Hdac7 (NM_001204280) Mouse Tagged ORF Clone
CAT#: MR231288
- TrueORF®
Hdac7 (myc-DDK-tagged) - Mouse histone deacetylase 7 (Hdac7), transcript variant 7
"NM_001204280" in other vectors (1)
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CNY 8,650.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 5830434K02Rik; HD7; HD7a; Hdac7a; mFLJ00062 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231288 representing NM_001204280
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGCGGGTGGGCCAGCGGCCCACGGTGGAGCCCCCACCAGAGCCTGCGCTGCTGACCCTGCAAC ACCCCCAACGCCTGCACCGCCATCTCTTCCTGGCAGGCTTACACCAGCAACAGCGCTCAGCCGAGCCCAT GAGGCTCTCCATGGACCCACCAATGCCGGAGCTGCAGGGGGGACAGCAGGAGCAAGAACTTCGGCAACTT CTCAATAAAGACAAGAGCAAGCGAAGTGCCGTAGCCAGCAGTGTGGTCAAGCAGAAGCTGGCTGAAGTGA TCCTGAAGAAACAGCAGGCAGCCCTTGAGAGAACAGTCCATCCCAGCAGCCCCAGTATTCCCTACAGAAC TCTTGAGCCCTTGGACACAGAGGGTGCTGCCCGCTCCGTGCTTAGCAGCTTCCTGCCTCCTGTTCCCAGC CTGCCCACTGAACCCCCGGAACACTTTCCCTTGCGTAAAACAGTGTCTGAACCCAACCTGAAGTTGCGCT ACAAACCCAAGAAATCCCTGGAGAGACGCAAGAATCCCCTGCTCAGGAAGGAGAGTGCCCCGCCCAGCCT TCGGAGGAGGCCTGCCGAGACCCTTGGAGATTCCTCCCCCAGTAGTAGCAGCACACCCGCGTCAGGGTGC AGCTCCCCTAATGACAGCGAGCATGGCCCTAACCCTGCCCTAGGCTCAGAGGCTGATGGTGACCGCAGGA CCCATTCAACTTTAGGCCCTCGGGGTCCTGTACTGGGGAACCCCCATGCTCCCCTCTTCCTGCACCACGG TCTGGAGCCAGAGGCTGGGGGCACCTTACCCTCTCGCCTGCAACCCATTCTCCTGCTGGACCCCTCAGTC TCTCATGCCCCACTGTGGACTGTGCCTGGCCTTGGGCCCTTGCCCTTCCACTTTGCCCAGCCCTTACTGA CCACCGAGCGGCTCTCTGGGTCAGGCCTCCATCGACCACTTAACCGGACCCGCTCAGAGCCCCTGCCCCC CAGCGCCACAGCCTCCCCTCTGCTGGCCCCCCTGCAGCCCCGCCAGGATCGGCTCAAACCTCACGTCCAG CTGATCAAGCCAGCCATCTCCCCTCCCCAGAGGCCTGCCAAGCCCAGTGAGAAGCCCCGACTGCGACAGA TACCCTCGGCTGAGGACCTAGAGACAGATGGTGGGGGAGTGGGACCTATGGCGAATGATGGCCTGGAACA TAGGGAGTCAGGCCGTGGGCCTCCTGAGGGCAGAGGCTCCATTTCTCTGCAGCAGCATCAACAGGTGCCA CCCTGGGAGCAGCAGCATCTAGCCGGGCGGCTCTCTCAGGGAAGCCCCGGGGACTCCGTGCTGATACCTC TGGCCCAGGTTGGACACCGGCCCCTGTCCAGAACCCAGTCTTCCCCAGCAGCACCTGTCTCCCTGCTGAG CCCAGAGCCCACCTGTCAGACCCAAGTCCTCAACAGCTCAGAGACACCTGCTACAGGGCTGGTCTATGAC TCGGTGATGCTGAAACACCAATGTTCCTGTGGAGACAACAGCAAGCATCCCGAGCATGCAGGCCGCATCC AGAGCATCTGGTCCCGGCTGCAGGAACGGGGTCTCCGCAGCCAGTGTGAGTGTCTCCGAGGCCGAAAGGC TTCCCTAGAGGAGCTGCAGTCAGTCCACTCTGAACGGCACGTGCTCCTCTACGGCACGAACCCACTCAGC CGCCTCAAACTGGATAACGGGAAGCTTACAGGACTCCTGGCACAGCGGACGTTTGTGATGCTACCCTGTG GCGGGGTTGGGGTCGATACTGACACCATCTGGAACGAGCTGCATTCCTCCAATGCAGCCCGCTGGGCTGC GGGCAGTGTCACCGACCTTGCCTTCAAAGTAGCTTCCCGAGAGCTGAAGGATGTTCACCATGGCAACGGC ACACAGCAGACTTTCTACCAGGACCCCAGTGTGCTCTACATTTCCCTTCATCGCCATGACGACGGCAACT