Mdm4 (NM_001302804) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229495 representing NM_001302804 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGTGCAACAGAATACTCCAATCCCAGAAAAAGAACTG AAGAAGAGGATACTCACACACTGCCTACCTCACGACATAAATGCAGAGACTCCAGAGCAGATGAAGACTT GATAGAACATTTATCTCAAGATGAGACATCTAGGCTTGACCTTGATTTTGAGGAGTGGGACGTTGCTGGC CTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAACTGTATTCCTAAAAGTAATGGCTCAACTGATTTAC AGACAAATCAGGATATAGGTACTGCCATTGTTTCAGACACTACGGATGATTTGTGGTTTTTAAATGAGAC CGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGAAGCTGCTAATTCTGAGCAAACAAGTGAAGTAGGG AAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAGGATGATGATCTTGAGGACTCCAGGTCCTTGAGCG ATGATACTGACGTGGAACTTACCTCTGAGGATGAGTGGCAGTGTACGGAATGCAAGAAGTTTAATTCTCC AAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAGAAAGGATTGGTATTCGGATTGTTCTAAATTAACT CATTCCCTATCTACATCTAATATTACTGCCATACCTGAAAAGAAGGACAATGAAGGAATTGATGTTCCCG ATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGCCTAAAGATGGATATTTAAAGGAGGAAAAGCCCAG GTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTTGGCTCATAGTTCTGAAAGCCAGGAGATCATCTCA AGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAGAAAGCTGAAACAGAAAGTATGGAAGATTTCCAGA ATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGCCTCGGGATGGGAACATTATTCATGGGAAGACGAG CCATCTGACGACATGTTTCCACTGTGCCAGGAGACTGAAGAAGTCTGGGGCTTCGTGTCCTGCTTGTAAG AAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229495 representing NM_001302804 Red=Cloning site Green=Tags(s) MDFPSQDRLKHGATEYSNPRKRTEEEDTHTLPTSRHKCRDSRADEDLIEHLSQDETSRLDLDFEEWDVAG LPWWFLGNLRNNCIPKSNGSTDLQTNQDIGTAIVSDTTDDLWFLNETVSEQLGVGIKVEAANSEQTSEVG KTSNKKTVEVGKDDDLEDSRSLSDDTDVELTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLT HSLSTSNITAIPEKKDNEGIDVPDCRRTISAPVVRPKDGYLKEEKPRFDPCNSVGFLDLAHSSESQEIIS SAREQTDIFSEQKAETESMEDFQNVLKPCSLCEKRPRDGNIIHGKTSHLTTCFHCARRLKKSGASCPACK KEIQLVIKVFIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302804
ORF Size	1086 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302804.1, NP_001289733.1
RefSeq Size	7194 bp
RefSeq ORF	1089 bp
Locus ID	17248
UniProt ID	O35618
MW	41.5 kDa
Gene Summary	This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014]

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