Aga (NM_001205054) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW060726
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229318 representing NM_001205054 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGGAAGTCGAATCTGTCTCTGCTTCTCCTACTGCTGGTCCTGGGCATGCCCCTGGTGCGGGGCT CCAGCCCTCTGCCCCTGGTCGTCAACACTTGGCCTTTTAAGAATGCCACTGAAGCAGCGTGGTGGACATT GCTATCTGGAGGTTCTGCCCTGGATGCAGTGGAGAACGGCTGTGCTGTGTGTGAGAAGGAGCAGTGTGAT GGGACTGTAGGCTTTGGAGGAAGTCCTGATGAAGGTGGCGAAACCACCCTGGATGCCATGATAATGGATG GCACTGCCATGGATGTGGGAGCAGTGGGAGGCCTTAGAAGAATTAAAAACGCGATTGGCGTGGCGCGGAG AGTCCTGGAGCATACCACACACACGCTTTTAGTGGGGGACTCAGCCACCAAGTTTGCTGAAAGTATGGGG TTTACTAATGAGGACTTGTCTACCAAAACCTCAAGAGATCTTCATTCAGATTGGCTTTCTCGAAATTGCC AGCCAAATTATTGGAGAAATGTTATTCCAGATCCCTCAAAATACTGTGGACCCTACAAACCATCTGGTTT CTTAAAGCAGAGTATTTCTCCCCACAAAGAAGAAGTGGATATCCACAGCCATGATACTATTGGCATGGTT GTAATCCATAAGACGGGACATACTGCTGCTGGCACATCCACAAATGGGGATTCACCAATCCCTGGAGCCG GAGCCTATGCTGATGACACGGCTGGAGCAGCTGCAGCCACTGGCGATGGTGACACACTCCTGCGCTTTCT GCCGAGCTACCAAGCTGTAGAATATATGAGAGGAGGAGATGACCCAGCCATAGCTTGCCAAAAAGTGATT TTAAGAATTCAGAAATACTATCCAAACTTCTTTGGAGCGGTCATATGTGCCAGTGTGAACGGAAGTTATG GTGCTGCTTGCAACAAACTTCCAACATTTACACAATTTAGTTTCATGGTTTCTAATTCTTTACACAATGA GCCAACCGAAAAAAAAGTAGACTGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229318 representing NM_001205054 Red=Cloning site Green=Tags(s) MERKSNLSLLLLLLVLGMPLVRGSSPLPLVVNTWPFKNATEAAWWTLLSGGSALDAVENGCAVCEKEQCD GTVGFGGSPDEGGETTLDAMIMDGTAMDVGAVGGLRRIKNAIGVARRVLEHTTHTLLVGDSATKFAESMG FTNEDLSTKTSRDLHSDWLSRNCQPNYWRNVIPDPSKYCGPYKPSGFLKQSISPHKEEVDIHSHDTIGMV VIHKTGHTAAGTSTNGDSPIPGAGAYADDTAGAAAATGDGDTLLRFLPSYQAVEYMRGGDDPAIACQKVI LRIQKYYPNFFGAVICASVNGSYGAACNKLPTFTQFSFMVSNSLHNEPTEKKVDCI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205054
ORF Size	1008 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205054.1, NP_001191983.1
RefSeq Size	1277 bp
RefSeq ORF	1011 bp
Locus ID	11593
MW	36.4 kDa
Gene Summary	This gene encodes an amidase enzyme that participates in the breakdown of glycoproteins in the cell. The encoded protein undergoes proteolytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit accumulation of aspartylglucosamine along with lysosomal vacuolization, axonal swelling in the gracile nucleus and impaired neuromotor coordination. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

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