Arhgap6 (NM_001287530) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI504284; RhoGAPX-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231158 representing NM_001287530 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAAAATATTTGGTGACTTCACCTGGAACAGCATGTCTGGTCGTAGCGTGCGCCTGAGGTCAGTCC CCATCCAGAGCCTCTCAGAGCTGGAGCGGGCACGACTGCAGGAAGTGGCTTTTTATCAGTTGCAGCAGGA CTGTGACCTGGGCTGTCAGATCACCATCCCCAAAGATGGACAAAAGAGAAAGAAATCTTTGAGAAAGAAA CTGGATTCACTAGGGAAGGAAAAGAACAAAGACAAAGAATTCATCCCACAGGCATTTGGAATGCCCTTAT CCCAAGTCATTGCTAATGACCGGGCATATAAACTGAAGCAAGACTTGCAGAGGGAGGAGCAGAAGGATGC ATCATCGGATTTTGTGTCTTCCCTCCTCCCATTTGGGAATAAAAAACAAAACAAAGAACTCTCAAGCAGT AACTCATCTCTCAGCTCAACCTCAGAAACACCAAATGAGTCTACATCACCGAATACTCCAGAACCAGCTC CTCGGGCCAGGAGAAGGGGCGCCATGTCCGTGGATTCCATCACTGATCTGGATGACAACCAGTCTCGACT CCTAGAAGCTTTACAACTCTCCTTGCCTGCTGAGGCTCAGAGTAAAAAAGAAAAGGCCAGAGATAAGAAG CTGAGTCTGAATCCTATTTACAGGCAGGTCCCCAGGCTGGTGGACAGCTGCTGTCAACATCTGGAAAAAC ATGGCCTCCAGACAGTGGGGATATTCCGAGTTGGAAGCTCAAAGAAGAGAGTAAGACAATTGCGTGAAGA ATTTGACCGTGGGGTTGATGTCTGTCTGGAAGAGGAGCATAGTGTTCACGATGTGGCAGCCTTGTTAAAG GAGTTCCTTAGAGACATGCCTGACCCCCTTCTCACAAGGGAGCTATACACTGCATTTATCAACACTCTCC TGTTGGAGCCTGAGGAACAACTGGGCACCTTGCAACTCCTCATTTACCTTCTACCTCCCTGCAACTGCGA CACCCTCCACCGCCTCCTACAGTTCCTCTCCATTGTGGCCAGGCATGCTGATGATAATGTCAGCAAAGAT GGACAAGAGGTTACTGGGAACAAAATGACATCTCTGAACTTAGCCACTATATTTGGACCCAACCTGCTCC ACAAGCAGAAGTCATCAGACAAAGAATATTCTGTTCAGAGCTCAGCCAGAGCTGAGGAGAGCACAGCCAT CATAGCTGTGGTACAGAAGATGATTGAAAATTATGAAGCCTTGTTCATGGTTCCCCCAGATCTCCAGAAT GAAGTGCTGATCAGCCTTCTAGAGACAGATCCAGATGTTGTGGACTACTTGCTCAGAAGAAAGGCTTCCC AATCCTCGAGCCCTGACATACTTCAGACGGAAGTTTCCTTTTCCATGGGAGGGAGGCATTCATCTACAGA TTCCAACAAAGCCTCCAGTGGAGACATCTCCCCTTATGACAACAACTCCCCAGTATTGTCTGAGCGCTCC CTGCTGGCTATGCAAGAGGACAGGGCCCGGGGGGGCTCGGAGAAGCTTTATAAAGTGCCAGAGCAGTATA CACTGGTGGGCCACTTGTCATCGCCAAAGTCAAAGTCAAGAGAAAGTTCTCCTGGACCAAGGCTTGGAAA AGAAATGTCAGAGGAGCCTTTCAATATCTGGGGAACTTGGCATTCAACATTAAAAAGTGGATCCAAAGAC CCAGGAATGACAGGCTCTTATGGCGACATTTTTGAAAGCAGCTCCCTCCGACCGAGGCCTTGTTCTCTTT CTCAAGGGAACCTTTCCCTGAACTGGCCTCGGTGTCAAGGGAGCCCGACAGGGCTGGACAGTGGCACTCA