Ablim1 (NM_001290813) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2210411C18Rik; 2610209L21Rik; 4833406P10Rik; 9330196J19Rik; AV079770; AW060987
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231009 representing NM_001290813 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATCCTTGCTTGGGTTAAAGTGTCTGGGGAAATTGTGCAGCTCTGAGATAGGCAAAGTTCCCTCAC CAGAGAGAGCCAGCCTCAGGAACTCCCACAGGAGACTGCTTATTGAGGACCTGAGTGTCCCCGAGACTCC TGACCCTGCTCATCGCAGGCGTGGCACAGTCATACATCTGGTCTATCTGTATTCTGCTGGCTGTGGTCCA CCAGAGCTCAGGTTTAGCAGCTATGATCCATCCGTGGCCCATCCTCAGGACCCTCACCACTCGTCGGAGA AACCTGTCATTCACTGCCATAAATGCGGGGAGCCATGCAAGGGCGAAGTGCTACGGGTGCAGACCAAGCA TTTCCACATCAAATGCTTCACCTGCAAAGTGTGCGGCTGCGACCTGGCCCAGGGGGGCTTCTTCATAAAG AACGGGGACTATCTGTGTACCCTGGACTACCAGAGGATGTATGGGACACGGTGCCACGGCTGTGGGGAGT TCGTGGAGGGCGAGGTGGTAACTGCCCTGGGCAAGACCTACCACCCCAACTGCTTCGCCTGTACAATCTG CAAGCGCCCATTTCCACCCGGAGACCGAGTCACATTCAACGGGAGAGACTGTCTTTGTCAGCTCTGTGCA CAGCCGATGTCTTCCAGCCCTAAAGAAGCCTCCTGCTCCAGCAACTGCGCCGGCTGTGGAAGGGACATTA AGAACGGACAGGCGCTGCTAGCTCTGGACAAGCAGTGGCATTTGGGCTGCTTCAAGTGCAAGTCCTGTGG GAAGGTCCTCACCGGCGAGTACATCAGCAAGGATGGCTCCCCATACTGTGAGAAAGACTACCAGGGGCTC TTTGGGGTGAAGTGTGAAGCCTGTCACCAGTTTATCACAGGAAAAGTCCTGGAGGCAGGCGACAAGCATT ATCATCCCAGCTGCGCACGATGCAGCAGATGCAATCAGATGTTCACAGAAGGGGAAGAAATGTATCTGCA AGGTTCTACCGTGTGGCATCCCGACTGTAAGCAGTCCACTAAGACAGAGGAGAAGCTGCGGCCCACCAGG ACCTCCTCTGAAAGTATCTATTCTAGACCAGGCTCCAGCATCCCTGGTTCACCAGGCCATACTATCTATG CAAAAGTAGACAATGAAATCCTGGATTACAAGGATTTAGCAGCCATCCCCAAGGTCAAGGCGATCTATGA CATCGAGCGTCCAGATCTCATTACCTATGAGCCTTTCTACACATCAGGCTATGAGGACAAGCAGGAGAGA CAGAGCCTTGGAGAGTCTCCAAGGACTTTGTCTCCAACTCCATCTGCAGAAGGTTATCAAGATGTCCGGG ATCGGATGATCCACAGGTCCACCAGCCAGGGCTCCATCAACTCCCCTGTGTATAGCCGGCACAGTTACAC TCCAACTACGTCTCGCTCGCCCCAGCACTTCCACCGACCTGGCAATGAGCCGTCCAGCGGCCGGAACTCC CCTCTCCCCTACCGGCCCGACAGCCGCCCTCTAACTCCAACTTACGCTCAGGCCCCTAAACATTTCCATG TTCCAGATCAAGGGATCAACATTTACCGAAAACCACCCATCTACAAACAGCATGGAACTGACCCGAGGCG CAGATCAAGTGGCAGAGAGGAAGATGAAGAGGAGCTTTTGAGACGCCGGCAGCTTCAAGAAGAACAGTTG ATGAAGCTCAACTCAGGCCTGGGGCAGCTGATCCTGAAGGAAGAGATGGAGAAGGAGAGCAGAGAAAGAG CATCGCTGGCCAGTCGCTATGACTCTCCCCTCCACTCAGCTTCCCATGCTCCATCATCTAAAACTTCATC TCTCCCTGGTTATGGAAAGAATGGCCTTCACCGGCCAGTTTCCACAGACTTCGCTCAGTACAACAGCTAC GGAGACATCAGTGGCGGAGTTCGAGACTACCAGACCCTCCCAGATGGCCACATGCCTGCAGTGAGAATGG ACAGAGGAGTATCCATGCCCAACATGTTGGAACCAAAGATATTTCCATATGAGATGCTCATGGTGACCAA CAGAGGGCGCAACAAAATCCTGAGAGATGTGGATAGAACCCGGCTAGAGCGCCACTTAGCCCCAGAAGTA TTTTGGGAGATCTTTGGGATGTCCATACAGGAATTTGACAAGTTACCTCTTTGGAGACGCAACGACATGA AGAAAAAAGCTAAACTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231009 representing NM_001290813 Red=Cloning site Green=Tags(s) MPSLLGLKCLGKLCSSEIGKVPSPERASLRNSHRRLLIEDLSVPETPDPAHRRRGTVIHLVYLYSAGCGP PELRFSSYDPSVAHPQDPHHSSEKPVIHCHKCGEPCKGEVLRVQTKHFHIKCFTCKVCGCDLAQGGFFIK NGDYLCTLDYQRMYGTRCHGCGEFVEGEVVTALGKTYHPNCFACTICKRPFPPGDRVTFNGRDCLCQLCA QPMSSSPKEASCSSNCAGCGRDIKNGQALLALDKQWHLGCFKCKSCGKVLTGEYISKDGSPYCEKDYQGL FGVKCEACHQFITGKVLEAGDKHYHPSCARCSRCNQMFTEGEEMYLQGSTVWHPDCKQSTKTEEKLRPTR TSSESIYSRPGSSIPGSPGHTIYAKVDNEILDYKDLAAIPKVKAIYDIERPDLITYEPFYTSGYEDKQER QSLGESPRTLSPTPSAEGYQDVRDRMIHRSTSQGSINSPVYSRHSYTPTTSRSPQHFHRPGNEPSSGRNS PLPYRPDSRPLTPTYAQAPKHFHVPDQGINIYRKPPIYKQHGTDPRRRSSGREEDEEELLRRRQLQEEQL MKLNSGLGQLILKEEMEKESRERASLASRYDSPLHSASHAPSSKTSSLPGYGKNGLHRPVSTDFAQYNSY GDISGGVRDYQTLPDGHMPAVRMDRGVSMPNMLEPKIFPYEMLMVTNRGRNKILRDVDRTRLERHLAPEV FWEIFGMSIQEFDKLPLWRRNDMKKKAKLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290813
ORF Size	2190 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290813.2
RefSeq Size	6245 bp
RefSeq ORF	2193 bp
Locus ID	226251
UniProt ID	Q8K4G5
MW	82.8 kDa
Gene Summary	May act as scaffold protein (By similarity). May play a role in the development of the retina. Has been suggested to play a role in axon guidance.[UniProtKB/Swiss-Prot Function]

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