Sh2b2 (NM_001302939) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Aps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229819 representing NM_001302939 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGTGCCACCCCCAGCTCGGCCGCCGCCCCCGCCCCAGTCCCCGACTGGCGGCAATTCTGCGAGC TGCACGCGCAGGTGGCTGCTGTGGACTTCGCACACAAGTTCTGCCGCTTCCTTCGGGACAACCCGACCTA CGACACGCCTGACGCTGGGACCTCATTCTCCCGCCACTTTGCAGCCAACTTCTTGGCTGTCTTCAGTGAG GAGGTGCGCCGCGTGCTGGGGTCAGCAGCCGACACTATGGAACCCGAGCCTGCGGTGACCTCGGTGACAT CTGCGCTCAAGACTGCAACCTATGGCCACTCCCGCAGCTCCGAGGATGTGTCTGCCCACGCGGCTACCAA GGCGCGTGTCCGAAAGGGATTCTCGCTGCGTAACATGAGCCTGTGTGTGGTGGATGGAGTGCGCGACCTG TGGCACCGGCGCTCGTCCCCAGAGCCCGATGGTGGGGCCACCCCCAAGGCCGCCGAGCCTGCGTCGGAGC CTCGTGACAAATGGACGCGACGCCTGCGGCTAGCACGAACCCTGGCAGCCAAAGTGGAGTTAGTGGACAT CCAGCGTGAGGGCGCCCTGCGCTTCATGGTGGCAGACGACGCGGCCTCAGGGCCTGGAGGCACTGCGCAG TGGCAGAAGTGTCGCCTGCTCCTGCGGAGGGCTGTGGCTGGGGAACGTTTCCGCCTGGAGTTCTTCGTGC CACCCAAGGCTTCCAGACCCAAGGTCAGCATCCCCCTGTCGGCCATCATTGAGGTCCGAACCACTATGCC CCTGGAAATGCCTGAAAAGGACAACACATTTGTGCTCAAGGTGGAGAACGGAGCAGAATACATCCTGGAA ACGATAGACTCACTGCAGAAGCACTCGTGGGTGGCTGACATCCAGGGCTGTGTGGATCCTGGGGACAGTG AGGAAGACACAGGACTGTCTTGTGCACGGGGAGGCTGCCTGGCCAGCCGCGTGGCCTCCTGCAGCTGTGA GCTCCTGACAGATGCAGACATGCCCCGGCCCCCCGAGACGACGACAGCGGTGGGTGCTGTGGTCACAGCT CCACATGGCCGAGCTCGTGACACCGTGGGAGAGTCCCTGGCCCACGTTCCCCTGGAGACTTTCCTGCAAA CCCTGGAGTCTTCAGGTGGTGTCAGTGAGAACAATAACCCAGGTACCACTGGGGTCCCCACCCCCTTCTA CAGGGACCCATGTGGAGCCTCAGAAGTGGTGACATTTCAGCCAGTTGTGTCTACAGTGGTTTTGGCTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229819 representing NM_001302939 Red=Cloning site Green=Tags(s) MNGATPSSAAAPAPVPDWRQFCELHAQVAAVDFAHKFCRFLRDNPTYDTPDAGTSFSRHFAANFLAVFSE EVRRVLGSAADTMEPEPAVTSVTSALKTATYGHSRSSEDVSAHAATKARVRKGFSLRNMSLCVVDGVRDL WHRRSSPEPDGGATPKAAEPASEPRDKWTRRLRLARTLAAKVELVDIQREGALRFMVADDAASGPGGTAQ WQKCRLLLRRAVAGERFRLEFFVPPKASRPKVSIPLSAIIEVRTTMPLEMPEKDNTFVLKVENGAEYILE TIDSLQKHSWVADIQGCVDPGDSEEDTGLSCARGGCLASRVASCSCELLTDADMPRPPETTTAVGAVVTA PHGRARDTVGESLAHVPLETFLQTLESSGGVSENNNPGTTGVPTPFYRDPCGASEVVTFQPVVSTVVLAF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302939
ORF Size	1260 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302939.1, NP_001289868.1
RefSeq Size	1894 bp
RefSeq ORF	1263 bp
Locus ID	23921
UniProt ID	Q9JID9
MW	45.7 kDa
Gene Summary	Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways. May be involved in coupling from immunoreceptor to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. May induce cytoskeletal reorganization via interaction with VAV3 (By similarity).[UniProtKB/Swiss-Prot Function]

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