Arvcf (NM_001272030) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231430 representing NM_001272030 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGACTGCAACGTGCATTCGGCCGCCAGCATTTTGGCCTCGGTGAAGGAACAGGAGGCCCGTTTCG AGCGGCTGACACGGGCACTAGAACAAGAACGGCGCCATGTTGCCCTGCAGCTGGAGCGTGCCCAGCAGCC TGGCATGAGCAGTGGTGGTATGGTGGGCAGTGGGCAGCCCCTGCCAATGGCCTGGCAGCAGCTGGTTCTG CAGGAGCAGAGCCCGGGTAGCCAGGCATCGCTGGCCACGATGCCAGAGGCACCCGAGGTACTGGAGGAGA CAGTGACTGTGGAGGAAGACCCTGGCACCCCCACCTCTCACGTGTCCATCGTCACATCAGAAGATGGTAC AACCCGGCGGACTGAGACTAAGGTCACTAAGACGGTCAAGACTGTGACCACAAGGACAGTACGCCAGGTG CCTTTGGGCCCAGATGGACTCCCCTTGCTGGACGGCGGCCCTCCACTTGGCTCTTTTGCTGACGGGCCCC TGGACCGGCATTACCTACTTCGTGGTGGTGGTGGTCCAGCAGCCACACTCTCCCGAACCTACCACAGCAG TGGAGGTGGCTTTCCCGATGGCCCCGAGTCCCGTGATATTCCAAGCTATGGCAGCTTGTCCCGAGGGCTC GGGGTACGGCCACCACGTACTGGCCTCCTGGGCCCAGGACCTGGTGATGGCTGTTTCACACTGCCTGGCC GCCGAGAAGCCTTCCCCATGGGCTCTGAATCCGGACCACCAAGCGGCCGCTCTCTGCCTGAGCATTTCCA AGCTGAACCATATGGCCTGGAGGATGATACACGGAGCCTGGCTGCGGATGATGAGGGTGGCCCCGACCTG GAGCCTGACTATAGCACGGCAACCCGGAGAAGACCTGAGTACGGGCGGGGCCTTCGTGCCAGGGCCTTTG AAGACACAGCAGATGATGCTGGTGAGCTGATAGAAGAGCGTCCCCCATTTCCAGCAGCAACAGCCCCTCT GGCCCAGCCTGAGAGGGGCAGCCTGGGCAGCTTGGACCGAGTAGTGCGGCGCTCACCTTCAGTGGATAGC ACCCGCAAGGAGCCACGCTGGCGGGACCCCGAGCTGCCAGAGGTGTTGGCCATGCTGCGGCACCCTGTGG ACCCTGTGAAAGCCAATGCTGCAGCCTACCTGCAGCACCTCTGCTTTGAGAACGAGGGTATTAAGCGGCG AGTGCGGCAGCTGCGTGGGCTCCCCCTGCTTGTGGCATTACTAGATCACCCTCGGGCTGAGGTGCGGCGC CGGGCCTGTGGGGCACTGCGCAACCTCTCCTATGGCCGGGATACTGACAACAAGGCCGCCATCCGGGACT GCGGGGGCGTGCCAGCCCTGGTGCGCCTACTGCGGGCGGCCCGTGACAATGAGGTCCGTGAGCTGGTCAC TGGCACACTCTGGAACCTGTCATCCTACGAGCCCCTGAAGATGGTCATCATTGACCACGGCTTACAGACT CTGACCCATGAGGTCATCGTGCCCCATTCCGGCTGGGAGCGAGAGCCTAACGAAGACTCGAAGCCCCGGG ATGCCGAGTGGACAACAGTCTTCAAGAACACATCAGGCTGCCTGAGGAATGTGAGCTCAGATGGTGCAGA GGCCCGGCGGCGACTCCGTGAGTGCGAAGGGCTGGTGGACGCTCTCCTACATGCCCTGCAGTCAGCTGTG GGCAGGAAAGACACAGATAATAAGTCAGTAGAGAACTGTGTGTGCATCATGCGGAACCTGTCCTACCACG TGCACAAAGAAGTTCCAGGGGCTGACAGGTACCAGGAGGCAGAGCCTGGGATCCCGGGCAGCACAACCTC CCAGCGACGGAGGAAGGATGACGCCAGCTGCTTTGGTGGCAAGAAAGCAAAAGGGAAGAAGGATGCAGAG ATGGACCGGAACTTTGACACACTGGACCTGCCTAAACGAACGGAGGCTGCAAAAGGCTTCGAGCTGCTGT ACCAGCCGGAGGTGGTACGTCTCTACCTCTCACTCCTTACGGAGAGCCGGAACTTCAACACCCTGGAAGC TGCAGCCGGTGCCCTGCAAAACCTCAGTGCTGGCAACTGGACGTGGGCCACGTACATCCGTGCCACAGTG