Add3 (NM_001277100) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI463285; R75380
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230943 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCGACACCAGCCCAGCTGTGGTTACCACGCCTCCTCCTCCCAGCATGCCTCACAAAGAGAGGT ATTTTGACCGAATCAATGAAAGTGATCCGGAATACCTTCGAGAGAGGAACATGTCTCCTGATCTACGACA GGACTTCAATATGATGGAGCAGAGGAAGCGAGTCACTCAGATCCTGCAGAGCCCTGCCTTTCGGGAAGAC CTGGAATGCCTCATCCAAGAGCAGATGAAGAAAGGCCACAACCCAAGCGGCTTATTAGCGTTACAGCAGA TTGCGGATTACATCGTGACCAGCTCCTTCTCGGGCTTCTCCTCGCCCTCCCTGAGTCTTGGCATGGTCAC ACCTATTAATGACCTTCCTGGTGCAGATACATCCTCGTATGTGAAGGGAGAAAAGCTTACTCGCTGCAAA CTTGCCAGCCTCTACAGGCTTGCAGACTTGTTTGGGTGGGCGCACCTGGCAAATACGTACATCTCGGTAC GAATAAGTAAGGAGCAAGACCACATTATAATAATTCCCAGAGGCCTCTCTTTTTCTGAAGCGACAGCCTC CACTTTGGTGAAAGTCAACATAATCGGAGAAGTGGTGGACCAGGGGAGCACTGATCTGAAAATCGACCAC ACGGGCTTCAGCCCGCATGCTGCCATCTATTCAACACGCCCCGATGTGAAGTGTGTGATCCACATCCACA CCCTTGCCACAGCAGCGGTGTCCTCCATGAAGTGTGGGATCCTTCCAATTTCCCAAGAGTCTCTCATCCT GGGAGATGTCGCCTACTATGACTACCAGGGGTCGCTTGATGAGGAGGAAGAAAGAATTGAACTTCAGAAA GTGCTGGGTCCAAGCTGTAAGGTGCTCGTCCTCAGGAACCATGGCATGGTGGCTCTTGGCGAGACCCTCG AGGAGGCTTTCCATTACATCTTTAATGTACAGATGGCCTGTGAGATCCAGGTGCAGGCAGTAGCAGGGGC AGGCGGAGTAGACAATCTGCTCGTACTGGATCTTCAGAAATATAAAGCTTTCACCCACGGTGTCGCCATG TCTGGAGGAGGCGGTGTAAACATGGCTTCCCATCAGAAGTGGAAGGTTGGCGAGATTGAGTTTGAAGGGC TGATGAGGACCCTGGACAACTTGGGCTACAGAACAGGCTATGCGTACAGGCATCCTCTGGTCCGAGAGAA GCCTAGGCACAAGAGTGACGTGGAGATCCCTGCAACCGTGACTGCCTTCTCCTTTGAAGATGACAGTGCT CCACTCTCTCCCCTCAAGTTCATGGCACAGAGACAGCAGCGTGAAAAAACAAGATGGCTGAATTCACCAA ATACTTACATGAAGGTGAATGTGCCGGAGGAGTCTCGGAACGGGGAAACTAGTCCCAGGACCAAAATCAC GTGGATGAAAGCAGAAGACTCCTCTAAAGTCAGTAGTGGGACACCGATCAAAATTGAAGACCCCAATCAG TTTGTTCCTTTAAACACAAACCCGACGGAGGTGCTAGAAAAGAGAAATAAGATCCGGGAACAGAATCGCT ATGATCTGAAGACAGCAGGGCCGCAGTCTCAGCTGCTGGCGGGGATCGTTGTGGACAAGCCGCCTTCGAC CATGCAGTTTGACGATGACGATCAGGGCCCACCAGCTCCTCCTAACCCATTCAGCCACCTCCTGGAAGGA GAACTGGAAGAGTATACGAAGACAATCGAGAGAAAGCAGCAAGGCCTGGACGACGCTGAGCAGGGGTCAC TCTCAGACGACGCTGCATCTGTTTCACAAATTCAGTCTCAAACTCAGTCACCGCAGAGTGTCCCTGAGAG ATTAGAAGAAAACCACGAGCTGTTTTCCAAGAGCTTCACCTCCATGGACGCGCCTGTCATGATCATGAAC GGCAAGGACGAGATGCATGATGTCGAAGACGAGCTTGCTCAGCGAGTCAGTAGGCTGACCACAAGCACGA CCATAGAGAACATCGAGATCACCATTAAGTCCCCAGAAAGAACTGAAGAGGTCCTGTCGCCTGATGGCTC ACCTTCAAAGTCGCCCTCCAAGAAAAAGAAGAAATTCCGCACGCCTTCTTTTCTCAAAAAGAACAAAAAA AAGGAGAAAGTTGAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230943 protein sequence Red=Cloning site Green=Tags(s) MSSDTSPAVVTTPPPPSMPHKERYFDRINESDPEYLRERNMSPDLRQDFNMMEQRKRVTQILQSPAFRED LECLIQEQMKKGHNPSGLLALQQIADYIVTSSFSGFSSPSLSLGMVTPINDLPGADTSSYVKGEKLTRCK LASLYRLADLFGWAHLANTYISVRISKEQDHIIIIPRGLSFSEATASTLVKVNIIGEVVDQGSTDLKIDH TGFSPHAAIYSTRPDVKCVIHIHTLATAAVSSMKCGILPISQESLILGDVAYYDYQGSLDEEEERIELQK VLGPSCKVLVLRNHGMVALGETLEEAFHYIFNVQMACEIQVQAVAGAGGVDNLLVLDLQKYKAFTHGVAM SGGGGVNMASHQKWKVGEIEFEGLMRTLDNLGYRTGYAYRHPLVREKPRHKSDVEIPATVTAFSFEDDSA PLSPLKFMAQRQQREKTRWLNSPNTYMKVNVPEESRNGETSPRTKITWMKAEDSSKVSSGTPIKIEDPNQ FVPLNTNPTEVLEKRNKIREQNRYDLKTAGPQSQLLAGIVVDKPPSTMQFDDDDQGPPAPPNPFSHLLEG ELEEYTKTIERKQQGLDDAEQGSLSDDAASVSQIQSQTQSPQSVPERLEENHELFSKSFTSMDAPVMIMN GKDEMHDVEDELAQRVSRLTTSTTIENIEITIKSPERTEEVLSPDGSPSKSPSKKKKKFRTPSFLKKNKK KEKVEA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277100
ORF Size	2121 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277100.1, NP_001264029.1
RefSeq Size	4511 bp
RefSeq ORF	2121 bp
Locus ID	27360
UniProt ID	Q9QYB5
MW	78.8 kDa
Gene Summary	Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Plays a role in actin filament capping. Binds to calmodulin.[UniProtKB/Swiss-Prot Function]

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