCCTGCGCCAGGCCCCTGGGCAAAATGGCACCAGCTTCCATGGCTGTATCCGGAACCTTTACATTAACAG TGAGCTGCAGGACTTCCGGAAAATGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAACCATGCCACAAG AAAGTATGTGCCCATGGCATGTGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAATGTGAGGAAGGGT GGATGGGGCCCCTCTGTGACCAGAGAACCAATGATCCCTGCCTCGGAAACAAATGTGTGCATGGGACCTG CCTGCCCATCAATGCCTTCTCCTATAGTTGCAAGTGCCTGGAGGGCCATGGCGGTGTCCTCTGTGATGAA GAAGAAGATCTCTTTAACCCCTGCCAGATGATCAAGTGCAAGCATGGGAAGTGCAGGCTTTCTGGAGTGG GCCAGCCCTATTGTGAATGCAACAGTGGATTCACCGGGGACAGCTGTGATAGAGAAATTTCTTGTCGAGG GGAACGGATAAGGGACTATTACCAGAAGCAGCAGGGTTACGCTGCCTGTCAAACAACTAAGAAAGTATCT CGCTTGGAATGCAGAGGCGGGTGCGCTGGAGGCCAGTGCTGTGGACCTCTGAGAAGCAAGAGGCGGAAAT ACTCTTTCGAATGCACAGATGGCTCCTCATTTGTGGACGAGGTTGAGAAAGTGGTGAAGTGCGGCTGCGC GAGATGTGCCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR227608 representing NM_178804
Red=Cloning site Green=Tags(s) MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRI TKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQ AIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNN LYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAACTCS NNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSL NSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALR AIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSG DCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNN KITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLY DNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQ DFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQFTLVPKELSNYKH LTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDL SALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAK CNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCAD GFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTPK GFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNF DCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILL YKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLS KQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTGILPGCEPCHK KVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLPINAFSYSCKCLEGHGGVLCDE EEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGERIRDYYQKQQGYAACQTTKKVS RLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARCAS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178804 |
| ORF Size | 4563 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178804.5, NP_848919.3 |
| RefSeq Size | 7988 bp |
| RefSeq ORF | 4566 bp |
| Locus ID | 20563 |
| UniProt ID | Q9R1B9 |
| MW | 169.2 kDa |
| Gene Summary | The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224640 | Slit2 (untagged) - Mouse slit homolog 2 (Drosophila) (Slit2), (10ug) |
CNY 19,570.00 |
|
| MG227608 | Slit2 (tGFP-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2), (10ug) |
CNY 17,208.00 |
|
| MR227608L3 | Lenti ORF clone of Slit2 (Myc-DDK-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2) |
CNY 17,100.00 |
|
| MR227608L4 | Lenti ORF clone of Slit2 (mGFP-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2) |
CNY 18,008.00 |
