Gga3 (NM_001252067) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C230037M19Rik; mKIAA0154
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230764 representing NM_001252067 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCGGAAGGGGAGAGCCTGGAGTCCTGGCTGAATAAAGCTACCAATCCTTCCAACCGCCAGG AAGACTGGGAATATATCATTGGTTTCTGTGATCAGATCAACAAAGAGCTTGAAGGGCCCCAGATTGCTGT GCGACTCCTGGCCCACAAGATCCAGTCTCCACAGGAATGGGAGGCAGTGCAGGCCCTCACGGTGCTGGAG GCGTGTATGAAGAACTGTGGCAGGAGACTTCATAATGAAGTGGGGAAGTTCCGGTTTTTGAATGAATTAA TCAAAGTCGTCTCTCCAAAGTACTTGGGAGACAGGGTGTCTGAAAAGGTGAAGACTAAGGTCATCGAGTT GCTTTTTAGCTGGACACTGGCCTTGCCGGAAGAAGCAAAGATCAAAGATGCCTACCATATGTTGAAGAGA CAAGGCATAGTGCAGTCGGACCCCCCCATCCCCATGGATAGAACGCTGATCCCCTCTCCCCCACCTCGGC CCAAAAACCCCGTTTTTGATGATGAGGAGAAATCCAAGCTTTTAGCCAGGCTGCTGAAGAGCAAGAACCC AGATGACCTGCAAGAGGCCAACAGACTCATCAAGTCCATGGTGAAGGAAGATGAGGCTCGCATCCAGAAA GTGACCAAGCGTCTACACACACTGGAGGAGGTTAACAACAATGTCAAGCTGCTCCACGAGATGCTGCTTC ACTACAGCCAAGAGTACTCGTCAGACGCCGACAAGGAGCTCATGAAGGAGCTCTTTGATCGTTGTGAGAA TAAGAGGCGGACGCTGTTTAAACTAGCCAGCGAGACAGAGGACAATGACAACAGTTTGGGGGACATCCTG CAGGCCAGTGACAACCTGTCCAGGGTCATCAACTCTTACAAAACTATTATTGAAGGGCAGATCGTCAATG GTGAGGTGACCACCTCAACCATGCCTGACTCTGAAGGCCTTACTGACCCCGCCCCCACTGCTCCCAAAGA GTCACCTGGAAGTAGTCAGTGGCACCTGTTCCAGAATGAACCACCATCAGATCTGGACTTCTTCAGTCCC AGGCCAGTGCCTGCGGCCTCCTGCCCCTCAGATGGACCCCAGCTCCCTCCCCCAGTTTCTACCTCAAGTA TGTCCCAAGCTCCACTGCCTGCTGCCTTCCCAGCTCCTGTGGTCCCAGCCAGTGCACCAACCCACAGCAC TGGCTCCTTCATGTTCTCTTCTGGACCTGCCCCAGCCTTGGCTCCAAAGGCTGAGCCCAAAGGTCCAGAA TACCCCAGCTCCAGCACCTCGCACCGCCTAGATGCCCTTGATCAGCTTCTGGAAGAGGCCAAAGTAACCT CAGGCTTGGTGAAACCTGTTTCTTGCTTCTCTCCTGGGCCCACTGCCTCCCCGCTGCTGCCTGCCTCTGC CCCAGCCAGACCTCTCCTGCCCTTCTCCACTGGGCCCGGAAGCCCTCTCTTCCAGTCCCAAGGCAGCCCT CAGAAAGGCCCAGAGCTCTCCTTGGCCAGTGTCCATGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCC TTCCCGTGACAGCCTATGATAAAAATGGCTTCCGCATCCTTTTCCACTTTGCTAAGGAGTGTCCGCCAGG ACGGCCTGATGTGCTGGTGGTGGTGGTTTCCATGCTGAATACGGCTCCGTTGCCAGTCAAGAGCATAGTG CTGCAGGCTGCAGTGCCCAAGTCAATGAAAGTGAAGTTGCAGCCACCCTCTGGGACAGAACTTTCCCCAT TTAGTCCTATCCAGCCACCTGCAGCCATCACCCAGGTCATGCTGCTGGCCAATCCAATGAAGGAAAAGGT GCGGCTTCGATATAAACTGACCTTTGCTTTGGGGGAGCAGCTGAGCACAGAACTGGGTGAAGTGGACCAG TTTCCTCCTGTGGAGCAGTGGGGGAACCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230764 representing NM_001252067 Red=Cloning site Green=Tags(s) MAEAEGESLESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIAVRLLAHKIQSPQEWEAVQALTVLE ACMKNCGRRLHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLFSWTLALPEEAKIKDAYHMLKR QGIVQSDPPIPMDRTLIPSPPPRPKNPVFDDEEKSKLLARLLKSKNPDDLQEANRLIKSMVKEDEARIQK VTKRLHTLEEVNNNVKLLHEMLLHYSQEYSSDADKELMKELFDRCENKRRTLFKLASETEDNDNSLGDIL QASDNLSRVINSYKTIIEGQIVNGEVTTSTMPDSEGLTDPAPTAPKESPGSSQWHLFQNEPPSDLDFFSP RPVPAASCPSDGPQLPPPVSTSSMSQAPLPAAFPAPVVPASAPTHSTGSFMFSSGPAPALAPKAEPKGPE YPSSSTSHRLDALDQLLEEAKVTSGLVKPVSCFSPGPTASPLLPASAPARPLLPFSTGPGSPLFQSQGSP QKGPELSLASVHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVSMLNTAPLPVKSIV LQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPMKEKVRLRYKLTFALGEQLSTELGEVDQ FPPVEQWGNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252067
ORF Size	1920 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252067.1, NP_001238996.1
RefSeq Size	3469 bp
RefSeq ORF	1923 bp
Locus ID	260302
MW	70.6 kDa
Gene Summary	Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif (By similarity).[UniProtKB/Swiss-Prot Function]

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