GGTAATTCGGAGGACTCAGACGGCGGCCACCGTGGAGCAGTGCAGTGTCCACCTTCCGGTGTCACGTGTC TGCAGCACTCCCCACATCCAGGACGGCAGCAGGGGGACCAGGCGGCCTGCAGCCAGCTCTGATCCATTTT TGTCCCTAAACAGCACAGAAGATCTAGCTGAGGGCAAGGAGGATGTTGCCTGGCTGCAAAGCCAGGCCCG ACCTGTGTACCAGAGACCTCAGGAGAGTGGAAAAGATGACAGGCGCCCCCCTCCTCCTTACCCGGGGTCA GGGAAGCCTGCCACAACCTCTGCCCAGCTGCCACTAGAGCCTCCCCTGTGGAGGCTCCAGAGGCATGAAG AAGGTTCAGAAACAGCTGTGGAAGGAGGCCAGCAGGCCTCAGGGGAGCATCAGACCAGGCCAAAAAAACT GAGCAGCGCCTACTCCCTCTCAGCCAGCGAGCAGGACAAACAGAACTTAGGGGAAGCCAGCTGGCTCGAC TGGCAGCGAGAGCGGTGGCAGATCTGGGAGCTTCTATCAACTGATAACCCCGACGCCCTCCCGGAAACCC TAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231158 representing NM_001287530 Red=Cloning site Green=Tags(s) MYKIFGDFTWNSMSGRSVRLRSVPIQSLSELERARLQEVAFYQLQQDCDLGCQITIPKDGQKRKKSLRKK LDSLGKEKNKDKEFIPQAFGMPLSQVIANDRAYKLKQDLQREEQKDASSDFVSSLLPFGNKKQNKELSSS NSSLSSTSETPNESTSPNTPEPAPRARRRGAMSVDSITDLDDNQSRLLEALQLSLPAEAQSKKEKARDKK LSLNPIYRQVPRLVDSCCQHLEKHGLQTVGIFRVGSSKKRVRQLREEFDRGVDVCLEEEHSVHDVAALLK EFLRDMPDPLLTRELYTAFINTLLLEPEEQLGTLQLLIYLLPPCNCDTLHRLLQFLSIVARHADDNVSKD GQEVTGNKMTSLNLATIFGPNLLHKQKSSDKEYSVQSSARAEESTAIIAVVQKMIENYEALFMVPPDLQN EVLISLLETDPDVVDYLLRRKASQSSSPDILQTEVSFSMGGRHSSTDSNKASSGDISPYDNNSPVLSERS LLAMQEDRARGGSEKLYKVPEQYTLVGHLSSPKSKSRESSPGPRLGKEMSEEPFNIWGTWHSTLKSGSKD PGMTGSYGDIFESSSLRPRPCSLSQGNLSLNWPRCQGSPTGLDSGTQVIRRTQTAATVEQCSVHLPVSRV CSTPHIQDGSRGTRRPAASSDPFLSLNSTEDLAEGKEDVAWLQSQARPVYQRPQESGKDDRRPPPPYPGS GKPATTSAQLPLEPPLWRLQRHEEGSETAVEGGQQASGEHQTRPKKLSSAYSLSASEQDKQNLGEASWLD WQRERWQIWELLSTDNPDALPETLV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001287530
ORF Size	2385 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287530.1, NP_001274459.1
RefSeq Size	4147 bp
RefSeq ORF	2388 bp
Locus ID	11856
UniProt ID	O54834
MW	89.1 kDa
Gene Summary	GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Could regulate the interactions of signaling molecules with the actin cytoskeleton. Promotes continuous elongation of cytoplasmic processes during cell motility and simultaneous retraction of the cell body changing the cell morphology (By similarity).[UniProtKB/Swiss-Prot Function]

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