Usp1 (NM_001301414) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231140 representing NM_001301414 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGGCGTCATACCTAGTGAAAGTAATGGGCTTTCAAGAGGCAGTCCATCCAAGAAAAACAGACTGT CTCTGAAGTTTTTTCAGAAAAAGGAAACTAAAAGAGCCTTAGACTTTACTGATTCTCAAGAAAATGAAGA AAAAACTTCTGAATATAGAGGATCTGAGATTGACCAAGTTGTTCCTGCTGCACAGTCCTCCCCAGTAAGC TGCGAGAAGAGAGAGAACTTGTTACCATTTGTGGGATTGAATAACCTTGGCAACACTTGTTATCTGAATA GTATTCTTCAGGTGTTATACTTTTGTCCTGGCTTTAAGACTGGAGTGAAGCACTTATTTAATATTATTTC AAGGAAGAAAGAAGCTCTGAAGGATGACTCTAATCAGAAGGATAAGGGAAGCTGCAAAGAAGAGTCTTTG GCAAGTTATGAGCTTATATGTAGTTTACAGTCCTTAATAATTTCAGTTGAACAGCTTCAGGCTAGTTTTC TCTTAAATCCAGAGAAATATACAGATGAACTTGCTACACAGCCAAGGCGACTGCTGAACACACTCAGGGA ACTCAACCCTATGTATGAAGGATTTCTACAGCATGATGCACAGGAAGTGCTACAGTGTATTCTGGGAAAC ATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAATAAAAAACCTGGCTGAATTATCCGGCAAGGTTG AAGAACAATCTCTTCAGAAAGAGGAAACAGGTGGAATTACCAGCACAGAGATTGACAGTATGAGGAATAC GGAGGACGTCAAAGAGCAGCTCCCCAAAGGGAACTGGAAAAGAAAAAGTGACAGTGAGTCGAGTAACGTG AAGAAAAAGGTTAAACTGTCCAGGGAATCCCAGCCATTGGAAGAAAACCAGAGACAAACCAGATCCAAGA GAAAAGCTATCGGAGACACACTAGAGGCTGCTCCTAAGATCATCCCCAAGTGTGTTTCTGAAAGTGAGAG TGCAAAGCCCTCACAGAAGAAATCAAAAGTTAAAATAAATTGGTTAAAGCCATCCACTAAGCAACCCAGC ATTCTTTCCAAGTTCTGTAGTCTTGGAAAAATAACAACTAACCAGCGCTCCAAAGGACAACCTAAGGAAA AGGAGGGTGATGTTGAAGAGGACTTGGAGAAGTACGGAAGTGACCACACAGCTAATGGTGGACCCGAATC TCCAGGAAGTAGCGTCACACCTGTGGACAGCAGTGAAGCCAAGTCCGGAAACAAAGGTGCTGAACAGATT GGCTTTGAGCTAGTAGAGAAACTGTTTCAAGGTCAGCTCGTGTTAAGGACTCGGTGTTTGGAATGTGAAA GCTTAACAGAAAGAAGAGAAGATTTCCAGGATATTAGTGTTCCAGTGCAAGAAGATGAGCTTTCCAAGGT CGAGGAGAGCTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCTTGAGATGGGCAATTTCACAG TTTGCTTCAGTGGAGAGAATTGTAGGAGAGGATAAATACTTCTGTGAAAATTGCCATCATTATACGGAAG CAGAGCGAAGTCTCCTGTTTGATAAAATGCCTGAAGTTATCACTATTCATCTGAAGTGCTTTGCTGCTAG TGGCTTGGAGTTTGACTGTTACGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAA CTGTCACTAGAAGAATGGAGCACAAAGCCGACGAATGACAGCTATGGATTATTTGCCGTTGTGATGCACA GTGGCATTACTATTAGTAGTGGGCACTATACCGCATCTGTTAAAGTCACTGACCTTAACAGTCTAGAGCT CGATGAGGGGAATTTTGTGGTTGACCAGATGTGTGAACTAGGTAAGCCAGAGCCACTGACCGAGGAGCAA GCAAGGGGGACGGCTGGGAACTACGACGATGAAGTATCAATTAGAGTTGGTGGAAATGCCCAGCCAAGTA AAGTTTTGAACAAAAAAAATGTAGAAGGTATTGGACTTCTCGGTGGACAAAAGAGCAAAGCAGATTATGA GCTGTACAACAAAGCATCTAATCCTGATAAGGTGGTTGGGACACCATTCACCGACAATAGAAATTCTGAA ACTAATGATACTACTAATGGGACCCACGAATCTGACAGGAACAAGGAATCCAGTGACCAAACAGGCGTTA ACATGAACGGACTTGAGAACAAAATTTCCTATGTAGTGCAAAGCTTAAAGGAGTACGAGGGGAAGTGGTT GCTCTTTGACGATTCTGAGGTAAAAGTGACAGAAGAGAAAGACTTTCTGAATTCTCTCTCCCCTTCTACA TCCCCTACGTCTACTCCCTACTTGCTATTCTATAAAAAATTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231140 representing NM_001301414 Red=Cloning site Green=Tags(s) MPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKTSEYRGSEIDQVVPAAQSSPVS CEKRENLLPFVGLNNLGNTCYLNSILQVLYFCPGFKTGVKHLFNIISRKKEALKDDSNQKDKGSCKEESL ASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGFLQHDAQEVLQCILGN IQETCQLLKKEEIKNLAELSGKVEEQSLQKEETGGITSTEIDSMRNTEDVKEQLPKGNWKRKSDSESSNV KKKVKLSRESQPLEENQRQTRSKRKAIGDTLEAAPKIIPKCVSESESAKPSQKKSKVKINWLKPSTKQPS ILSKFCSLGKITTNQRSKGQPKEKEGDVEEDLEKYGSDHTANGGPESPGSSVTPVDSSEAKSGNKGAEQI GFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQ FASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLK LSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDEGNFVVDQMCELGKPEPLTEEQ ARGTAGNYDDEVSIRVGGNAQPSKVLNKKNVEGIGLLGGQKSKADYELYNKASNPDKVVGTPFTDNRNSE TNDTTNGTHESDRNKESSDQTGVNMNGLENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPST SPTSTPYLLFYKKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001301414
ORF Size	2352 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301414.1, NP_001288343.1
RefSeq Size	3608 bp
RefSeq ORF	2355 bp
Locus ID	230484
UniProt ID	Q8BJQ2
MW	87.5 kDa
Gene Summary	This gene encodes a member of the ubiquitin-specific peptidase family. The encoded protein acts as a catalytic subunit in a heterodimeric deubiquitinating enzyme complex that deubiquitinates Fanconi anemia, complementation group D2, and plays a role in homologous recombination-mediated DNA repair. Disruption of this gene is associated with a Fanconi anemia-like phenotype and genomic instability. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 3, 12, and 15. [provided by RefSeq, Aug 2014]

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