Fign (NM_001267846) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	fi; fidget
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231051 representing NM_001267846 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGTGGACGCCGGAGCATGCCCAGTGGCCAGAACAGCACTTTGACATCACCTCGACCACTCGGTCTC CCGCCCACAAAGTTGAAGCCTATAGAGGTCATTTACAGCGCACCTACCAGTATGCCTGGGCGAATGATGA CATATCTGCTCTGACTGCGTCCAACCTTCTGAAAAAATATGCAGAGAAGTATTCTGGCATTTTGGAAGGT CCCGTAGACCGACCTGTACTCAGCAATTATTCAGACACACCATCAGGACTCGTGAATGGTCGGAAAAATG ATAGCGAACCCTGGCAGCCTTCCTTGAATTCAGAAGCCGTTTATCCTATGAACTGTGTCCCGGATGTCAT CACTGCCAGCAAAGCTGGTGTCAGCTCAGCCCTCCCTCCAGTAGACGTCTCGGCAAGTATAGGGAGCTCC CCTGGGGTGGCCAGCAACCTGACAGAGCCAAGTTACTCAAGTAGTACCTGTGGAAGCCACACAGTACCTA GTCTTCATGCGGGGCTCCCATCTCAGGAATATGCTCCGGGATACAACGGCTCATATTTGCATTCTACCTA CAGCAGCCAGGCCACACCTGCACTTCCTTCGCCTCATCCGTCCCCGTTGCATAGTTCTGGGCTTTTGCAG CCACCACCCCCTCCTCCTCCCCCACCAGCCCTGGTCCCAGGCTACAACGGGACTTCCAATCTCTCCAGTT ACAGTTATCCCTCTGCTAGCTATCCTCCTCAGACTGCCGTGGGGTCTGGGTACAGCCCTGGCGGAGCACC CCCTCCTCCTTCAGCATACCTGCCTTCAGGAATTCCTGCTCCCACCCCTCTGCCCCCCACTACCGTTCCT GGCTACACCTACCAAGGTCATGGTTTGACACCCATTGCACCCTCTGCTCTGACCAACAATTCAGCAAGTT CTCTCAAAAGGAAAGCTTTCTATATGGCAGGGCAAGGAGACATGGACTCCAGTTACGGAAATTACAGCTA TGGCCAACAGAGATCGACACAGAGTCCTATGTACCGAATGCCCGACAACAGCATTTCAAACTCGAATCGG GGGAATGGCTTTGACAGAAATGCTGAAACATCATCCTTAGCATTTAAGCCAACAAAGCAGCTGATGCCCT CTGAGCAGCAAAGGAAATTCAGCAGCCAGTCCAGTAGGGCTCTGACCCCTCCTTCCTATAGTACTGCTAA AAATTCATTGGGATCAAGGTCCAGTGAATCCTTTGGGAAGTATACGTCGCCAGTCATGAGTGAGCACGGA GACGACCATCGGCAGCTCCTCGCTCATCCAATACAAGGTCCCGGACTCCGTGCAGCTACCTCATCCAACC ACTCTGTGGACGAGCAACTGAAGAATACTGACACACACCTCATTGACCTGGTCACCAATGAGATCATCAC CCAAGGACCTCCTGTGGACTGGAGCGACATAGCTGGTCTTGACCTGGTGAAGGCGGTTATTAAGGAGGAG GTTTTGTGGCCAGTGCTGAGGTCAGATGCATTCAGTGGGCTGACCGCCTTACCTAGAAGCATCCTTCTCT TCGGACCTCGGGGTACAGGCAAAACACTTTTGGGCAGATGCATAGCTAGTCAGCTAGGGGCCACGTTTTT CAAAATTGCCGGTTCCGGACTCGTTGCAAAGTGGATAGGAGAAGCGGAGAAAATCATCCACGCCTCTTTT CTTGTGGCCAGGTGTCGCCAGCCCTCGGTGATTTTTGTTAGTGACATTGACATGCTTCTCTCCTCTCAAG TGAGCGAGGAACACAGTCCAGTCAGTCGGATGAGAACCGAATTTCTGATGCAGCTGGACACTGTACTGAC TTCGGCTGAGGACCAAATCGTAGTCATTTGTGCCACCAGTAAACCAGAAGAAATAGATGAATCTCTTCGG AGGTACTTCATGAAAAGACTTTTAATCCCACTTCCTGACAGCACAGCGAGGCACCAGATAATAGTACAGC TGCTCACACAGCACAATTACTGTCTCAATGACAAGGAGTTTGCACTGCTTGTCCAGCGCACAGAAGGCTT TTCTGGACTAGACGTGGCTCATTTGTGTCAGGAAGCAGCAGTGGGACCTCTCCATGCTATGCCAGCTACA GACCTTTCAGCCATTATGCCCAGCCAGTTAAGGCCCGTTACATATCAAGACTTTGAAAATGCTTTCTGCA AGATTCAGCCTAGCATATCTCAAAAAGAGCTTGATATGTATGTTGAATGGAACAAAATGTTTGGTTGCAG TCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231051 representing NM_001267846 Red=Cloning site Green=Tags(s) MQWTPEHAQWPEQHFDITSTTRSPAHKVEAYRGHLQRTYQYAWANDDISALTASNLLKKYAEKYSGILEG PVDRPVLSNYSDTPSGLVNGRKNDSEPWQPSLNSEAVYPMNCVPDVITASKAGVSSALPPVDVSASIGSS PGVASNLTEPSYSSSTCGSHTVPSLHAGLPSQEYAPGYNGSYLHSTYSSQATPALPSPHPSPLHSSGLLQ PPPPPPPPPALVPGYNGTSNLSSYSYPSASYPPQTAVGSGYSPGGAPPPPSAYLPSGIPAPTPLPPTTVP GYTYQGHGLTPIAPSALTNNSASSLKRKAFYMAGQGDMDSSYGNYSYGQQRSTQSPMYRMPDNSISNSNR GNGFDRNAETSSLAFKPTKQLMPSEQQRKFSSQSSRALTPPSYSTAKNSLGSRSSESFGKYTSPVMSEHG DDHRQLLAHPIQGPGLRAATSSNHSVDEQLKNTDTHLIDLVTNEIITQGPPVDWSDIAGLDLVKAVIKEE VLWPVLRSDAFSGLTALPRSILLFGPRGTGKTLLGRCIASQLGATFFKIAGSGLVAKWIGEAEKIIHASF LVARCRQPSVIFVSDIDMLLSSQVSEEHSPVSRMRTEFLMQLDTVLTSAEDQIVVICATSKPEEIDESLR RYFMKRLLIPLPDSTARHQIIVQLLTQHNYCLNDKEFALLVQRTEGFSGLDVAHLCQEAAVGPLHAMPAT DLSAIMPSQLRPVTYQDFENAFCKIQPSISQKELDMYVEWNKMFGCSQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001267846
ORF Size	2244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267846.1, NP_001254775.1
RefSeq Size	9817 bp
RefSeq ORF	2247 bp
Locus ID	60344
UniProt ID	Q9ERZ6
MW	81.4 kDa
Gene Summary	ATP-dependent microtubule severing protein. Severs microtubules along their length and depolymerizes their ends, primarily the minus-end, suppressing microtubule growth from and attachment to centrosomes. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome (By similarity).[UniProtKB/Swiss-Prot Function]

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