Mest (NM_001252292) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA408879; AI256745; Peg1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229365 representing NM_001252292 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGTGTTAGAACCCAGGAGCAGCTCTCTGCTTAAGGCCATGCAGATGAGAGAGTGGTGGGTCCAAG TAGGGCTCCTGGCTGTGCCCTTGCTGGCTGCGTACCTGCACATCCCGCCCCCTCAGCTCTCCCCTGCTCT GCACTCATGGAAGACTTCTGGCAAGTTTTTCACCTACAAAGGCCTACGCATCTTCTACCAAGATTCTGTC GGTGTGGTCGGAAGCCCTGAGATAGTTGTGCTTTTACATGGCTTTCCAACATCCAGCTATGACTGGTACA AGATCTGGGAAGGGCTGACCCTGAGGTTCCATCGAGTGATCGCCCTTGATTTCTTAGGCTTTGGCTTCAG TGACAAGCCGAGACCACATCAGTACTCCATATTTGAGCAGGCCAGCATCGTAGAGTCCCTTCTGCGGCAC CTGGGGCTCCAGAACCGCAGAATCAACCTGCTGTCTCACGATTATGGAGATATCGTTGCTCAGGAACTGC TGTACAGGTACAAGCAGAATCGATCTGGCCGGCTCACCATAAAGAGTCTCTGTCTGTCGAATGGAGGTAT CTTTCCTGAGACGCATCGTCCTCTCCTTCTCCAAAAGCTCCTCAAAGACGGAGGTGTGCTTTCCCCCATC CTCACCAGGCTCATGAATTTCTTCGTGTTCTCTCGAGGTCTCACTCCAGTCTTTGGACCGTATACTCGAC CCACTGAGAGTGAGCTGTGGGACATGTGGGCTGTGATTCGCAACAATGACGGCAACCTGGTCATCGACAG TCTTTTACAGTACATTAATCAAAGGAAGAAATTCAGAAGACGCTGGGTGGGAGCGCTTGCTTCTGTGTCC ATCCCCATTCATTTTATCTATGGGCCATTGGATCCTATAAATCCGTATCCAGAGTTTTTGGAGCTGTACA GGAAAACGCTGCCGCGGTCCACAGTGTCGATTCTGGATGACCACATTAGCCACTACCCACAGCTAGAGGA TCCCATGGGCTTCTTGAATGCATATATGGGCTTCATCAACTCCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229365 representing NM_001252292 Red=Cloning site Green=Tags(s) MYVLEPRSSSLLKAMQMREWWVQVGLLAVPLLAAYLHIPPPQLSPALHSWKTSGKFFTYKGLRIFYQDSV GVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHQYSIFEQASIVESLLRH LGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPLLLQKLLKDGGVLSPI LTRLMNFFVFSRGLTPVFGPYTRPTESELWDMWAVIRNNDGNLVIDSLLQYINQRKKFRRRWVGALASVS IPIHFIYGPLDPINPYPEFLELYRKTLPRSTVSILDDHISHYPQLEDPMGFLNAYMGFINSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252292
ORF Size	1026 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252292.1, NP_001239221.1
RefSeq Size	2680 bp
RefSeq ORF	1029 bp
Locus ID	17294
UniProt ID	Q07646
MW	40 kDa

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