Hgf (NM_001289458) Mouse Tagged ORF Clone
CAT#: MR231001
- TrueORF®
Hgf (myc-DDK-tagged) - Mouse hepatocyte growth factor (Hgf), transcript variant 1
"NM_001289458" in other vectors (1)
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CNY 5,336.00
CNY 7,220.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C230052L06Rik; HGF/S; HGF/SF; NK; NK1; NK2; SF; SF/HG; SF/HGF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231001 representing NM_001289458
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGGGGGACCAAACTTCTGCCGGTCCTGTTGCTGCAGCATGTCCTCCTGCACCTCCTCCTGCTTC ATGTCGCCATCCCCTATGCAGAAGGACAGAAGAAAAGAAGAAATACACTTCATGAATTTAAAAAGTCAGC AAAAACTACTCTTACCAAGGAAGACCCATTACTGAAGATTAAAACCAAAAAAGTGAACTCTGCAGATGAG TGTGCCAACAGGTGTATCAGGAACAGGGGCTTTACGTTCACTTGCAAGGCCTTCGTTTTTGATAAGTCAA GAAAACGATGCTACTGGTATCCTTTCAATAGTATGTCAAGTGGAGTGAAAAAAGGGTTTGGCCATGAATT TGACCTCTATGAAAACAAAGACTATATTAGAAACTGCATCATTGGTAAAGGAGGCAGCTATAAAGGGACG GTATCCATCACTAAGAGTGGCATCAAATGCCAGCCTTGGAATTCCATGATCCCCCATGAACACAGCTTTT TGCCTTCGAGCTATCGCGGTAAAGACCTACAGGAAAACTACTGTCGAAATCCTCGAGGGGAAGAAGGGGG ACCCTGGTGTTTCACAAGCAATCCAGAGGTACGCTACGAAGTCTGTGACATTCCTCAGTGTTCAGAAGTT GAATGCATGACCTGCAATGGTGAAAGCTACAGAGGTCCCATGGATCACACAGAATCAGGCAAGACTTGTC AGCGCTGGGACCAGCAGACACCACACCGGCACAAGTTCTTGCCAGAAAGATATCCCGACAAGGGCTTTGA TGATAATTATTGCCGCAATCCTGATGGCAAGCCGAGGCCATGGTGCTACACTCTTGACCCTGACACCCCT TGGGAGTATTGTGCAATTAAAACGTGCGCTCACAGTGCTGTGAATGAGACTGATGTCCCTATGGAAACAA CTGAATGCATTCAAGGCCAAGGAGAAGGTTACAGGGGAACCAGCAATACCATTTGGAATGGAATTCCCTG TCAGCGTTGGGATTCGCAGTACCCTCACAAGCATGATATCACTCCCGAGAACTTCAAATGCAAGGACCTT AGAGAAAATTATTGCCGCAATCCAGATGGGGCTGAATCACCATGGTGTTTTACCACTGACCCAAACATCC GAGTTGGCTACTGCTCTCAAATTCCCAAGTGTGACGTGTCAAGTGGACAAGATTGTTATCGTGGCAATGG GAAAAATTACATGGGCAACTTATCCAAAACAAGGTCTGGACTTACATGTTCCATGTGGGACAAGAATATG GAGGATTTACACCGTCATATCTTCTGGGAGCCAGATGCTAGCAAATTGAATAAGAATTACTGCCGGAATC CTGATGATGATGCCCATGGACCTTGGTGCTACACGGGGAATCCTCTTATTCCTTGGGATTATTGCCCTAT TTCCCGTTGTGAAGGAGATACTACACCTACAATTGTCAATTTGGACCATCCTGTAATATCCTGTGCCAAA ACAAAACAACTGCGGGTTGTAAATGGCATTCCAACACAAACAACAGTAGGGTGGATGGTTAGTTTGAAAT ACAGAAATAAACATATCTGTGGAGGATCATTGATAAAGGAAAGTTGGGTTCTTACTGCAAGACAATGTTT TCCAGCCAGAAACAAAGACTTGAAAGACTATGAAGCTTGGCTTGGCATCCACGATGTTCATGAGAGAGGC GAGGAGAAGCGCAAGCAGATCTTAAACATTTCCCAGCTGGTCTATGGTCCTGAAGGCTCAGACTTGGTTT TACTGAAGCTTGCTCGACCTGCAATCCTGGATAACTTTGTCAGTACAATTGATTTACCTAGTTATGGTTG TACAATCCCTGAAAAGACCACTTGCAGTATTTACGGCTGGGGCTACACTGGATTGATCAACGCGGATGGT TTATTACGAGTAGCTCATCTGTATATTATGGGGAATGAGAAATGCAGTCAGCACCATCAAGGCAAGGTGA CTTTGAATGAGTCTGAGTTATGTGCTGGGGCTGAAAAGATTGGATCAGGACCATGTGAGGGAGATTATGG TGGCCCACTCATTTGTGAACAACACAAAATGAGAATGGTTCTTGGTGTCATTGTTCCTGGTCGTGGATGT GCCATCCCAAATCGTCCTGGTATTTTTGTTCGAGTAGCATATTATGCAAAATGGATACACAAAGTAATTT TGACATACAAGTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231001 representing NM_001289458
Red=Cloning site Green=Tags(s) MMWGTKLLPVLLLQHVLLHLLLLHVAIPYAEGQKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKKVNSADE CANRCIRNRGFTFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGT VSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEV ECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTP WEYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHDITPENFKCKDL RENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNM EDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAK TKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERG EEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADG LLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGC AIPNRPGIFVRVAYYAKWIHKVILTYKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001289458 |
ORF Size | 2184 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001289458.1, NP_001276387.1 |
RefSeq Size | 2838 bp |
RefSeq ORF | 2187 bp |
Locus ID | 15234 |
UniProt ID | Q08048 |
MW | 82.9 kDa |
Gene Summary | This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Homozygous knockout mice for this gene exhibit embryonic lethality due to impaired development of the placenta and liver. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228790 | Hgf (untagged) - Mouse hepatocyte growth factor (Hgf), transcript variant 1 |
CNY 5,344.00 |