Fcer2a (NM_001253743) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CD23; Fce2; Fcer2; Ly-42
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229125 representing NM_001253743 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAAATGAATACTCAGCAATGTGGGCTGGCCTGCTGGCCCTGCTTCTTCTGTGGCACTGGGAAA CGGAGAAGAATCTAAAACAGCTGGGAGACACTGCAATTCAGAATGTCTCTCATGTTACCAAGGACTTACA AAAATTCCAGAGTAATCAATTGGCCCAGAAGTCCCAGGTTGTTCAGATGTCACAAAACTTGCAAGAACTC CAAGCTGAACAGAAGCAAATGAAAGCTCAGGACTCTCGGCTCTCCCAGAACCTGACCGGACTCCAGGAGG ATCTAAGGAACGCCCAATCCCAGAACTCAAAACTCTCCCAGAACCTGAACAGACTCCAAGACGATCTAGT CAACATCAAATCCCTGGGCTTGAATGAGAAGCGCACAGCCTCCGATTCTCTAGAGAAACTCCAGGAAGAG GTGGCAAAGCTGTGGATAGAGATACTGATTTCAAAGGGAACTGCATGCAACATATGTCCCAAGAACTGGC TCCATTTCCAACAGAAGTGCTACTATTTTGGCAAGGGCTCCAAGCAGTGGATCCAGGCCAGGTTCGCCTG CAGTGACCTGCAAGGGCGACTAGTCAGCATCCACAGCCAAAAGGAACAGGACTTCCTGATGCAACACATC AACAAGAAGGATTCCTGGATTGGCCTCCAGGATCTCAATATGGAGGGAGAGTTTGTATGGTCGGACGGGA GCCCTGTGGGTTATAGCAACTGGAATCCAGGGGAGCCCAATAACGGGGGCCAGGGTGAGGACTGTGTGAT GATGCGGGGATCCGGCCAGTGGAACGACGCCTTCTGCCGCAGCTACTTGGATGCATGGGTGTGTGAGCAG CTGGCAACATGTGAGATATCTGCCCCCTTAGCCTCTGTGACTCCAACAAGGCCCACCCCAAAAAGTGAAC CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229125 representing NM_001253743 Red=Cloning site Green=Tags(s) MEENEYSAMWAGLLALLLLWHWETEKNLKQLGDTAIQNVSHVTKDLQKFQSNQLAQKSQVVQMSQNLQEL QAEQKQMKAQDSRLSQNLTGLQEDLRNAQSQNSKLSQNLNRLQDDLVNIKSLGLNEKRTASDSLEKLQEE VAKLWIEILISKGTACNICPKNWLHFQQKCYYFGKGSKQWIQARFACSDLQGRLVSIHSQKEQDFLMQHI NKKDSWIGLQDLNMEGEFVWSDGSPVGYSNWNPGEPNNGGQGEDCVMMRGSGQWNDAFCRSYLDAWVCEQ LATCEISAPLASVTPTRPTPKSEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253743
ORF Size	912 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001253743.1, NP_001240672.1
RefSeq Size	2160 bp
RefSeq ORF	915 bp
Locus ID	14128
UniProt ID	P20693
MW	35 kDa
Gene Summary	Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen).[UniProtKB/Swiss-Prot Function]

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