Matk (NM_001285854) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CHK; HYL; Ntk; p56ntk
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230050 representing NM_001285854 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAACGCGCTGGGCCCCTGGGACTCAATGCATGACCAAGTGTGAGAACTCTCGCCCCAAGCCCGGTG AGCTAGCCTTTCGAAAGGGTGACATGGTGACCATCTTGGAGGCCTGTGAGGACAAGAGCTGGTACCGAGC CAAGCACCATGGCAGTGGGCAGGAAGGGCTGCTGGCGGCCGCTGCTCTGCGACAGCGGGAGGCCCTCTCC ACAGACCCCAAGCTCAGCCTCATGCCATGGTTTCATGGCAAGATCTCCGGCCAGGAAGCCATACAGCAGC TGCAGCCACCCGAGGACGGGCTGTTCCTTGTGAGGGAATCAGCTCGTCACCCTGGAGACTATGTCTTGTG TGTCAGTTTCGGCCGTGACGTCATCCACTACCGTGTTTTGCATCGAGATGGGCACCTCACCATCGATGAG GCCGTGTGTTTCTGTAACCTGATGGACATGGTGGAGCACTACACCAAGGACAAGGGGGCCATCTGCACCA AGCTGGTGAAGCCAAGGAGGAAACAGGGCGCAAAGTCTGCAGAGGAGGAGCTCGCCAAGGCTGGCTGGCT ACTCGACCTGCAGCATCTGACTCTGGGAGCACAGATTGGAGAGGGGGAGTTTGGAGCCGTCCTACAGGGT GAGTACCTGGGACAGAAGGTGGCTGTGAAGAATATCAAGTGTGATGTGACAGCCCAGGCCTTCCTGGATG AGACGGCTGTGATGACGAAGCTGCAGCACAGGAACCTAGTGCGACTCCTGGGTGTGATCCTGCACCACGG CTTGTACATTGTCATGGAGCACGTGAGCAAGGGCAACCTGGTGAACTTCCTGCGCACGCGGGGCCGTGCT CTTGTGAGCACCTCTCAGCTTCTGCAGTTTGCTCTTCATGTTGCTGAAGGCATGGAATACCTGGAGAGCA AGAAGCTGGTGCACCGGGACCTGGCTGCTCGGAACATCCTGGTCTCTGAGGACTTGGTGGCCAAGGTCAG TGACTTTGGCTTAGCCAAGGCAGAGCGCAAGGGGCTGGACTCAAGCCGGCTGCCAGTCAAGTGGACGGCA CCTGAGGCTCTCAAAAACGGGCGGTTCTCCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGTTGTGGG AAGTCTTCTCTTATGGAAGAGCCCCATACCCCAAGATGTCGCTAAAGGAGGTTTCAGAGGCTGTGGAGAA GGGTTACCGCATGGAGCCCCCCGATGGCTGCCCAGGCTCTGTGCACACCCTCATGGGTAGCTGCTGGGAG GCAGAGCCTGCGCGCCGACCACCCTTCCGCAAAATAGTGGAGAAGCTGGGCCGTGAGCTCCGCAGTGTGG GTGTCTCGGCCCCCGCTGGGGGACAGGAGGCTGAGGGCTCAGCTCCCACACGGAGCCAGGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230050 representing NM_001285854 Red=Cloning site Green=Tags(s) MPTRWAPGTQCMTKCENSRPKPGELAFRKGDMVTILEACEDKSWYRAKHHGSGQEGLLAAAALRQREALS TDPKLSLMPWFHGKISGQEAIQQLQPPEDGLFLVRESARHPGDYVLCVSFGRDVIHYRVLHRDGHLTIDE AVCFCNLMDMVEHYTKDKGAICTKLVKPRRKQGAKSAEEELAKAGWLLDLQHLTLGAQIGEGEFGAVLQG EYLGQKVAVKNIKCDVTAQAFLDETAVMTKLQHRNLVRLLGVILHHGLYIVMEHVSKGNLVNFLRTRGRA LVSTSQLLQFALHVAEGMEYLESKKLVHRDLAARNILVSEDLVAKVSDFGLAKAERKGLDSSRLPVKWTA PEALKNGRFSSKSDVWSFGVLLWEVFSYGRAPYPKMSLKEVSEAVEKGYRMEPPDGCPGSVHTLMGSCWE AEPARRPPFRKIVEKLGRELRSVGVSAPAGGQEAEGSAPTRSQDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001285854
ORF Size	1395 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285854.1, NP_001272783.1
RefSeq Size	1844 bp
RefSeq ORF	1398 bp
Locus ID	17179
UniProt ID	P41242
MW	52 kDa
Gene Summary	Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.[UniProtKB/Swiss-Prot Function]

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