Ndufs8 (NM_001271443) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	BC021616; CI-23kD; TYKY
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR228537 representing NM_001271443 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATCGCCTGAGCTCATCAATGCTGCCACGGGCCTTGGCCCAGGCCATGCGCACAGGACATCTTAATG GACAAAGCCTTCATAGCAGCGCAGTGGCGGCAACGTACAAGTATGTGAATAAGAAGGAACAGGAGTCTGA GGTGGACATGAAGTCCGCAACTGACAATGCAGCTCGGATTCTGATGTGGACAGAACTCATCCGAGGACTG GGCATGACCCTAAGTTACCTCTTTCGAGAGCCTGCCACCATCAACTACCCCTTTGAGAAGGGCCCACTGA GTCCGCGCTTCCGTGGGGAGCATGCACTGCGCCGCTACCCATCTGGAGAGGAGCGTTGCATTGCCTGCAA ACTCTGTGAGGCCATCTGTCCTGCACAGGCCATCACCATTGAGGCTGAGCCAAGAGCCGATGGCAGCCGC CGAACGACACGCTATGACATCGACATGACCAAGTGTATCTACTGTGGTTTCTGCCAGGAAGCCTGCCCTG TTGATGCCATTGTGGAGGGCCCCAACTTCGAGTTCTCCACCGAGACACACGAGGAGTTGCTGTACAACAA GGAGAAGCTACTCAACAATGGAGACAAGTGGGAGGCCGAGATCGCTGCCAACATCCAGGCTGACTACCTG TACCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR228537 representing NM_001271443 Red=Cloning site Green=Tags(s) MYRLSSSMLPRALAQAMRTGHLNGQSLHSSAVAATYKYVNKKEQESEVDMKSATDNAARILMWTELIRGL GMTLSYLFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSR RTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYL YR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271443
ORF Size	636 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271443.1, NP_001258372.1
RefSeq Size	814 bp
RefSeq ORF	639 bp
Locus ID	225887
UniProt ID	Q8K3J1
MW	24 kDa
Gene Summary	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).[UniProtKB/Swiss-Prot Function]

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