Arl4a (NM_007487) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI467555; Arl4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228463 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAATGGACTGTCAGACCAGACTTCCATCCTGTCCAGCCTGCCGTCCTTTCAGTCCTTTCACATTG TTATTCTGGGTTTGGACTGTGCTGGAAAGACAACAGTTTTATACAGGCTGCAGTTCAACGAATTTGTAAA TACCGTACCTACCAAAGGATTTAACACTGAGAAAATTAAGGTAACCTTGGGCAATTCCAAAACAGTCACT TTTCACTTCTGGGATGTAGGTGGTCAAGAGAAGTTAAGACCACTGTGGAAGTCATACACCCGATGCACAG ATGGTATTGTGTTTGTGGTGGACTCTGTCGATGTTGAGAGAATGGAAGAAGCCAAAACTGAACTTCATAA AATAACTAGGATATCCGAAAATCAGGGAGTCCCTGTGCTTATAGTTGCTAATAAACAAGACCTGAGGAAC TCGCTCTCTCTCTCAGAGATTGAGAAGTTGTTAGCAATGGGGGAACTGAGCTCATCGACTCCTTGGCATT TGCAGCCCACCTGTGCAATCATAGGAGATGGGCTAAAGGAAGGACTCGAGAAACTACATGATATGATAAT TAAAAGGAGAAAAATGTTGCGGCAACAGAAAAAGAAGAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228463 protein sequence Red=Cloning site Green=Tags(s) MGNGLSDQTSILSSLPSFQSFHIVILGLDCAGKTTVLYRLQFNEFVNTVPTKGFNTEKIKVTLGNSKTVT FHFWDVGGQEKLRPLWKSYTRCTDGIVFVVDSVDVERMEEAKTELHKITRISENQGVPVLIVANKQDLRN SLSLSEIEKLLAMGELSSSTPWHLQPTCAIIGDGLKEGLEKLHDMIIKRRKMLRQQKKKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_007487
ORF Size	603 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_007487.3, NP_031513.1
RefSeq Size	3657 bp
RefSeq ORF	603 bp
Locus ID	11861
UniProt ID	P61213
MW	22.6 kDa
Gene Summary	Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Recruits CYTH1, CYTH2, CYTH3 and CYTH4 to the plasma membrane in GDP-bound form (By similarity).[UniProtKB/Swiss-Prot Function]

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