Ppp2r1b (NM_001286553) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2410091N08Rik; AI790395
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230908 representing NM_001286553 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGCAGCAGGGCCAGGGTCCGGTCCGGGGGCAGCGGGTGGAGATGGAGACGATTCGCTCTACC CGATCGCGGTTTTAATCGACGAGCTCCGCAATGAGGACGTGCAGCTCCGTCTCAACAGTATTAAGAAGTT ATCAACTATAGCTCTAGCACTCGGGGTAGAAAGGACTCGAACGGAACTGCTGCCTTTCCTTACAGATACT ATTTATGATGAAGATGAGGTGCTGTTAGCTCTCGCCGAGCAGCTGGGAAATTTCACTGGTTTGGTGGGCG GTCCTGACTTTGCCCACTGTCTGCTGCCTCCTTTGGAAAGTTTGGCTACGGTGGAGGAGACTGTCGTTCG AGACAAGGCTGTGGAGTCGCTGAGGCAGATCTCGCAGGAGCACACTCCTGTTGCCCTTGAAGCTCATTTT GTGCCTCTGGTGAAGCGCCTGGCAAGCGGGGACTGGTTCACGTCGCGCACGTCTGCATGTGGCTTGTTCA GCGTTTGCTATCCCAGAGCTTCAAATGCTGTCAAGGCAGAGATTCGACAGCACTTCCGTTCTCTCTGCTC GGATGATACACCAATGGTGCGACGTGCTGCTGCTTCCAAATTGGGTGAATTTGCAAAAGTTTTGGAATTA GACAGTGTGAAAACTGAAATTGTCCCATTGTTCACGAATTTGGCTTCAGATGAACAGGACTCTGTGCGGC TGCTAGCTGTGGAAGCCTGCGTCAGCATTGCCCAGTTACTGTCTCAGGAGGACCTGGAAGCCTTGGTAAT GCCTACACTTCGACAAGCAGCAGAAGATAAGTCCTGGCGGGTTCGATATATGGTGGCTGACAAGTTTTCA GAGCTCCAGAAAGCGGTGGGCCCTAAGATTGCACTCAGCGACCTCATCCCCGCCTTCCAGAGCCTCCTCC GAGACTGCGAAGCTGAAGTCCGAGCAGCCGCTGCCCACAAAGTAAGAGAGCTTTGTGAGAACCTGCCCGC TGAAGGTAGAGAGACTGTAATTATGAATCAAATCCTGCCCTATATAAAGGAATTAGTGTCTGATACCAAC CAACATGTCAAGTCAGCGCTAGCTTCTGTAATTATGGGATTATCTACAGTTTTGGGCAAAGAGAATACCA TTGAACATCTTCTGCCTCTATTTTTAGCTCAGTTAAAGGACGAGTGTCCAGAAGTTCGTTTAAATATCAT CTCCAATTTGGATTGTGTGAATGAAGTGATTGGAATCCGTCAGCTCTCTCAGTCTCTCCTTCCGGCCATA GTGGAACTGGCTGAAGATGCCAAGTGGAGGGTCCGCCTGGCTATCATTGAGTACATGCCGCTGCTGGCTG GCCAGCTGGGTGTGGAGTTCTTTGATGAAAAACTCAATTCTTTGTGTATGGCCTGGCTTGTGGATCATGT CTATGCCATTCGGGAGGCAGCCACCAACAACCTCATGAAGTTAGTCCAGAAGTTCGGTACAGAGTGGGCC CAGAATACCATTGTCCCTAAAGTGTTGGTAATGGCAAATGACCCTAACTACTTACACAGAATGACCACGC TCTTCTGCATTAATGCACTGTCTGAAGCCTGTGGTAAGGAAATAACCACTAAGCAAATGTTGCCCATCGT GTTGAAAATGGCAGGAGACCAGGTAGCAAATGTCCGCTTCAATGTGGCCAAATCACTGCAGAAAATTGGA CCGATTCTAGACACCAACGCCTTGCAGGGGGAAGTGAAGCCGGTGCTGCAGAAATTGGGTCAGGACGAAG ACATGGATGTCAAATATTTTGCACAGGAAGCTATAAGTGTGGTGGCACAAAGGCTGAGGAAGCTAGATTT TCCTGTGAAGGACAGTGAAGAGCCCAGCATCCCTGGGGTTGACAAGAATCTCTTCCTGAGACCCCGAGGG CCTGGAGAGGACACTGGGAAGGCACAGAGAGACGGAGCGGCTCCAGTGGCTTCCGAGGAGGCAGTAGAGG CAGCCCAGCTTAGGGGCACAAACACAGCAAAGGGACCAGCATGTCAGTTGCATATAAATACTAGAGACAC ACTTGCCCAACTGGAAATTGCAGAAGCAATGCATTTCCCCCAAAGCAGAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230908 representing NM_001286553 Red=Cloning site Green=Tags(s) MAGAAGPGSGPGAAGGDGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRTELLPFLTDT IYDEDEVLLALAEQLGNFTGLVGGPDFAHCLLPPLESLATVEETVVRDKAVESLRQISQEHTPVALEAHF VPLVKRLASGDWFTSRTSACGLFSVCYPRASNAVKAEIRQHFRSLCSDDTPMVRRAAASKLGEFAKVLEL DSVKTEIVPLFTNLASDEQDSVRLLAVEACVSIAQLLSQEDLEALVMPTLRQAAEDKSWRVRYMVADKFS ELQKAVGPKIALSDLIPAFQSLLRDCEAEVRAAAAHKVRELCENLPAEGRETVIMNQILPYIKELVSDTN QHVKSALASVIMGLSTVLGKENTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAI VELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKFGTEWA QNTIVPKVLVMANDPNYLHRMTTLFCINALSEACGKEITTKQMLPIVLKMAGDQVANVRFNVAKSLQKIG PILDTNALQGEVKPVLQKLGQDEDMDVKYFAQEAISVVAQRLRKLDFPVKDSEEPSIPGVDKNLFLRPRG PGEDTGKAQRDGAAPVASEEAVEAAQLRGTNTAKGPACQLHINTRDTLAQLEIAEAMHFPQSRE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286553
ORF Size	2082 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286553.1, NP_001273482.1
RefSeq Size	3627 bp
RefSeq ORF	2085 bp
Locus ID	73699
MW	76.5 kDa
Gene Summary	The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.[UniProtKB/Swiss-Prot Function]

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