Ppa2 (NM_001293641) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110013G13Rik; Sid6306
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229274 representing NM_001293641 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGCGCTGCTGCCGCTGCTGAGCGTCGGCCGAGGGTGGCGCGTGGGGGCCGCCGCCCGCCCTCCGC GCCGCGTCATGTCCCTGTACCGCACGGAGGAGCTGGGCCATCCCCGCTCGCAGGACTACCGCCTGTTCTT CAAGCATGTAGCTGGTCATTACATCTCCCCTTTCCACGACATTCCTCTGAAGGCGGACTGTAAAGAGGAA CATGACATTCCCAGGAAAAAGGCACGAAATGATGAATATGAGAATTTGTTTAATATGGTTGTGGAAATAC CTCGGTGGACAAATGCCAAGATGGAGATTGCCACAGAGGAGCCACTCAATCCCATTAAGCAAGATATAAA GAACGGAAAGCTACGCTACACACCGAACATCTTCCCTCACAAGGGCTATATATGGAATTACGGGGCCCTC CCTCAGACCTGGGAAGATCCTCACCTAAGAGATAAGAGCACCGACTGCTGTGGGGATAATGATCCCATTG ACGTCTGTGAAATAGGCTCAAAGGTTCTTTCCCGTGGAGACGTGGTCCATGTGAAGATCCTTGGGACTTT GGCACTTATTGATCAGAGTGAAACAGATTGGAAAATAATTGCTATCAATGTGAATGATCCTGAGGCTGAA AAATTTCATGATATTGATGATGTTAAGAAGTTCAAACCAGGTTACCTGGAAGCCACTCTCAATTGGTTTA GACTCTATAAGGTACCAGATGGAAAACCAGAAAACAAGTTTGCTTTTAATGGAGAATTCAAAAACAAGGC TTTCGCCCTCGACGTTATTAACTCTGCACATGAACGTTGGAAAGAGATGGTTATGAAGAAGTGTGACAAG GGAGCCATCAGTTGTGTAAATGTGCACATCTGCGATAGCCCTTTCCACTGCACTATGGAGGAAGCAAGAT CATTGGTTGAATCGGTTCCAACACCTTCGATGAATAAGGAAAGCAATGTAGAGGAAGTGTGGCACTTCCT CCGCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229274 representing NM_001293641 Red=Cloning site Green=Tags(s) MRALLPLLSVGRGWRVGAAARPPRRVMSLYRTEELGHPRSQDYRLFFKHVAGHYISPFHDIPLKADCKEE HDIPRKKARNDEYENLFNMVVEIPRWTNAKMEIATEEPLNPIKQDIKNGKLRYTPNIFPHKGYIWNYGAL PQTWEDPHLRDKSTDCCGDNDPIDVCEIGSKVLSRGDVVHVKILGTLALIDQSETDWKIIAINVNDPEAE KFHDIDDVKKFKPGYLEATLNWFRLYKVPDGKPENKFAFNGEFKNKAFALDVINSAHERWKEMVMKKCDK GAISCVNVHICDSPFHCTMEEARSLVESVPTPSMNKESNVEEVWHFLRN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293641
ORF Size	987 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293641.1, NP_001280570.1
RefSeq Size	1380 bp
RefSeq ORF	990 bp
Locus ID	74776
UniProt ID	Q91VM9
MW	38.4 kDa
Gene Summary	Hydrolyzes inorganic pyrophosphate. This activity is essential for correct regulation of mitochondrial membrane potential, and mitochondrial organization and function.[UniProtKB/Swiss-Prot Function]

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