Hs6st2 (NM_001290467) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6OST2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230676 representing NM_001290467 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACTGCCTGCGTTTGCAGCCCGGGCGCTCGGGCCGCCACTGCAACCGGAGCAAGGAGCGCCCGCCC GCACCACCTGTCCCCGCCGGCATTCCAGAGTAGAGGCCGAATTGGCAGCAAGCCGGCCCGGGTCTGTTGC CGCCTCAGTCCGCGCCGGCCCTCCTAGGGGTGTGTCTCTCGGATTCAACTCCCCGCCGCTCCAGGACAAG CCCCCAAAGGCATTTTCTTCCTTGGCGGGAGCCTTGCGCGCCCCGCTCTTCGCGCTGCTGCCTCGAGGCC GCCGCAGGCGGATGCACGACCTCAGGCGACGCTGGGACCTGGGCTCCCTCTGCCGGGCCCTGCTCACTCG GGGCCTGGCAGCCGTGGGCCACTCGCTCAAGCACGTGCTCAGTGCGATCTTCTCCAAGATTTTCGGTCCC TTGGCCAGCGTCGGGAACATGGATGAGAAATCTAACAAGCTGCTGCTGGCTTTGGTGATGCTCTTCCTAT TTGCGGTGATCGTCCTCCAATACGTGTGCCCCGGCACAGAATGCCAGCTCCTCCGCCTGCAGGCGTTCAG CTCCCCGGTGCCGGATCCGTACCGCTCGGAGGATGAGAGTTCGGCCAGGTTTGTACCCCGCTACAATTTC AGCCGCGGCGATCTCCTGCGCAAGGTAGACTTCGACATCAAGGGCGATGACCTGATCGTGTTCCTGCACA TCCAGAAGACTGGGGGCACCACTTTTGGCCGTCACCTGGTGCGCAACATCCAGCTGGAGCAGCCATGTGA GTGCCGCGTGGGGCAGAAGAAATGCACTTGCCACCGGCCGGGTAAGAGGGAGACCTGGCTCTTCTCCAGG TTCTCCACCGGCTGGAGCTGCGGGCTGCATGCCGACTGGACCGAGCTCACCAGCTGCGTGCCGGCGGTGG TGGATGGCAAGCGCGACGCCAGGCTGAGACCTTCCAGGAACTTCCATTACATTACCATCCTGAGAGACCC AGTGTCACGGTACTTGAGTGAATGGAGGCATGTCCAGAGAGGAGCAACTTGGAAAGCATCCCTGCACGTC TGTGATGGAAGGCCCCCAACCTCTGAAGAGCTGCCCAGCTGCTACACCGGTGATGACTGGTCTGGATGCC CTCTCAAAGAGTTCATGGACTGTCCCTATAATCTGGCCAACAACCGCCAAGTTCGCATGCTATCTGACCT GACTCTAGTGGGATGCTACAACCTCTCTGTCATGCCTGAAAAGCAAAGAAACAAAGTCCTTCTGGAAAGT GCCAAATCCAATCTGAAGCACATGGCGTTCTTTGGCCTCACTGAGTTTCAGCGCAAGACCCAGTACCTGT TTGAGAAGACCTTCAACATGAACTTTATCTCGCCGTTTACCCAGTATAATACCACTAGGGCCTCTAGTGT TGAGATCAATGAGGAAATCCAAAAGCGTATTGAGGGACTGAATTTTCTGGATATGGAGTTGTACAGCTAT GCTAAAGACCTCTTTCTGCAAAGGTATCAGTTCATGAGGCAGAAAGAACATCAGGATGCCAGGCGGAAGC GTCAGGAGCAACGCAAATTTCTGAAGGGAAGGTTCCTTCAGACCCATTTCCAGAGTCAGAGTCAGGGTCA GAGCCAGAGCCAGAGTCCAGGTCAGAATCTGAGTCAGAATCCAAATCCTAACCCAAATCAGAACCTGACT CAGAACCTGAGTCACAATCTGACTCCGAGTTCAAATCCCAATTCGACCCAGAGGGAGAACCGGGGAAGTC AGAAGCAGGGCTCAGGCCAGGGACAAGGTGATAGCGGCACCAGCAATGGCACCAATGACTACATAGGGAG CGTAGAGACATGGCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230676 representing NM_001290467 Red=Cloning site Green=Tags(s) MALPAFAARALGPPLQPEQGAPARTTCPRRHSRVEAELAASRPGSVAASVRAGPPRGVSLGFNSPPLQDK PPKAFSSLAGALRAPLFALLPRGRRRRMHDLRRRWDLGSLCRALLTRGLAAVGHSLKHVLSAIFSKIFGP LASVGNMDEKSNKLLLALVMLFLFAVIVLQYVCPGTECQLLRLQAFSSPVPDPYRSEDESSARFVPRYNF SRGDLLRKVDFDIKGDDLIVFLHIQKTGGTTFGRHLVRNIQLEQPCECRVGQKKCTCHRPGKRETWLFSR FSTGWSCGLHADWTELTSCVPAVVDGKRDARLRPSRNFHYITILRDPVSRYLSEWRHVQRGATWKASLHV CDGRPPTSEELPSCYTGDDWSGCPLKEFMDCPYNLANNRQVRMLSDLTLVGCYNLSVMPEKQRNKVLLES AKSNLKHMAFFGLTEFQRKTQYLFEKTFNMNFISPFTQYNTTRASSVEINEEIQKRIEGLNFLDMELYSY AKDLFLQRYQFMRQKEHQDARRKRQEQRKFLKGRFLQTHFQSQSQGQSQSQSPGQNLSQNPNPNPNQNLT QNLSHNLTPSSNPNSTQRENRGSQKQGSGQGQGDSGTSNGTNDYIGSVETWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290467
ORF Size	1836 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290467.1, NP_001277396.1
RefSeq Size	4672 bp
RefSeq ORF	1839 bp
Locus ID	50786
UniProt ID	Q80UW0
MW	69.6 kDa
Gene Summary	6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.[UniProtKB/Swiss-Prot Function]

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