Mylk3 (NM_001297612) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	D830007F02Rik; MLCK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230816 representing NM_001297612 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAACTCTGTGCCCTGTCCCAGCACTGTGCAGGCCCAGCACCAGAGGCCAGCAGCGGGCATCAGATT ACACCCAGATATTTGCGAACAAGGAGCAAGCAGAAGTTGCTGGAGTGAAGCCAAACCATGTACTGACTAC AGGAGGTGTGCAAGCTGACGCCTCTAGGACGCTGTGGGAAGAGAGCCAAAAGGAGGACATACCTGTGCGA ACAGTGGAGGGGCTGCCTCTCATCATCAATACGTCACTGAAGGGAGCTGACCTAACCCAGGCAGGAGCCT CACTGAGGCAGGGAGTTGAAGTTCTTGGCCCAGGCCAAGTACCCCTACCCACAGAAGCAGAATCCAGGCT TCCTGAGACAGCCAGTGAGAACACTGGAGCCACCCTGGAATTGTCCGTAGCAATCGACAGAATCAGCGAG GTCCTCACTAGCCTCAAGATGTCACAAGGTGGTGGTCAAGAAACCTCATCCAGCAAGCCTGACTGTTGGC TTTCAGAAGAGGCCATGAGGCTGAGTTCAGGGCCTCTCCCTCAGCCCCTAGGACCACTAACTCCAGACAG TGACATTCACAGTGGTGATGCACTTCCCAGGATCCCTATCAATATGCAAGAGATGGCTACTCCTGGGGAG TTGCTTGAGACCCAAAGTGGCAGTCCCATTGGCTCTGCAGAAGCTCCAGGCCTTGGGACTGTGTTAGAAG ACCAGATCCCTAAAGGAGCCAGACCATTTCCACCCCTGCCAAAGAGGAGCAGCAACAATGGTGGCATGAG TGCAGAGGAGGAGATAGGGTCTGGGGCTGAGCCTATGAGAGGACCAAGCTTGGCTACAAGGGACTGGAGA GATGAGACTGTTGGGACCACAGACCTGCAGCAAGGCATAGACCCAGGAGCAGTGAGCCCTGAGCCTGGGA AGGACCACGCAGCCCAGGGCCCAGGAAGAACTGAAGCTGGAAGGCTATCTTCTGCTGCAGAGGCTGCCAT TGTGGTTCTAGATGACAGCGCAGCACCCCCAGCCCCTTTTGAACACCGGGTAGTGAGCATCAAAGATACC CTGATCTCAGCAGGCTACACGGTATCCCAACATGAAGTCTTAGGAGGGGGTCGGTTTGGCCAGGTGCACA GGTGTACAGAGAGGTCTACAGGCCTTGCACTGGCAGCCAAGATCATCAAAGTGAAGAACGTAAAGGACCG GGAGGATGTGAAGAATGAGGTCAACATCATGAACCAGCTCAGCCACGTAAACTTGATCCAACTTTATGAT GCGTTTGAGAGCAAGAGCAGCTTCACTCTGATCATGGAGTATGTGGATGGAGGCGAACTCTTTGACCGGA TCACGGATGAGAAGTACCACCTCACTGAGTTGGATGTGGTCTTGTTCACGAGGCAGATCTGTGAGGGTGT GCATTACCTGCATCAGCACTATATCCTGCACCTGGACCTCAAGCCTGAGAACATATTGTGTGTCAGCCAG ACAGGGCATCAAATTAAGATCATTGACTTTGGGCTGGCTAGAAGATACAAGCCTCGGGAGAAGCTAAAGG TGAACTTTGGTACTCCGGAGTTCCTGGCCCCAGAAGTTGTTAACTATGAGTTTGTGTCATTTCCAACAGA CATGTGGAGTGTGGGAGTTATCACCTACATGCTACTCAGTGGTTTGTCCCCATTTCTAGGGGAGACAGAT GCAGAGACCATGAATTTTATTGTGAACTGCAGCTGGGATTTCGATGCTGATACCTTCAAAGGGCTGTCGG AGGAAGCCAAGGACTTTGTTTCCCGGTTACTGGTCAAAGAGAAGAGCTGTAGGATGAGCGCCACACAGTG CCTGAAACACGAGTGGTTAAGTCACCTGCCTGCCAAAGCCTCGGGCTCCAACGTTCGCCTCAGATCCCAA CAACTGCTGCAGAAATATATGGCTCAGAGTAAATGGAAGAAACATTTCCACGTGGTGACTGCAGTCAACA GGCTACGGAAATTTCCAACGTGTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230816 representing NM_001297612 Red=Cloning site Green=Tags(s) MGTLCPVPALCRPSTRGQQRASDYTQIFANKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVR TVEGLPLIINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISE VLTSLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGE LLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWR DETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDT LISAGYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYD AFESKSSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQ TGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETD AETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQ QLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001297612
ORF Size	1986 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001297612.1, NP_001284541.1
RefSeq Size	2617 bp
RefSeq ORF	1989 bp
Locus ID	213435
UniProt ID	Q3UIZ8
MW	72.6 kDa
Gene Summary	Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.[UniProtKB/Swiss-Prot Function]

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