Txndc5 (NM_001289599) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AL022641; ERp46; PC-TRP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229376 representing NM_001289599 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGGACACTGCCAGCGGCTGCAGCCAACTTGGAATGACCTGGGAGACAAGTACAACAGCATGGAGG ATGCCAAGGTCTACGTGGCCAAAGTGGACTGCACGGCTGATTCCGACGTGTGCTCTGCCCAGGGAGTGCG AGGATACCCCACCCTGAAGTTTTTTAAGCCTGGACAAGAAGCAGTGAAGTACCAGGGTCCTAGAGACTTT GAAACACTGGAAAACTGGATGCTGCAGACACTGAACGAGGAGCCAGCCACACCGGAGCCGGAAGCGGAAC CACCCAGAGCCCCTGAGCTCAAACAGGGGTTGTATGAGCTCTCGGCCAACAACTTTGAGCTGCATGTTTC TCAAGGCAACCACTTTATCAAGTTCTTCGCTCCGTGGTGCGGTCACTGCAAAGCTCTGGCTCCAACCTGG GAGCAGCTGGCTCTGGGCCTTGAACATTCTGAAACCGTCAAGATTGGCAAGGTTGACTGCACGCAGCACT ACGCTGTCTGCTCAGAGCATCAGGTCAGAGGCTATCCAACTCTGCTCTGGTTTCGAGATGGCAAGAAGGT GGATCAGTACAAGGGAAAGCGGGACTTGGAGTCACTGAGAGACTATGTGCAGTCCCAGCTGCAGGGTTCA GAGGCAGCTCCGGAGACTGTTGAGCCGTCAGAGGCCCCAGTGATGGCTGCTGAGCCCACGGGTGACAAGG GCACTGTGCTGGCACTCACCGAGAAGAGCTTCGAGGACACTATTGCACAGGGGATAACCTTCGTCAAGTT CTATGCTCCGTGGTGTGGCCACTGTAAGAATCTGGCTCCTACCTGGGAGGAGCTCTCTAAAAAGGAATTC CCAGGCTTGTCAGATGTCACCATCGCAGAAGTGGACTGCACCGCTGAGCGCAATGTCTGCAGCAAGTACT CGGTACGAGGTTATCCCACGTTGCTGCTTTTCCGAGGAGGTGAAAAAGTGGGAGAACACAACGGAGGTAG AGACCTCGACTCCTTACACAGCTTTGTTCTGCGCCAGGCAAAGGATGAACTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229376 representing NM_001289599 Red=Cloning site Green=Tags(s) MCGHCQRLQPTWNDLGDKYNSMEDAKVYVAKVDCTADSDVCSAQGVRGYPTLKFFKPGQEAVKYQGPRDF ETLENWMLQTLNEEPATPEPEAEPPRAPELKQGLYELSANNFELHVSQGNHFIKFFAPWCGHCKALAPTW EQLALGLEHSETVKIGKVDCTQHYAVCSEHQVRGYPTLLWFRDGKKVDQYKGKRDLESLRDYVQSQLQGS EAAPETVEPSEAPVMAAEPTGDKGTVLALTEKSFEDTIAQGITFVKFYAPWCGHCKNLAPTWEELSKKEF PGLSDVTIAEVDCTAERNVCSKYSVRGYPTLLLFRGGEKVGEHNGGRDLDSLHSFVLRQAKDEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289599
ORF Size	1032 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289599.1, NP_001276528.1
RefSeq Size	2614 bp
RefSeq ORF	1035 bp
Locus ID	105245
UniProt ID	Q91W90
MW	39 kDa
Gene Summary	Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast.[UniProtKB/Swiss-Prot Function]

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