Cars (NM_001252593) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CA3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231053 representing NM_001252593 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGGTTCCTCCGCGGAGCAGGGTAAAGGCCGGCGAGTGCAGCCTCAGTGGTCCCCCCCAGCAGGGA CTGAGCCCTGCAGGCTCCGCCTCTATAATAGCCTCACCCGGAACAAGGATGTATTTATACCTCAAGATGG GAAGAAGGTGACGTGGTACTGTTGCGGGCCGACTGTCTATGATGCGTCTCACATGGGACATGCCAGGTCC TACATCTCCTTCGATATCCTGAGGAGGGTGCTGAGGGATTACTTCCAGTATGATGTCTTTTACTGCATGA ACATCACAGACATTGATGATAAGATCATCAGGCGGGCACGGCAGAACTACCTGTTTGAGCAGTATCGGGA GCAGAAACCCCCAGCCACCCAGCTCCTGAAGGATGTCCGTGATGCCATGAAGCCATTTTCAGTCAAGTTA AGTGAGACAACAGATCCCGACAAGAGGCAGATGCTGGAGCGGATCCAGAACTCTGTGAAACTTGCCACAG AACCACTGGAACAGGCTGTGCGATCCAGCCTCTCTGGAGAGGAAGTAGACAGCAAAGTACAGGTGTTGCT GGAGGAAGCAAAGGACTTGCTCTCTGACTGGCTGGATTCCACAGGTGGCAGTGAGGTGACTGACAACTCG ATTTTCTCCAAACTGCCCAAGTTCTGGGAAGAAGAGTTCCACAAAGACATGGAAGCCCTGAATGTTCTCC CTCCTGATGTCCTAACCCGTGTCAGTGAATATGTGCCAGAAATTGTGAACTTCGTCCAGAAGATTGTGGA CAATGGTTATGGTTATGCTTCAAATGGATCTGTTTACTTCGACACAGCCAAGTTTGCTGCTAGTGAAAAG CACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGGGATCAGAAGGCACTTCAGGAAGGGGAAGGTGATC TGAGCATCTCTGCTGACCGCCTTAGTGAGAAACGCTCTCCCAATGACTTTGCCTTGTGGAAGGCCTCCAA GCCAGGCGAGCCATCCTGGCCTTGCCCCTGGGGAAAGGGTCGTCCCGGATGGCACATAGAGTGTTCTGCT ATGGCAGGCACGCTCCTGGGAGCCTCAATGGACATTCATGGTGGAGGGTTTGACCTCCGCTTCCCCCACC ATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAATGACTGCTGGGTCAGGTACTTCTTGCACAC GGGCCACTTGACGATAGCAGGCTGCAAGATGTCCAAGTCACTGAAAAACTTTATCACCATTAAAGATGCC TTGAAGAAGCACTCAGCACGGCAGCTGCGACTGGCATTCCTCATGCACTCATGGAAAGACACACTGGACT ATTCCAGCAACACTATGGAGTCTGCTCTTCAGTATGAAAAATTCATGAACGAGTTTTTCTTAAATGTGAA AGACATCCTCCGAGCCCCTGTAGACATCACTGGCCAGTTTGAAAAGTGGGAAGCTGAAGAAGTGGAGCTA AATAAGAACTTCTATGGCAAGAAGACCGCAGTTCACGAAGCCTTGTGTGATAACATTGACACCCGGACTG TCATGGAAGAGATGCGGGCTTTGGTCAGTCAGTGCAACCTCTACATGGCAGCCAGGAAGGCTGAACGGAG GAGGCCCAACCGGGCTCTGCTGGAGAACATTGCCATGTACCTCACCCACATGCTGAAGATCTTTGGGGCC ATAGAGGAGGAGAGCCCCCTGGGGTTCCCAGTTGGTGGGCCTGGAACCAACCTGAACCTCGAGTCAACAG TCATGCCCTACCTTCAGGTGTTATCAGAATTCAGAGAGGGAGTACGAAAGATTGCCCGAGAGAAAAAAGT TCTTGAGGTTCTACAGCTCAGTGATGCCCTCCGGGATGACATCCTGCCTGAGCTTGGGGTCCGGTTTGAA GACCATGAAGGGCTGCCAACAGTGGTGAAGTTGGTGGACAGAGACACCTTACTGAAAGAGAAGGAAGGAA AGAAAAGGGCTGAAGAAGAGAAGAGGAGGAAGAAAGAGGAGGCGGCCAGAAAGAAACAGGAGCAAGAAGC AGCAAAGCTGGCCAAGATGAAGATACCACCCAGTGAGATGTTCCTGTCAGAAGTCAACAAGTATTCTAAA TTTGATGAAAATGGTCTGCCTACCCACGACACCGAAGGCAAAGAGCTGAGCAAGGGCCAGGCCAAGAAGC TGAAGAAGCTCTTTGAAGCCCAGGAGAAACTGTACAAGGAGTATCTGCAAATGTTACAGAATGGCAGCCT CCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231053 representing NM_001252593 Red=Cloning site Green=Tags(s) MAGSSAEQGKGRRVQPQWSPPAGTEPCRLRLYNSLTRNKDVFIPQDGKKVTWYCCGPTVYDASHMGHARS YISFDILRRVLRDYFQYDVFYCMNITDIDDKIIRRARQNYLFEQYREQKPPATQLLKDVRDAMKPFSVKL SETTDPDKRQMLERIQNSVKLATEPLEQAVRSSLSGEEVDSKVQVLLEEAKDLLSDWLDSTGGSEVTDNS IFSKLPKFWEEEFHKDMEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNGYGYASNGSVYFDTAKFAASEK HSYGKLVPEAVGDQKALQEGEGDLSISADRLSEKRSPNDFALWKASKPGEPSWPCPWGKGRPGWHIECSA MAGTLLGASMDIHGGGFDLRFPHHDNELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKSLKNFITIKDA LKKHSARQLRLAFLMHSWKDTLDYSSNTMESALQYEKFMNEFFLNVKDILRAPVDITGQFEKWEAEEVEL NKNFYGKKTAVHEALCDNIDTRTVMEEMRALVSQCNLYMAARKAERRRPNRALLENIAMYLTHMLKIFGA IEEESPLGFPVGGPGTNLNLESTVMPYLQVLSEFREGVRKIAREKKVLEVLQLSDALRDDILPELGVRFE DHEGLPTVVKLVDRDTLLKEKEGKKRAEEEKRRKKEEAARKKQEQEAAKLAKMKIPPSEMFLSEVNKYSK FDENGLPTHDTEGKELSKGQAKKLKKLFEAQEKLYKEYLQMLQNGSLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252593
ORF Size	2244 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252593.1, NP_001239522.1
RefSeq Size	2987 bp
RefSeq ORF	2247 bp
Locus ID	27267
UniProt ID	Q9ER72
MW	86 kDa
Gene Summary	Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).[UniProtKB/Swiss-Prot Function]

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