Ube2t (NM_001278115) Mouse Tagged ORF Clone
CAT#: MR228493
- TrueORF®
Ube2t (myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2T (putative) (Ube2t), transcript variant 2
"NM_001278115" in other vectors (1)
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CNY 2,400.00
CNY 3,810.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2700084L22Rik; C80607 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228493 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGAGAGCCTCACGCCTGAAGAAGGAACTGCACATGCTAGCCATCGAACCTCCCCCGGGCATCACGT GCTGGCAGGAAAAGGATCAAGTGGCTGATTTGCGAGCTCAAATACTAGGTGGAGCTAATACACCTTATGA GAAAGGTGTTTTCACACTGGAAGTTATCATTCCTGAGAGGTACCCATTTGAACCTCCACAGGTCCGATTT CTAACTCCAATCTATCATCCAAACATTGATTCTAGTGGAAGGATTTGTCTAGATATTCTCAAATTGCCAC CAAAGGGTGCATGGAGACCATCCCTCAACATTGCCACTGTATTGACCTCTATTCAGCTGCTCATGGCAGA ACCCAATCCTGATGACCCACTAATGGCTGACATTTCTTCAGAATTTAAATATAACAAGATAGCTTTTCTC AAGAAAGCCAAGCAGTGGACAGAGGCTCATGCAAGACAGAAACAAAAGGCTGATGAAGAGGAGCTAGGCA CCTCATCAGAAGTTGGTGACTCTGAGGAAAGCCATTCCACACAGAAAAGGAAGGCCAGGCCATTAGGAGG AATGGAAAAAAAGTTTTCCCCTGACGTTCAGAGAGTCTATCCTGGTCCCTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228493 protein sequence
Red=Cloning site Green=Tags(s) MQRASRLKKELHMLAIEPPPGITCWQEKDQVADLRAQILGGANTPYEKGVFTLEVIIPERYPFEPPQVRF LTPIYHPNIDSSGRICLDILKLPPKGAWRPSLNIATVLTSIQLLMAEPNPDDPLMADISSEFKYNKIAFL KKAKQWTEAHARQKQKADEEELGTSSEVGDSEESHSTQKRKARPLGGMEKKFSPDVQRVYPGPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001278115 |
ORF Size | 615 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001278115.1, NP_001265044.1 |
RefSeq Size | 957 bp |
RefSeq ORF | 615 bp |
Locus ID | 67196 |
UniProt ID | Q9CQ37 |
MW | 23 kDa |
Gene Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair: acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226282 | Ube2t (untagged) - Mouse ubiquitin-conjugating enzyme E2T (putative) (Ube2t), transcript variant 2 |
CNY 2,000.00 |