Vps25 (NM_001284412) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110020N13Rik; AW107467; D11Wsu68e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228270 representing NM_001284412 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATGAGTTTCGAGTGGCCGTGGCAGTACCGCTTCCCGCCCTTCTTTACGTTACAGCCGAACGTGG ACACCCGGCAGAAGCAGCTGGCCGCCTGGTGCTCTCTGGTTCTGTCCTTCTGCCGCCTGCACAAACAGTC CAGCATGACGGTGATGGAAGCCCAGGAGAGCCCGCTTTTCAACAACGTCAAGCTACAGCGGAAACTTCCT GTGGAGTCAATTCAGATTGTATTAGAAGAACTGAGAAAGAAAGGGAACCTCGAGTGGTTGGATAAGAATA AGTCTAGCTTCCTGATCATGTGGCGGAGGCCAGAAGAATGGGGGAAACTCATTTACCAATGGGTTTCCAG GAGTGGCCAGAATAACTCTGTGTTTACTCTGTATGAACTGACGAGTGGGGAAGACACAGAGGATGAAGGA ATGCCCAGGAATTGTGAGGCACAGCTGCAGCAGTGCACACAGCATACAAATAGATTATCCTGGCTTTGGA AGTCTCAGCTTCTGGCTCAGCCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228270 representing NM_001284412 Red=Cloning site Green=Tags(s) MAMSFEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVKLQRKLP VESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTSGEDTEDEG MPRNCEAQLQQCTQHTNRLSWLWKSQLLAQPQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284412
ORF Size	516 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284412.2
RefSeq Size	1259 bp
RefSeq ORF	519 bp
Locus ID	28084
MW	20.9 kDa
Gene Summary	Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL (By similarity).[UniProtKB/Swiss-Prot Function]

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