Pcsk7 (NM_001281934) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA959856; PC7; SPC7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231113 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAAGGGAGGCAGAAAGTCCCACACTTGGATGCCCACCTGGGCCTGCCCATCTGCCTCTGGCTGG AATTAGCCATCTTCTTTCTGGTTCCCCAGGTCATGGGCCTATCAGAGGCAGGTGGGCTTGACATCTTGGG CACAGGGGGGCTGAGCTGGGCCGTACATCTGGACAGCCTAGAAGGTGAGAGGAAGGAAGAGAGTCTGACG CAACAGGCCGATGCTGTGGCCCAGGCAGCAGGGCTTGTGAATGCTGGGCGCATTGGAGAACTCCAGGGGC ACTACCTCTTTGTCCAGCCTACTGGGCATAGGCAAGCCATGGAGGTGGAGGCCATGCGGCAACAGGCAGA GGCTGTGTTAGCCAGGCATGAAGCTGTGCGCTGGCACTCGGAGCAAACGCTGCTGAAGAGGGCCAAGCGC AGTATCCACTTCAATGATCCCAAGTACCCTCAACAGTGGCACCTGAATAATCGGCGGAGCCCAGGAAGAG ACATCAATGTGACAGGTGTGTGGGAGCGAAATGTAACTGGGCGTGGGGTGACAGTGGTGGTGGTGGATGA CGGAGTGGAACACACCGTCCAGGACATCGCACCCAACTACAGTCCAGAGGGAAGTTATGACCTCAACTCT AATGACCCAGATCCCATGCCCCACCCTGATGAGGAGAATGGTAACCACCATGGGACCCGGTGTGCAGGAG AAATTGCAGCTGTGCCCAACAACAGCTTCTGTGCAGTGGGTGTGGCCTACGGGAGCCGAATAGCAGGTAT CCGGGTGCTGGATGGACCACTTACTGACAGTATGGAGGCTGTGGCGTTCAACAAGCACTATCAGATCAAT GACATCTACAGCTGCAGCTGGGGCCCAGATGATGATGGGAAGACAGTGGATGGTCCTCATCAGCTCGGAA AGGCTGCCTTACAACATGGAGTGATGGCTGGTCGCCAAGGCTTTGGAAGTATCTTTGTGGTTGCCAGTGG TAATGGGGGCCAGCACAATGACAACTGCAACTATGATGGCTATGCCAACTCCATCTACACTGTCACCATA GGAGCTGTGGATGAGGAGGGACGGATGCCTTTTTATGCAGAGGAGTGTGCCTCCATGCTGGCAGTCACCT TCAGTGGTGGAGACAAGATGCTGCGGAGCATTGTGACCACTGACTGGGACCTTCAGAAGGGCACTGGCTG CACTGAAGGCCACACAGGAACCTCAGCTGCAGCTCCTCTGGCAGCTGGAATGATAGCCCTCATGCTGCAG GTGCGGCCCTGCCTCACATGGCGGGATGTACAGCACATAATTGTCTTCACAGCCATCCAGTATGAAGATC ATCATGCAGACTGGCTCACCAATGAGGCTGGCTTCAGCCACAGCCATCAGCATGGTTTCGGCCTGCTCAA CGCTTGGAGACTTGTCAATGCAGCCAAGATCTGGACATCTGTCCCTTACTTAGCCTCCTATGTCAGCCCC ATGCTGAAAGAAAATAAGGCTGTTCCGAGGTCCCCCCACTCTCTGGAGGTGCTATGGAATGTCAGCAGGA CGGACCTGGAGATGTCGGGGCTGAAGACCTTGGAACATGTGGCAGTGACAGTCTCCATCACTCACCCACG ACGTGGCAGCTTAGAACTGAAACTGTTTTGTCCCAGTGGCATGATGTCTTTGATCGGCGCGCCCCGCAGC ATGGACTCGGATCCTAATGGCTTCAATGCTTGGACCTTTTCCACTGTGCGGTGCTGGGGGGAAAGAGCAA GAGGCGTCTACAGACTGGTTATCAGGGATGTAGGAGATGAGCCGCTCCAGATGGGCATCCTCCAGCAGTG GCAGCTGACCCTGTATGGCTCCATGTGGAGTCCAGTAGACATCAAGGACAGACAAAGTCTCTTAGAAAGT GCTATGAGTGGAAAATACCTGCATGATGGCTTCACCCTGCCTTGCCCACCTGGGCTGAAAATCCCTGAGG AGGATGGCTACACCATTACCCCTAACACACTCAAGACCCTTGTGCTGGTAGGCTGCTTCAGTGTCTTCTG GACTATTTACTACATGCTAGAAGTGTGCCTGAGCCAGAGGAACAAGGCTTCCACCCATGGCTGCAGGAAG GGATGCTGCCCCTGGGCCCCACGAAGGCAAAACTCCAAGGATGCAGGGACAGCACTAGAATCAATGCCAC TGTGCAGCAGCAAGGACCTGGATGGAGTGGATTCAGAGCATGGGGACTGCACAACTGCATCTAGCTTCCT GGCCCCAGAGCTGGATTGTCCTCCACACCAACCCCCAGACCTGCTGCAGGGGAAGAGCGGGCAGATTTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231113 protein sequence Red=Cloning site Green=Tags(s) MPKGRQKVPHLDAHLGLPICLWLELAIFFLVPQVMGLSEAGGLDILGTGGLSWAVHLDSLEGERKEESLT QQADAVAQAAGLVNAGRIGELQGHYLFVQPTGHRQAMEVEAMRQQAEAVLARHEAVRWHSEQTLLKRAKR SIHFNDPKYPQQWHLNNRRSPGRDINVTGVWERNVTGRGVTVVVVDDGVEHTVQDIAPNYSPEGSYDLNS NDPDPMPHPDEENGNHHGTRCAGEIAAVPNNSFCAVGVAYGSRIAGIRVLDGPLTDSMEAVAFNKHYQIN DIYSCSWGPDDDGKTVDGPHQLGKAALQHGVMAGRQGFGSIFVVASGNGGQHNDNCNYDGYANSIYTVTI GAVDEEGRMPFYAEECASMLAVTFSGGDKMLRSIVTTDWDLQKGTGCTEGHTGTSAAAPLAAGMIALMLQ VRPCLTWRDVQHIIVFTAIQYEDHHADWLTNEAGFSHSHQHGFGLLNAWRLVNAAKIWTSVPYLASYVSP MLKENKAVPRSPHSLEVLWNVSRTDLEMSGLKTLEHVAVTVSITHPRRGSLELKLFCPSGMMSLIGAPRS MDSDPNGFNAWTFSTVRCWGERARGVYRLVIRDVGDEPLQMGILQQWQLTLYGSMWSPVDIKDRQSLLES AMSGKYLHDGFTLPCPPGLKIPEEDGYTITPNTLKTLVLVGCFSVFWTIYYMLEVCLSQRNKASTHGCRK GCCPWAPRRQNSKDAGTALESMPLCSSKDLDGVDSEHGDCTTASSFLAPELDCPPHQPPDLLQGKSGQIC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001281934
ORF Size	2313 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281934.1, NP_001268863.1
RefSeq Size	4098 bp
RefSeq ORF	2313 bp
Locus ID	18554
UniProt ID	Q61139
MW	84.4 kDa
Gene Summary	Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive secretory pathway.[UniProtKB/Swiss-Prot Function]

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