Apbb2 (NM_001201413) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310007D03Rik; FE65L1; Rirl1; TR2L; Zfra
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231087 representing NM_001201413 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGAAGTACTTCCAGCTGACTCAGGTGTTGGCACCTTGGCAGTGTTTATGGCCAGCAGCGGATCCA CAGACATTGCAAACCGGAACAGCCCAGCCACACCACCAAATACCCTCAATCTCCGTTCCTCCCACAATGA ACTATTAAATGCAGAGATCAAACACTCAGATGCCAAGAACAGCACGCCCCCCAAATGCAGGAAAAAATAT GCACTGACTAATATTCAGGCGGCCATGGGCCTCTCGGATCCAGCTGTACAGCCCCTGCTGGGAAACGGCT CTGCCAACATCAAGCTGGTTAAAAATGGGGAGAACCAGCTCCGCAAGGCTGCAGAACAGGGGCAGCAGGA CCCCAACAAGAACCTCAGCCCTGCAGCCGTCATCAACCTAACCTCCGAGAAGCTGGAGGTTAAAGATCCC CACCCACAGGAGTCTTCAGGCTGTGAGATTTTGCCCTCCCAGCCCAGGAGAACTAAGAGCTTCCTCAATT ACTATGCAGACCTGGAAACCTCGGCTAGAGAGCTAGGGCAGAACCTGGGCCCCTGCCAAGGGGTTGGAGA GGAGAAAGCCCAGCCTGGCCCAGGCCAGGCCCCAGTAGTCATTGGGAATGGTGACTTGCTGCCACAGAAA CCAAACAAACCCCAATCCAGCCCAGAGGATGGCCAAGTAGCCACAGTATCGTCCAGCCCAGAGACCAAGA AAGACCACCCTAAAACAGGAGCCAAAACCGACTGTGCCCTCCATCGGATCCAGAACCTGGCACCAAGTGA CGAGGAATCCAGCTGGACGACGCTGTCCCAAGACAGTGCCTCCCCCAGCTCCCCAGATGAAACAGATATC TGGAGTGATCACTCATTTCAGACAGATCCCGATTTGCCGCCGGGCTGGAAGAGAGTCAATGACATTGCCG GGACCTATTACTGGCACATCCCAACAGGGACGACTCAATGGGAACGGCCTGTCTCCATCCCAGCAGATCT CCACGGCTCTAGGAAAGGGTCACTTAGTTCTGTAACGCCATCGCCCACCCCAGAGAATGAGAAACAGCCA TGGAGTGATTTTGCTGTTCTGAATGGGGGAAAGATTAATAGTGACATTTGGAAGGATTTGCACGCAGCCA CAGTTAACCCAGACCCCAGTTTAAAAGAGTTTGAAGGAGCAACGCTACGCTATGCATCCTTGAAACTCAG AAACGCCCCTCATGGTGATGACGATGATTCTTGTAGTATCAACAGTGACCCAGAAGCCAAGTGTTTTGCT GTGCGTTCTCTGGGCTGGGTAGAGATGGCCGAGGAGGACCTTGCCCCCGGGAAGAGCAGTGTCGCTGTCA ACAACTGCATCCGACAGCTTTCCTACTGCAAAAACGACATCCGGGACACAGTCGGCATCTGGGGAGAGGG CAAAGACATGTACCTGAGCCTGGAAAACGACATGCTCAGCCTGGTGGACCCCATGGACCGCTCCGTGTTA CACTCCCAGCCCATCGTGAACATCCGAGTGTGGGGCGTGGGCCGAGACAACGGCCGGGACTTTGCTTACG TGGCGAGAGACAAGGACACAAGGATTCTGAAATGCCATGTGTTTCGATGTGACACACCAGCAAAAGCCAT TGCCACAAGTCTCCACGAAATCTGCTCCAAGATTATGGCTGAACGGAAGAACGCCAAAGCACTGGCCTGC AGCTCCTTACAGGAGAGGACCAATATGAGTCTCGATGTCCCTTTGCAAGTAGATTTTCCAACACCAAAGA CGGAGCTGGTGCAGAAGTTCCGCGTGCAGTACCTGGGCATGTTACCTGTAGACAGACCTGTCGGCATGGA CACCCTGAACAGTGCCATAGAAAATCTCATGACGTCATCCAGCAAGGAGGACTGGCCTTCGGTGAACATG AACGTGGCCGACGCCACTGTGACTGTCATCAGTGAAAAGAATGAAGAGGAGGTCTTGGTGGAGTGTCGAG TGCGGTTCCTGTCCTTCATGGGTGTCGGGAAGGATGTCCACACATTCGCCTTCATCATGGACACTGGGAA CCAGCGCTTTGAGTGCCATGTGTTCTGGTGTGAGCCTAACGCAGCCAATGTGTCAGAAGCTGTCCAGGCT GCCTGCATGTTGCGGTATCAGAAGTGCTTGGTTGCCAGGCCACCTTCGCAGAAAGTCCGGCCCCCGCCCC CGCCAGCAGATTCAGTGACCCGAAGAGTCACGACCAATGTGAAACGAGGGGTCTTATCCCTCATTGACAC TTTGAAACAGAAGCGGCCGGTCACAGAGACTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231087 representing NM_001201413 Red=Cloning site Green=Tags(s) MSEVLPADSGVGTLAVFMASSGSTDIANRNSPATPPNTLNLRSSHNELLNAEIKHSDAKNSTPPKCRKKY ALTNIQAAMGLSDPAVQPLLGNGSANIKLVKNGENQLRKAAEQGQQDPNKNLSPAAVINLTSEKLEVKDP HPQESSGCEILPSQPRRTKSFLNYYADLETSARELGQNLGPCQGVGEEKAQPGPGQAPVVIGNGDLLPQK PNKPQSSPEDGQVATVSSSPETKKDHPKTGAKTDCALHRIQNLAPSDEESSWTTLSQDSASPSSPDETDI WSDHSFQTDPDLPPGWKRVNDIAGTYYWHIPTGTTQWERPVSIPADLHGSRKGSLSSVTPSPTPENEKQP WSDFAVLNGGKINSDIWKDLHAATVNPDPSLKEFEGATLRYASLKLRNAPHGDDDDSCSINSDPEAKCFA VRSLGWVEMAEEDLAPGKSSVAVNNCIRQLSYCKNDIRDTVGIWGEGKDMYLSLENDMLSLVDPMDRSVL HSQPIVNIRVWGVGRDNGRDFAYVARDKDTRILKCHVFRCDTPAKAIATSLHEICSKIMAERKNAKALAC SSLQERTNMSLDVPLQVDFPTPKTELVQKFRVQYLGMLPVDRPVGMDTLNSAIENLMTSSSKEDWPSVNM NVADATVTVISEKNEEEVLVECRVRFLSFMGVGKDVHTFAFIMDTGNQRFECHVFWCEPNAANVSEAVQA ACMLRYQKCLVARPPSQKVRPPPPPADSVTRRVTTNVKRGVLSLIDTLKQKRPVTETP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001201413
ORF Size	2274 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001201413.1, NP_001188342.1
RefSeq Size	6665 bp
RefSeq ORF	2277 bp
Locus ID	11787
MW	83.4 kDa
Gene Summary	May modulate the internalization of amyloid-beta precursor protein.[UniProtKB/Swiss-Prot Function]

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