Tiam2 (NM_001286758) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	3000002F19Rik; mKIAA2016; STEF
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230499 representing NM_001286758 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCACTTTTTGGGAGCCTGCCAGAGATGCTGGAGTTTCAAAAGGTGTTCCTGGAGACGTTGGAGG ATGCGATCTCCGCTTCCTCGGACTTTAGTGTCCTGGAAACCCCCTCACAGTTTCGAAAATTGCTGTTCTC CCTTGGAGGTTCTTTCCTCTACTATGCGGATCACTTTAAGCTATACAGTGGGTTCTGTGCCAACCACATT AAAGTACAGAGGGTTCTAGAGCGAGCTAAAACGGACAAGGCCTTCAAGGCTTTTCTGGATGCCCGAAATC CCACCAAGCAGCACTCCTCCACGCTGGAGTCCTATCTCATCAAGCCTGTTCAGAGAGTGCTCAAGTATCC TCTGCTTCTCAAGGAGCTAGTGTCACTGACTGACCATGAGAGTGAAGAACACTATCACCTGACAGAAGCA CTAAAGGCCATGGAAAAAGTCGCCAGTCACATCAATGAGATGCAGAAGATCTACGAGGATTACGGGATGG TGTTTGACCAGCTGGTGGCAGAGCAGAGTGGCACAGAGAAGGAAGTGACAGAGCTGTCCATGGGGGAACT TCTGATGCACTCTACAGTTTCCTGGTTGAATCCGTTCCTGTCTCTAGGAAAAGCCAGGAAGGACATTGAG CTCACAGTATTTGTTTTTAAGAGAGCTGTCATACTGGTTTATAAAGAAAACTGCAAGCTGAAAAAGAAAC TGCCCTCGAATTCCCGGCCTGCTCACAACTCTGCTGACTTGGATCCATTTAAATTCCGCTGGTTGATTCC CATATCTGCGCTTCAAGTTAGACTGGGGAACACGGCAGGGACTGAAAATAATTCCACGTGGGAGCTGATT CATACCAAGTCGGAAATTGAAGGACGGCCAGAAACCATCTTTCAACTGTGCTGCAGTGACAGCGAGAACA AAACCAGCATTGTTAAGGTGATTCGTTCTATTCTGAGAGAGAACTTCCGGCGCCACATAAAGTGTGAGCT GCCACTGGAGAAGACGTGTAAGGACCGGCTAGTACCTCTTAAGAACCGAGTTCCTGTTTCAGCCAAATTA GCCTCGTCCAGGTCGTTGAAGGGCCTCAGAACATCCTCCAGCAGCGAGTGGCCCAGCGAGCCCAGCAAGG GCAACTCACTGGACTCAGATGAGTGCAGCCTGAGCAGTGGCACCCAGAGTAGCGGCTGCCCCGTAGCCGA GAGCAGGCGAGACTCTAAGAGCACCGAGCTGGAGAAAGACGCTCAGGAGGGCCTGGCGGAGTTTCCAGAT GGTCTTATCAAAGAAAGCGACATTCTGAGTGATGAAGATGAGGACTTCCACCACCCTCTGAAACAGGGTA GCCCTACTAAGGACATTGAGATTCAGTTCCAGAGACTGAAAATCTCTGAGGAATCCGACGTGCACCCAGT TGGGCAGCAGCCTCTCACAGAGTCAGGTGAACAGCCCAAGCTGGTCAGGGGCCATTTTTGCCCCATTAAA CGGAAAGCAAACAGCACCAAGAGGGGCAGAGGAACTTTGCTCAAGGCGCAGACTCGTCACCAGTCCCTGG ACAGCCACCCAGAAACTGCCAGCATTGATCTAAACTTGGTCCTGGAGAGAGAATTCAGTGTCCAGAGCTT AACTTCAGTCGTCAATGAGGAGGGTTTTTATGAAACACAGAGCCATGGCAAATCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230499 representing NM_001286758 Red=Cloning site Green=Tags(s) MESLFGSLPEMLEFQKVFLETLEDAISASSDFSVLETPSQFRKLLFSLGGSFLYYADHFKLYSGFCANHI KVQRVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLKELVSLTDHESEEHYHLTEA LKAMEKVASHINEMQKIYEDYGMVFDQLVAEQSGTEKEVTELSMGELLMHSTVSWLNPFLSLGKARKDIE LTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSADLDPFKFRWLIPISALQVRLGNTAGTENNSTWELI HTKSEIEGRPETIFQLCCSDSENKTSIVKVIRSILRENFRRHIKCELPLEKTCKDRLVPLKNRVPVSAKL ASSRSLKGLRTSSSSEWPSEPSKGNSLDSDECSLSSGTQSSGCPVAESRRDSKSTELEKDAQEGLAEFPD GLIKESDILSDEDEDFHHPLKQGSPTKDIEIQFQRLKISEESDVHPVGQQPLTESGEQPKLVRGHFCPIK RKANSTKRGRGTLLKAQTRHQSLDSHPETASIDLNLVLEREFSVQSLTSVVNEEGFYETQSHGKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286758
ORF Size	1665 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286758.1, NP_001273687.1
RefSeq Size	2550 bp
RefSeq ORF	1668 bp
Locus ID	24001
UniProt ID	Q6ZPF3
MW	63.1 kDa
Gene Summary	Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Activates specifically RAC1, but not CDC42 and RHOA. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane.[UniProtKB/Swiss-Prot Function]

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