Nlrc3 (NM_175547) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CLR16.2; D230007K08Rik; mFLJ00348
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229695 representing NM_175547 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGAAGCCTGGCTGAGAATCAGATTGGTAACAAAGGAGCCAAAGCCCTGGCCAGATCCCTCCTGG TTAACAGAAGCCTCATCACACTGGACCTCCGGAGTAACAGCATTGGACCACCGGGGGCTAAGGCTTTGGC CGATGCTCTGAAGATAAACCGAACGCTAACTTCTCTAAGCCTCCAAAGCAACGTGATCAAGGATGACGGT GTCATGTGCGTGGCTGAGGCCCTGGTCTCCAACCAGACCATCTCCATGCTACAGCTACAGAAGAACTTAA TTGGGCTCATAGGAGCCCAGCAGATGGCAGATGCCCTGAAGCAGAACAGGAGCCTGAAAGCACTCATGTT TTCCAGTAATACCATTGGCGACAGAGGTGCCATAGCCCTGGCTGAGGCCCTGAAGGTGAACCAGATCCTG GAGAACTTAGACCTACAGAGCAATTCCATCAGTGACATGGGAGTGACGGTGCTGATGCGAGCCCTCTGCA GTAACCAGACACTCTCCAGTCTCAACCTGCGAGAAAACTCCATCAGCCCAGAGGGAGCCCAGGCCCTCAC TCAAGCTCTCTGCAGGAACAACACTCTGAAGCACTTGGACCTGACAGCTAATCTCCTCCATGACCGAGGT GCCCAGGCCATTGCAGTAGCTGTGGGAGAAAACCACTCCCTCACACACCTTCACCTGCAGTGGAACTTCA TCCAAGCTGGTGCGGCCAGGGCCCTGGGACAAGCACTCCAGCTGAACAGAACCCTGACAACCTTAGACTT ACAGGAGAATGCCATAGGGGATGAAGGAGCTTCCTCAGTGGCTGGCGCACTGAAGGTGAACACAACCCTC ATTGCTCTCTACCTACAGGTGGCCTCCATTGGTAGCCAAGGGGCCCAGGCACTTGGGGAGGCCCTCACTG TGAACAGAACCTTGGAGATTCTTGACTTACGAGGAAACGACGTTGGGGCAGCTGGAGCCAAGGCCTTGGC AAATGCTTTAAAGTTAAACTCCAGTCTCCGAAGACTCAATCTCCAGGAGAACTCACTGGGGATGGATGGG GCCATATTTGTTGCCTCTGCACTGTCTGAGAACCACGGTCTGCACCATATTAATCTCCAGGGGAATCCCA TTGGGGAGTCTGCTGCCAGGATGATCTCAGAGGCCATCAAGACAAACGCTCCCACATGCACTGTGGAAAT A ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229695 representing NM_175547 Red=Cloning site Green=Tags(s) MAGSLAENQIGNKGAKALARSLLVNRSLITLDLRSNSIGPPGAKALADALKINRTLTSLSLQSNVIKDDG VMCVAEALVSNQTISMLQLQKNLIGLIGAQQMADALKQNRSLKALMFSSNTIGDRGAIALAEALKVNQIL ENLDLQSNSISDMGVTVLMRALCSNQTLSSLNLRENSISPEGAQALTQALCRNNTLKHLDLTANLLHDRG AQAIAVAVGENHSLTHLHLQWNFIQAGAARALGQALQLNRTLTTLDLQENAIGDEGASSVAGALKVNTTL IALYLQVASIGSQGAQALGEALTVNRTLEILDLRGNDVGAAGAKALANALKLNSSLRRLNLQENSLGMDG AIFVASALSENHGLHHINLQGNPIGESAARMISEAIKTNAPTCTVEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_175547
ORF Size	1191 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_175547.4
RefSeq Size	2003 bp
RefSeq ORF	1194 bp
Locus ID	268857
UniProt ID	Q5DU56
MW	42.1 kDa
Gene Summary	Negative regulator of the innate immune response. Attenuates signaling pathways activated by Toll-like receptors (TLRs) and the DNA sensor STING/TMEM173 in response to pathogen-associated molecular patterns, such as intracellular poly(dA:dT), but not poly(I:C), or in response to DNA virus infection, including that of Herpes simplex virus 1 (HSV1) (PubMed:22863753, PubMed:24560620). May affect TLR4 signaling by acting at the level of TRAF6 ubiquitination, decreasing the activating 'Lys-63'-linked ubiquitination and leaving unchanged the degradative 'Lys-48'-linked ubiquitination (PubMed:22863753). Inhibits the PI3K-AKT-mTOR pathway possibly by directly interacting with the posphatidylinositol 3-kinase regulatory subunit p85 (PIK3R1/PIK3R2) and disrupting the association between PIK3R1/PIK3R2 and the catalytic subunit p110 (PIK3CA/PIK3CB/PIK3CD) and reducing PIK3R1/PIK3R2 activation. Via its regulation of the PI3K-AKT-mTOR pathway, controls cell proliferation, predominantly in intestinal epithelial cells (PubMed:27951586). May also affect NOD1- or NOD2-mediated NF-kappa-B activation (By similarity). Might also affect the inflammatory response by preventing NLRP3 inflammasome formation, CASP1 cleavage and IL1B maturation (By similarity).[UniProtKB/Swiss-Prot Function]

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