Csf3r (NM_001252651) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Cd114; Csfgr; G-CSF-R; G-CSFR
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231007 representing NM_001252651 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGGGCTGGGAGCCTGCACCCTGACTGGAGTTACCCTGATCTTCTTGCTACTCCCCAGAAGTCTGG AGAGCTGTGGACACATCGAGATTTCACCCCCTGTTGTCCGCCTGGGGGACCCTGTCCTGGCCTCTTGCAC CATCAGCCCAAACTGCAGCAAACTGGACCAACAGGCAAAGATCTTATGGAGACTGCAAGATGAGCCCATC CAACCTGGGGACAGACAGCATCATCTGCCTGATGGGACCCAAGAGTCCCTCATCACTCTGCCTCACTTGA ACTACACCCAGGCCTTCCTCTTCTGCTTAGTGCCATGGGAAGACAGCGTCCAACTCCTGGATCAAGCTGA GCTTCACGCAGGCTATCCCCCTGCCAGCCCCTCAAACCTATCCTGCCTCATGCACCTCACCACCAACAGC CTGGTCTGCCAGTGGGAGCCAGGTCCTGAGACCCACCTGCCCACCAGCTTCATCCTAAAGAGCTTCAGGA GCCGCGCCGACTGTCAGTACCAAGGGGACACCATCCCGGATTGTGTGGCAAAGAAGAGGCAGAACAACTG CTCCATCCCCCGAAAAAACTTGCTCCTGTACCAGTATATGGCCATCTGGGTGCAAGCAGAGAATATGCTA GGGTCCAGCGAGTCCCCAAAGCTGTGCCTCGACCCCATGGATGTTGCCCCCACCATCAGACTGGACACGT GGTGTCAGAAGAAGCAACTAGATCCAGGGACAGTGAGTGTGCAGCTGTTCTGGAAGCCAACGCCCCTGCA GGAAGACAGTGGACAGATCCAGGGCTACCTGCTGTCCTGGAGTTCCCCAGATCATCAAGGGCAGGACATA CACCTTTGCAACACCACGCAGCTCAGCTGTATCTTCCTCCTGCCCTCAGAGGCCCAGAACGTGACCCTTG TGGCCTACAACAAAGCAGGGACCTCTTCACCTACTACAGTGGTTTTCCTGGAGAACGAAGGTCCAGCTGT GACCGGACTCCATGCCATGGCCCAAGACCTTAACACCATCTGGGTAGACTGGGAAGCCCCCAGCCTTCTG CCTCAGGGCTATCTCATTGAGTGGGAAATGAGTTCTCCCAGCTACAATAACAGCTATAAGTCCTGGATGA TAGAACCTAACGGGAACATCACTGGAATTCTGTTAAAGGACAACATAAATCCCTTTCAGCTCTACAGAAT TACAGTGGCTCCCCTGTACCCAGGCATCGTGGGACCTCCTGTAAATGTCTACACCTTCGCTGGAGAGAGA GCTCCTCCTCATGCTCCAGCGCTGCATCTAAAGCATGTTGGCACAACCTGGGCACAGCTGGAGTGGGTAC CTGAGGCCCCTAGGCTGGGGATGATACCCCTCACCCACTACACCATCTTCTGGGCCGATGCTGGGGACCA CTCCTTCTCCGTCACCCTGAACATCTCCCTCCATGACTTTGTCCTGAAGCACCTGGAGCCCGCCAGTTTG TATCATGTCTACCTCATGGCCACCAGTCGGGCAGGGTCCACCAACAGTACAGGCCTTACCCTGAGGACCC TAGATCCATCTGACTTAAACATTTTCCTGGGCATACTTTGCTTAGTACTCTTGTCCACTACCTGTGTAGT GACCTGGCTCTGCTGCAAACGCAGAGGAAAGACTTCCTTCTGGTCAGATGTGCCAGACCCAGCCCACAGT AGCCTGAGCTCCTGGTTGCCCACCATCATGACAGAGGAAACCTTCCAGTTACCCAGCTTCTGGGACTCCA GCGTGCCATCAATCACCAAGATCACTGAACTGGAGGAAGACAAGAAACCGACCCACTGGGATTCCGAAAG CTCTGGGAATGGTAGCCTTCCAGCCCTGGTTCAGGCCTATGTGCTCCAAGGAGATCCAAGAGAAATTTCC AACCAGTCCCAGCCTCCCTCTCGCACTGGTGACCAGGTCCTCTATGGTCAGGTGCTTGAGAGCCCCACCA GCCCAGGAGTAATGCAGTACATTCGCTCTGACTCCACTCAGCCCCTCTTGGGGGGCCCCACCCCTAGCCC TAAATCTTATGAAAACATCTGGTTCCATTCAAGACCCCAGGAGACCTTTGTGCCCCAACCTCCAAACCAG GAAGATGACTGTGTCTTTGGGCCTCCATTTGATTTTCCCCTCTTTCAGGGGCTCCAGGTCCATGGAGTTG AAGAACAAGGGGGTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231007 representing NM_001252651 Red=Cloning site Green=Tags(s) MVGLGACTLTGVTLIFLLLPRSLESCGHIEISPPVVRLGDPVLASCTISPNCSKLDQQAKILWRLQDEPI QPGDRQHHLPDGTQESLITLPHLNYTQAFLFCLVPWEDSVQLLDQAELHAGYPPASPSNLSCLMHLTTNS LVCQWEPGPETHLPTSFILKSFRSRADCQYQGDTIPDCVAKKRQNNCSIPRKNLLLYQYMAIWVQAENML GSSESPKLCLDPMDVAPTIRLDTWCQKKQLDPGTVSVQLFWKPTPLQEDSGQIQGYLLSWSSPDHQGQDI HLCNTTQLSCIFLLPSEAQNVTLVAYNKAGTSSPTTVVFLENEGPAVTGLHAMAQDLNTIWVDWEAPSLL PQGYLIEWEMSSPSYNNSYKSWMIEPNGNITGILLKDNINPFQLYRITVAPLYPGIVGPPVNVYTFAGER APPHAPALHLKHVGTTWAQLEWVPEAPRLGMIPLTHYTIFWADAGDHSFSVTLNISLHDFVLKHLEPASL YHVYLMATSRAGSTNSTGLTLRTLDPSDLNIFLGILCLVLLSTTCVVTWLCCKRRGKTSFWSDVPDPAHS SLSSWLPTIMTEETFQLPSFWDSSVPSITKITELEEDKKPTHWDSESSGNGSLPALVQAYVLQGDPREIS NQSQPPSRTGDQVLYGQVLESPTSPGVMQYIRSDSTQPLLGGPTPSPKSYENIWFHSRPQETFVPQPPNQ EDDCVFGPPFDFPLFQGLQVHGVEEQGGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252651
ORF Size	2187 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252651.1, NP_001239580.1
RefSeq Size	3583 bp
RefSeq ORF	2190 bp
Locus ID	12986
MW	81.3 kDa
Gene Summary	Receptor for granulocyte colony-stimulating factor (CSF3). In addition it may function in some adhesion or recognition events at the cell surface.[UniProtKB/Swiss-Prot Function]

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