Tm6sf2 (NM_001293795) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229584 representing NM_001293795 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACATCCCGCCCCTAGCCGGCAGGACTGTGGCAATGTCATTCTGCGCCCTGCCAGTGTCCTACCTAC TCAATCAAGTTTCGGCGTTCTCACAGCCCCTGTTTGTGGTGCTGACGAGTGCTCTGATACTGGGTCTGCT CTTCCTGGCAGTCTATAGCCTATCTCATGGGGAGATCACCTACGACCCTCTCTATGCTGTCTTTGTGATC TTCTCCTTCACCTCGGTGGTGGACCTTGTCATTGCACTCCAAGAAGATGGCTACATGATGGGCTTCATGG ATTTCTACACCAAGGAGGGTGAGCCCTACCTACGCACAGCACATGGCATCTTCATCTGCTACTGGGATGG TACTGTGCACTACCTGCTCTATCTGACAATGGCTGGTGCCATCCGCAAAAGGAAAAGGTACCGGAACCTT GGGCTCTACTGGCTGGGATCCTTCGCCATGAGCATCCTGGTGTTTCTCCCGGGAAACATCCTTGGTAAAT ACAGTTCAGAGATGAGGCCTACCTTCTTCCTCGCCATCCTGTACATGCTGGTCCCATGCTGGGCCGGTGT GAGGATCTTCAACCAGTCCCGGGCACCAACCTCCTATACCCCTGACATGGTACAGGAAGAACAGAAGAAA AGTCTCTTCCAGCGTCCGGCTGACCTGGCACTCATAGTATACCTCATCTTTGCAGCGCTCTTCACTGTCT TCCGGGGTCTGGTGGTACTTGACTGCCCCACAGATGCCTGCTTCATCTATATTTACCAATATGAGCCATA CCTTCGGGACCCTGTGGCCTACCCAAAGTTGCAGATGCTGATGTATTTGTTCTATGCTCTACCATTCTAC TGCCTGGCTGCCTACGCCCTTGCCTTCCCTGGCTGCTCCTGGCTGCCGGACTGGGCCTTGGTATTTGCTG GAGCCATTGGCCAGGCGCAGTTCTCACACATGGGTGCCTCCATGCACATGCGCACACCCTTCACCTACCG CGTGCCAGAGGACACCTGGGCTACCTTCTTTCTGAGCAACCTGCTATTGGCACTGGGTCCCCACCTGCTG GCTTTCCGCTGCCTGTGGCGGCCCGCCTTCTTCTTGCATGCAGCGCTGCCATCTTCGCCACAGGACCAAG ACAAGAAGCAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229584 representing NM_001293795 Red=Cloning site Green=Tags(s) MDIPPLAGRTVAMSFCALPVSYLLNQVSAFSQPLFVVLTSALILGLLFLAVYSLSHGEITYDPLYAVFVI FSFTSVVDLVIALQEDGYMMGFMDFYTKEGEPYLRTAHGIFICYWDGTVHYLLYLTMAGAIRKRKRYRNL GLYWLGSFAMSILVFLPGNILGKYSSEMRPTFFLAILYMLVPCWAGVRIFNQSRAPTSYTPDMVQEEQKK SLFQRPADLALIVYLIFAALFTVFRGLVVLDCPTDACFIYIYQYEPYLRDPVAYPKLQMLMYLFYALPFY CLAAYALAFPGCSWLPDWALVFAGAIGQAQFSHMGASMHMRTPFTYRVPEDTWATFFLSNLLLALGPHLL AFRCLWRPAFFLHAALPSSPQDQDKKQQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293795
ORF Size	1134 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293795.1, NP_001280724.1
RefSeq Size	1446 bp
RefSeq ORF	1137 bp
Locus ID	107770
MW	43.5 kDa
Gene Summary	Regulator of liver fat metabolism influencing triglyceride secretion and hepatic lipid droplet content. May function as sterol isomerase.[UniProtKB/Swiss-Prot Function]

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