Mylk3 (NM_001297612) Mouse Tagged ORF Clone
CAT#: MR230816
- TrueORF®
Mylk3 (myc-DDK-tagged) - Mouse myosin light chain kinase 3 (Mylk3), transcript variant 2
"NM_001297612" in other vectors (1)
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CNY 6,650.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D830007F02Rik; MLCK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230816 representing NM_001297612
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAACTCTGTGCCCTGTCCCAGCACTGTGCAGGCCCAGCACCAGAGGCCAGCAGCGGGCATCAGATT ACACCCAGATATTTGCGAACAAGGAGCAAGCAGAAGTTGCTGGAGTGAAGCCAAACCATGTACTGACTAC AGGAGGTGTGCAAGCTGACGCCTCTAGGACGCTGTGGGAAGAGAGCCAAAAGGAGGACATACCTGTGCGA ACAGTGGAGGGGCTGCCTCTCATCATCAATACGTCACTGAAGGGAGCTGACCTAACCCAGGCAGGAGCCT CACTGAGGCAGGGAGTTGAAGTTCTTGGCCCAGGCCAAGTACCCCTACCCACAGAAGCAGAATCCAGGCT TCCTGAGACAGCCAGTGAGAACACTGGAGCCACCCTGGAATTGTCCGTAGCAATCGACAGAATCAGCGAG GTCCTCACTAGCCTCAAGATGTCACAAGGTGGTGGTCAAGAAACCTCATCCAGCAAGCCTGACTGTTGGC TTTCAGAAGAGGCCATGAGGCTGAGTTCAGGGCCTCTCCCTCAGCCCCTAGGACCACTAACTCCAGACAG TGACATTCACAGTGGTGATGCACTTCCCAGGATCCCTATCAATATGCAAGAGATGGCTACTCCTGGGGAG TTGCTTGAGACCCAAAGTGGCAGTCCCATTGGCTCTGCAGAAGCTCCAGGCCTTGGGACTGTGTTAGAAG ACCAGATCCCTAAAGGAGCCAGACCATTTCCACCCCTGCCAAAGAGGAGCAGCAACAATGGTGGCATGAG TGCAGAGGAGGAGATAGGGTCTGGGGCTGAGCCTATGAGAGGACCAAGCTTGGCTACAAGGGACTGGAGA GATGAGACTGTTGGGACCACAGACCTGCAGCAAGGCATAGACCCAGGAGCAGTGAGCCCTGAGCCTGGGA AGGACCACGCAGCCCAGGGCCCAGGAAGAACTGAAGCTGGAAGGCTATCTTCTGCTGCAGAGGCTGCCAT TGTGGTTCTAGATGACAGCGCAGCACCCCCAGCCCCTTTTGAACACCGGGTAGTGAGCATCAAAGATACC CTGATCTCAGCAGGCTACACGGTATCCCAACATGAAGTCTTAGGAGGGGGTCGGTTTGGCCAGGTGCACA GGTGTACAGAGAGGTCTACAGGCCTTGCACTGGCAGCCAAGATCATCAAAGTGAAGAACGTAAAGGACCG GGAGGATGTGAAGAATGAGGTCAACATCATGAACCAGCTCAGCCACGTAAACTTGATCCAACTTTATGAT GCGTTTGAGAGCAAGAGCAGCTTCACTCTGATCATGGAGTATGTGGATGGAGGCGAACTCTTTGACCGGA TCACGGATGAGAAGTACCACCTCACTGAGTTGGATGTGGTCTTGTTCACGAGGCAGATCTGTGAGGGTGT GCATTACCTGCATCAGCACTATATCCTGCACCTGGACCTCAAGCCTGAGAACATATTGTGTGTCAGCCAG ACAGGGCATCAAATTAAGATCATTGACTTTGGGCTGGCTAGAAGATACAAGCCTCGGGAGAAGCTAAAGG TGAACTTTGGTACTCCGGAGTTCCTGGCCCCAGAAGTTGTTAACTATGAGTTTGTGTCATTTCCAACAGA CATGTGGAGTGTGGGAGTTATCACCTACATGCTACTCAGTGGTTTGTCCCCATTTCTAGGGGAGACAGAT GCAGAGACCATGAATTTTATTGTGAACTGCAGCTGGGATTTCGATGCTGATACCTTCAAAGGGCTGTCGG AGGAAGCCAAGGACTTTGTTTCCCGGTTACTGGTCAAAGAGAAGAGCTGTAGGATGAGCGCCACACAGTG CCTGAAACACGAGTGGTTAAGTCACCTGCCTGCCAAAGCCTCGGGCTCCAACGTTCGCCTCAGATCCCAA CAACTGCTGCAGAAATATATGGCTCAGAGTAAATGGAAGAAACATTTCCACGTGGTGACTGCAGTCAACA GGCTACGGAAATTTCCAACGTGTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230816 representing NM_001297612
Red=Cloning site Green=Tags(s) MGTLCPVPALCRPSTRGQQRASDYTQIFANKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVR TVEGLPLIINTSLKGADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISE VLTSLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPINMQEMATPGE LLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAEEEIGSGAEPMRGPSLATRDWR DETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAGRLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDT LISAGYTVSQHEVLGGGRFGQVHRCTERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYD AFESKSSFTLIMEYVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQ TGHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLLSGLSPFLGETD AETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQCLKHEWLSHLPAKASGSNVRLRSQ QLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001297612 |
ORF Size | 1986 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001297612.1, NP_001284541.1 |
RefSeq Size | 2617 bp |
RefSeq ORF | 1989 bp |
Locus ID | 213435 |
UniProt ID | Q3UIZ8 |
MW | 72.6 kDa |
Gene Summary | Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228605 | Mylk3 (untagged) - Mouse myosin light chain kinase 3 (Mylk3), transcript variant 2 |
CNY 6,460.00 |