St7 (NM_001289626) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	9430001H04Rik; Fam4a2; HELG; RAY1; SEN4; TSG7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230490 representing NM_001289626 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGAGGCGGGCGCGGGCTTCCTGGAGCAGCTCAAGTCCTGCATCGTGTGGTCGTGGACCTATCTGT GGACCGTGTGGTTCTTCCTCGTGCTCTTCCTGGTCTACATCCTGAGGGTGCCCCTGAGGATCAACGACAA CCTGAGCACAGTGAGCATGTTTTTGAACACGTTAACCCCAAAGTTCTACGTGGCCCTGACAGGCACTTCC TCGCTAATATCGGGACTTATTTTGATATTTGAATGGTGGTATTTTCGCAAGTATGGAACATCTTTCATTG AACAAGTCTCCGTAAGCCACTTGCGCCCCCTTCTGGGAGGGGTTGACAACAACTCTTCCAATAATTCTAA TTCCAGTAATGGAGACTCAGATTCCAACAGACAAAGTGTCTCAGAATGCAAAGTATGGCGAAATCCACTG AATCTGTTTAGGGGTGCGGAATATAATCGGTACACTTGGGTCACAGGACGAGAGCCTCTGACCTACTATG ACATGAACCTCTCTGCCCAGGACCACCAGACATTCTTTACTTGTGATTCAGATCATCTTCGCCCTGCAGA TGCAATAATGCAAAAAGCTTGGAGAGAAAGGAACCCTCAAGCCAGGATTTCTGCAGCTCATGAAGCCTTG GAGATAAACGAGTGTGCAACTGCGTACATTCTCTTGGCGGAAGAAGAAGCGACAACTATTGCTGAAGCAG AAAAACTCTTTAAGCAGGCCCTGAAGGCTGGAGATGGTTGTTACCGGCGCTCTCAGCAGCTACAACATCA TGGGTCCCAGTATGAAGCTCAACACAGACGAGACACCAATGTCTTAGTCTATATCAAAAGAAGGCTGGCG ATGTGTGCCCGGAGACTTGGAAGGACCAGAGAAGCAGTGAAGATGATGAGAGATTTAATGAAGGAGTTTC CCCTCCTAAGCATGTTCAATATCCATGAGAACCTTCTAGAAGCTCTTCTGGAACTCCAAGCTTATGCTGA TGTTCAGGCAGTCTTAGCAAAGTATGATGATATAAGCTTACCAAAGTCGGCGACAATATGCTACACAGCC GCCCTGCTCAAAGCAAGAGCTGTCTCTGACAAATTCTCTCCAGAGGCTGCGTCTCGGAGGGGGCTGAGCA CAGCAGAGATGAATGCAGTAGAAGCCATCCACAGAGCTGTGGAATTTAATCCACACGTGCCAAAATATCT ACTAGAAATGAAAAGCTTAATCCTCCCACCAGAACACATCCTGAAGAGAGGAGACAGTGAAGCGATAGCA TATGCATTCTTTCATCTTGCACACTGGAAGAGGGTGGAAGGGGCTTTGAATCTCTTGCATTGTACGTGGG AAGGCACTTTCCGGATGATCCCGTATCCCCTGGAGAAGGGACACCTATTTTATCCATACCCAATCTGTAC AGAAACAGCTGACCGGGAGCTGCTTCCCTCTTTCCATGAAGTCTCAGTTTACCCAAAGAAGGAACTTCCC TTCTTCATCCTCTTCACTGCTGGACTGTGCTCCTTCACAGCCATGCTGGCCCTCCTGACACATCAGTTTC CGGAACTTATGGGAGTCTTCGCAAAAGCTTTCCTCAGCACTTTGTTTGCCCCCTTGAACTTTGTTATGGA GAAAGTGGAAAGCATCCTCCCCTCCAGTTTGTGGCATCAGCTGACACGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230490 representing NM_001289626 Red=Cloning site Green=Tags(s) MAEAGAGFLEQLKSCIVWSWTYLWTVWFFLVLFLVYILRVPLRINDNLSTVSMFLNTLTPKFYVALTGTS SLISGLILIFEWWYFRKYGTSFIEQVSVSHLRPLLGGVDNNSSNNSNSSNGDSDSNRQSVSECKVWRNPL NLFRGAEYNRYTWVTGREPLTYYDMNLSAQDHQTFFTCDSDHLRPADAIMQKAWRERNPQARISAAHEAL EINECATAYILLAEEEATTIAEAEKLFKQALKAGDGCYRRSQQLQHHGSQYEAQHRRDTNVLVYIKRRLA MCARRLGRTREAVKMMRDLMKEFPLLSMFNIHENLLEALLELQAYADVQAVLAKYDDISLPKSATICYTA ALLKARAVSDKFSPEAASRRGLSTAEMNAVEAIHRAVEFNPHVPKYLLEMKSLILPPEHILKRGDSEAIA YAFFHLAHWKRVEGALNLLHCTWEGTFRMIPYPLEKGHLFYPYPICTETADRELLPSFHEVSVYPKKELP FFILFTAGLCSFTAMLALLTHQFPELMGVFAKAFLSTLFAPLNFVMEKVESILPSSLWHQLTRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289626
ORF Size	1662 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289626.1, NP_001276555.1
RefSeq Size	2141 bp
RefSeq ORF	1665 bp
Locus ID	64213
MW	63.9 kDa

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