Cnot6l (NM_001285514) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4932442K20Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR229992 representing NM_001285514 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCTCTCAGGGAATTGCTTTTAAATGACAATTATTTACGGGTGTTGCCTTATGAACTTGGCCGGC TCTTCCAGCTACAAACTCTAGGTTTAACAGGCAATCCTTTATCACAGGATATTATGAGCTTATACCAGGA CCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAATCTTGCAGTTCATCCAGAGCAGCTTCCT CCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTGCCATCAGCATCATTCACGGTTATGTGTT ACAATGTGTTATGTGATAAATATGCTACCAGGCAGCTATATGGCTATTGTCCGTCCTGGGCATTAAACTG GGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAACTGGGACGCAGATATCATTAGTCTTCAGGAA GTGGAAACAGAGCAATACTTTACTCTCTTTCTGCCAGCATTGAAGGATCGTGGATATGATGGATTTTTTT CTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAAAGAAAGCATGTGGATGGTTGTGCAATATTCTT CAAAACAGAAAAATTTACATTGGTGCAGAAGCATACAGTGGAATTCAACCAGGTAGCAATGGCAAATTCA GATGGATCCGAAGCAATGCTAAACAGAGTAATGACGAAAGATAACATTGGCGTTGCTGTGGTGTTAGAGG TCCACAAGGAGCTTTTTGGAACAGGTATGAAGCCTATTCATGCTGCAGACAAACAGCTGCTTATAGTGGC AAATGCCCACATGCATTGGGACCCAGAGTATTCTGATGTGAAACTTATTCAGACCATGATGTTTGTCTCA GAGGTTAAAAACATTCTGGAGAAAGCCTCAAGTAGGCCTGGCAGCCCAACTGCAGATCCCAATTCCATCC CGCTGGTGCTATGTGCAGATCTTAACTCATTGCCAGATTCAGGTGTTGTGGAATATTTAAGCAACGGTGG AGTAGCTGACAACCATAAAGACTTCAAGGAACTAAGGTACAATGAGTGTCTTATGAACTTCAGCTGTAGT GGAAAGAATGGAAGCTCAGAAGGGAGAATCACACATGGCTTCCAACTTAAGAGCGCCTATGAAAATAACT TGATGCCTTATACCAATTACACCTTTGATTTCAAAGGTGTGATTGACTACATTTTCTATTCCAAGACTCA TATGAACGTGCTTGGTGTCCTGGGGCCTTTAGATCCTCAATGGCTGGTTGAGAACAACATCACTGGGTGT CCACACCCTCACATCCCTTCAGACCACTTCTCACTGTTAACACAACTTGAACTCCACCCTCCACTCCTGC CTCTTGTCAATGGTGTTCACTTGCCTAATCGGAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR229992 representing NM_001285514 Red=Cloning site Green=Tags(s) MVSLRELLLNDNYLRVLPYELGRLFQLQTLGLTGNPLSQDIMSLYQDPDGTRKLLNFMLDNLAVHPEQLP PRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYCPSWALNWEYRKKGIMEEIVNWDADIISLQE VETEQYFTLFLPALKDRGYDGFFSPKSRAKIMSEQERKHVDGCAIFFKTEKFTLVQKHTVEFNQVAMANS DGSEAMLNRVMTKDNIGVAVVLEVHKELFGTGMKPIHAADKQLLIVANAHMHWDPEYSDVKLIQTMMFVS EVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVVEYLSNGGVADNHKDFKELRYNECLMNFSCS GKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDYIFYSKTHMNVLGVLGPLDPQWLVENNITGC PHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285514
ORF Size	1365 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285514.1, NP_001272443.1
RefSeq Size	8570 bp
RefSeq ORF	1368 bp
Locus ID	231464
UniProt ID	Q8VEG6
MW	52.2 kDa
Gene Summary	Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).[UniProtKB/Swiss-Prot Function]

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