Pi4kb (NM_001293716) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ESTM41; PI4K-beta; PI4Kbeta; Pik4cb
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230149 representing NM_001293716 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAATCGGCAAACGGCTGGCCACGCTCCCCACTAAAGAGCAAAAAACACAAAGGCTGATCTCAGAGC TTTCCCTGCTCAACCATAAGCTCCCTGCCCGAGTCTGGCTGCCCACGGCTGGCTTTGACCACCACGTGGT CCGTGTGCCCCACACACAAGCTGTTGTTCTCAATTCCAAGGATAAGGCTCCCTACCTGATCTACGTGGAA GTTCTCGAATGTGAAAACTTTGACACAACTAGTGTTCCTGCCCGGATTCCTGAAAACCGAATTCGGAGTA CACGGTCTGTAGAGAACCTGCCTGAATGTGGTATCACTCATGAGCAGCGAGCTGGCAGCTTCAGCACTGT GCCCAATTATGACAATGATGATGAAGCCTGGTCAGTGGATGATATAGGCGAGCTGCAAGTGGAGCTCCCT GAAGTGCACACCAACAGCTGTGACAACATCTCCCAGTTCTCGGTGGACAGCATCACCAGCCAGGAGAGCA AGGAGCCTGTGTTCATTGCAGCAGGGGACATCCGACGGCGCCTTTCAGAACAGCTGGCTCATACTCCTAC AGCCTTCAAACGAGACCCTGAAGACCCTTCTGCAGTTGCCCTCAAAGAGCCCTGGCAGGAGAAAGTGCGG AGGATCAGAGAAGGTTCCCCGTATGGCCATCTTCCCAATTGGCGACTCCTTTCAGTCATTGTCAAGTGTG GAGATGACCTTCGCCAGGAGCTGCTGGCTTTCCAGGTGTTGAAACAACTGCAGTCCATTTGGGAACAGGA GCGAGTGCCTCTTTGGATCAAGCCATATAAGATTCTTGTGATTTCAGCTGACAGTGGCATGATTGAACCA GTAGTCAACGCTGTGTCCATCCACCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTAC AGGAACATGGCAGTTATACCACTGAGGCATTCCTCAGTGCCCAGCGCAATTTTGTGCAAAGCTGTGCTGG CTACTGCTTGGTCTGCTACCTATTGCAAGTCAAGGACAGGCACAACGGGAACATCCTTCTGGACGCAGAA GGTCACATCATCCACATCGACTTTGGCTTCATCCTTTCCAGCTCACCCCGAAACCTGGGCTTCGAGACAT CAGCCTTTAAGCTGACCACAGAATTTGTGGATGTAATGGGTGGCCTGAACGGTGATATGTTCAACTACTA CAAGATGCTCATGCTGCAAGGGCTGATTGCTGCTCGGAAGCACATGGACAAGGTGGTACAGATTGTGGAG ATCATGCAGCAAGGTTCTCAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGCAACCTCAAAGAGAGGT TCCACATGAGCATGACTGAGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGGACGGCAGCATGAGGTC CATCACCACCAAACTCTACGATGGCTTCCAGTACCTCACCAATGGCATCATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230149 representing NM_001293716 Red=Cloning site Green=Tags(s) MAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVE VLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELP EVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVR RIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEP VVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAE GHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLNGDMFNYYKMLMLQGLIAARKHMDKVVQIVE IMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001293716
ORF Size	1452 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001293716.1, NP_001280645.1
RefSeq Size	2849 bp
RefSeq ORF	1455 bp
Locus ID	107650
UniProt ID	Q8BKC8
MW	55.4 kDa
Gene Summary	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation (By similarity). Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function]

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