TCTTCCCAGGCAGTGGGGCCGTGGATGAGGTGGGAACTGGCAGTGGCGAGGGCTTCAATGTCAACGTGGC TTGGGCTGGGGGCTTGGATCCACCCATGGGGGATCCTGAGTACCTGGCTGCTTTCAGGATAGTGGTGATG CCCATTGCCCGAGAGTTTGCTCCAGACCTGGTCCTGGTGTCTGCTGGGTTTGATGCTGCGGAGGGTCACC CAGCCCCGCTGGGTGGCTACCATGTTTCTGCCAAATGTTTTGGGTACATGACGCAGCAGTTGATGAACTT GGCAGGAGGCGCCGTGGTGTTGGCCTTAGAGGGTGGACATGACCTCACGGCCATCTGTGATGCCTCGGAG GCCTGTGTAGCTGCTCTTCTGGGCAACAAGGTGGACCCCCTTTCAGAAGAAAGCTGGAAACAGAAACCCA ACCTCAGTGCCATCCGCTCGCTGGAAGCTGTGGTCAGGGTGCACAGGAAATACTGGGGCTGCATGCAGCG CTTGGCCTCCTGTCCAGACTCCTGGCTACCCAGAGTGCCGGGAGCTGATGCAGAAGTGGAAGCCGTGACC GCGCTGGCATCCCTTTCTGTGGGCATCCTGGCTGAAGACAGGCCCTCGGAGCGGCTGGTGGAAGAGGAAG AACCCATGAACCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231288 representing NM_001204280
Red=Cloning site Green=Tags(s) MDLRVGQRPTVEPPPEPALLTLQHPQRLHRHLFLAGLHQQQRSAEPMRLSMDPPMPELQGGQQEQELRQL LNKDKSKRSAVASSVVKQKLAEVILKKQQAALERTVHPSSPSIPYRTLEPLDTEGAARSVLSSFLPPVPS LPTEPPEHFPLRKTVSEPNLKLRYKPKKSLERRKNPLLRKESAPPSLRRRPAETLGDSSPSSSSTPASGC SSPNDSEHGPNPALGSEADGDRRTHSTLGPRGPVLGNPHAPLFLHHGLEPEAGGTLPSRLQPILLLDPSV SHAPLWTVPGLGPLPFHFAQPLLTTERLSGSGLHRPLNRTRSEPLPPSATASPLLAPLQPRQDRLKPHVQ LIKPAISPPQRPAKPSEKPRLRQIPSAEDLETDGGGVGPMANDGLEHRESGRGPPEGRGSISLQQHQQVP PWEQQHLAGRLSQGSPGDSVLIPLAQVGHRPLSRTQSSPAAPVSLLSPEPTCQTQVLNSSETPATGLVYD SVMLKHQCSCGDNSKHPEHAGRIQSIWSRLQERGLRSQCECLRGRKASLEELQSVHSERHVLLYGTNPLS RLKLDNGKLTGLLAQRTFVMLPCGGVGVDTDTIWNELHSSNAARWAAGSVTDLAFKVASRELKDVHHGNG TQQTFYQDPSVLYISLHRHDDGNFFPGSGAVDEVGTGSGEGFNVNVAWAGGLDPPMGDPEYLAAFRIVVM PIAREFAPDLVLVSAGFDAAEGHPAPLGGYHVSAKCFGYMTQQLMNLAGGAVVLALEGGHDLTAICDASE ACVAALLGNKVDPLSEESWKQKPNLSAIRSLEAVVRVHRKYWGCMQRLASCPDSWLPRVPGADAEVEAVT ALASLSVGILAEDRPSERLVEEEEPMNL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001204280 |
ORF Size | 2604 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001204280.1, NP_001191209.1 |
RefSeq Size | 4084 bp |
RefSeq ORF | 2607 bp |
Locus ID | 56233 |
MW | 94.3 kDa |
Gene Summary | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Positively regulates the transcriptional repressor activity of FOXP3 (By similarity). Serves as a corepressor of RARA, causing its deacetylation and inhibition of RARE DNA element binding (By similarity). In association with RARA, plays a role in the repression of microRNA-10a and thereby in the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229077 | Hdac7 (untagged) - Mouse histone deacetylase 7 (Hdac7), transcript variant 7 |
CNY 8,360.00 |