CGCAAGGAACGTGGGCTGCCAGTACTGGTGGAACTGCTACAGTCTGAGACCGACAAGGTGGTGCGCGCTG TAGCCATCGCGCTGCGCAACCTCTCACTAGACCAGCGGAACAAAGACCTCATCGGGAGCTATGCCATGAC AGAGCTGGTTCGGAATGTTCGCAATGCACAGGCTCCCGCTCACCCCAGTGCCCACCTGGAGGAGGATACT GTGGTTGCCGTGCTCAACACCATCCATGAGATCGTGTCCGACAGCCTGGACAATGCTCGCTCCCTCCTGC AGGCCCGTGGTGTGCCTGCCCTGGTAGCACTTGTGGCCTCCAGCCAGTCAGTGCGGGAGGCCAAGGCAGC ATCACACGTGCTACAGACTGTATGGAGCTACAAGGAGCTGCGTGGAGCCCTGCAGAGGGATGGCTGGACG AAGTCACGCTTCCAGTCTGCTAGCACTGCCAAAGGACCCAAAGGGACACCAAGTTCTGGCGGCTTTGATG ACAGCACACTGCCACTGGTAGACAAGAGCCTTGATGGGGAGAAGTCAAACACCCGGGATGTGATCCCCAT GGATACCCTTGGTCCAGATGGTTACGCCACAGTTGACCGGAGGGAGAGGAGGACACTGGGCAGTGACTCC ACAGGGGACACCTCTGAGAAAGAACTATTGAGACCCGATCCTGGCAGGAAGGCCCCTCCGCCTGGGCCCA GCAGGCCCTCGGTCAGGCTGGTGGACGCCGTGGGGGACACTAAGCCTCAGCCTGTTGACTCCTGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231430 representing NM_001272030 Red=Cloning site Green=Tags(s) MEDCNVHSAASILASVKEQEARFERLTRALEQERRHVALQLERAQQPGMSSGGMVGSGQPLPMAWQQLVL QEQSPGSQASLATMPEAPEVLEETVTVEEDPGTPTSHVSIVTSEDGTTRRTETKVTKTVKTVTTRTVRQV PLGPDGLPLLDGGPPLGSFADGPLDRHYLLRGGGGPAATLSRTYHSSGGGFPDGPESRDIPSYGSLSRGL GVRPPRTGLLGPGPGDGCFTLPGRREAFPMGSESGPPSGRSLPEHFQAEPYGLEDDTRSLAADDEGGPDL EPDYSTATRRRPEYGRGLRARAFEDTADDAGELIEERPPFPAATAPLAQPERGSLGSLDRVVRRSPSVDS TRKEPRWRDPELPEVLAMLRHPVDPVKANAAAYLQHLCFENEGIKRRVRQLRGLPLLVALLDHPRAEVRR RACGALRNLSYGRDTDNKAAIRDCGGVPALVRLLRAARDNEVRELVTGTLWNLSSYEPLKMVIIDHGLQT LTHEVIVPHSGWEREPNEDSKPRDAEWTTVFKNTSGCLRNVSSDGAEARRRLRECEGLVDALLHALQSAV GRKDTDNKSVENCVCIMRNLSYHVHKEVPGADRYQEAEPGIPGSTTSQRRRKDDASCFGGKKAKGKKDAE MDRNFDTLDLPKRTEAAKGFELLYQPEVVRLYLSLLTESRNFNTLEAAAGALQNLSAGNWTWATYIRATV RKERGLPVLVELLQSETDKVVRAVAIALRNLSLDQRNKDLIGSYAMTELVRNVRNAQAPAHPSAHLEEDT VVAVLNTIHEIVSDSLDNARSLLQARGVPALVALVASSQSVREAKAASHVLQTVWSYKELRGALQRDGWT KSRFQSASTAKGPKGTPSSGGFDDSTLPLVDKSLDGEKSNTRDVIPMDTLGPDGYATVDRRERRTLGSDS TGDTSEKELLRPDPGRKAPPPGPSRPSVRLVDAVGDTKPQPVDSWV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001272030
ORF Size	2868 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001272030.1, NP_001258959.1
RefSeq Size	4569 bp
RefSeq ORF	2871 bp
Locus ID	11877
UniProt ID	P98203
MW	104.7 kDa
Gene Summary	Involved in protein-protein interactions at adherens junctions.[UniProtKB/Swiss-Prot Function]

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