Arhgdib (NM_001301305) Mouse Tagged ORF Clone
CAT#: MR228469
- TrueORF®
Arhgdib (myc-DDK-tagged) - Mouse Rho, GDP dissociation inhibitor (GDI) beta (Arhgdib), transcript variant 4
"NM_001301305" in other vectors (1)
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CNY 2,400.00
CNY 3,810.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D4; Gdid; Gdid4; Ly-G; Ly-GDI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228469 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGAGAAGGATGCACAGCCACAGCTGGAAGAGGCGGACGACGACCTGGACAGCAAGCTCAATTATA AGCCACCCCCTCAGAAGTCCTTGAAGGAGCTGCAGGAGATGGACAAGGATGACGAGAGTCTAACCAAGTA CAAGAAAACACTGCTGGGAGATGTCCCTGTGGTAGCAGACCCAACAGTTCCCAACGTGACTGTTACCCGG CTTAGCCTTGTATGTGACAGTGCACCAGGACCCATCACCATGGACCTTACTGGCGATCTCGAGGCCCTCA AAAAGGATACATTTGTGCTAAAGGAAGGCATTGAATACAGGGTGAAAATTAACTTCAAAGTGAATAAGGA TATTGTGTCTGGCCTGAAGTATGTTCAACACACATACCGGACTGGCATGAGAGTGGATAAAGCCACATTC ATGGTTGGCAGCTATGGGCCCCGACCAGAGGAGTACGAATTCCTCACTCCAGTAGAGGAAGCTCCCAAGG GCATGCTGGCCCGAGGCACTTACCACAACAAGTCCTTCTTCACGGATGACGACAAACAGGACCACCTCAC CTGGGAATGGAACCTGGCCATTAAGAAGGATTGGACAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228469 protein sequence
Red=Cloning site Green=Tags(s) MTEKDAQPQLEEADDDLDSKLNYKPPPQKSLKELQEMDKDDESLTKYKKTLLGDVPVVADPTVPNVTVTR LSLVCDSAPGPITMDLTGDLEALKKDTFVLKEGIEYRVKINFKVNKDIVSGLKYVQHTYRTGMRVDKATF MVGSYGPRPEEYEFLTPVEEAPKGMLARGTYHNKSFFTDDDKQDHLTWEWNLAIKKDWTE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001301305 |
ORF Size | 600 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001301305.1, NP_001288234.1 |
RefSeq Size | 1162 bp |
RefSeq ORF | 603 bp |
Locus ID | 11857 |
UniProt ID | Q61599 |
MW | 22.9 kDa |
Gene Summary | The protein encoded by this gene is a member of the Rho guanine nucleotide dissociation inhibitor (GDI) family. This gene is expressed at high levels in hematopoietic cells. This protein is cytosolic, and dissociation of Rho from this protein is required for membrane association and activation of Rho by Guanine Nucleotide Exchange Factors (GEFs). C-terminal truncations of this gene product have been reported to promote metastasis. Multiple transcript variants and protein isoforms exist. [provided by RefSeq, Aug 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226258 | Arhgdib (untagged) - Mouse Rho, GDP dissociation inhibitor (GDI) beta (Arhgdib), transcript variant 4 |
CNY 2,000.00 |
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