Sema6c (NM_001272024) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA1869; Semay
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231436 representing NM_001272024 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGTGCCCCCCACTCCATGCCCCTGCTGCTCCTGCTGCTGTTGCTGTCATCGCTCCCCCAAGCCC AGGCTGCCTTTCCCCAGGACCCCACCCCTCTGTTGACCTCTGACCTTCAAGGTGCCTCTCCATCATCCTG GTTCCGGGGCCTGGAGGACGATGCTGTGGCTGCGGAACTTGGGCTGGACTTTCAGAGATTCCTGACCTTG AACCGGACCTTGCTTGTGGCTGCCCGGGATCACGTTTTCTCCTTCGATCTTCAAGCCCAAGAAGAAGGGG AGGGGCTGGTGCCCAACAAGTTTCTGACATGGAGGAGTCAAGATATGGAGAACTGTGCAGTCCGGGGAAA GCTGACGGACGAATGCTACAACTACATCCGTGTTCTTGTTCCCTGGAACTCGCAGACACTTCTTGCCTGT GGAACAAATTCCTTCAGCCCTATGTGCCGCAGCTATGGGATAACATCTCTGCAACAGGAGGGTGAGGAGC TGAGTGGGCAGGCTCGATGCCCCTTTGATGCCACCCAGTCCACTGTGGCCATCTTTGCAGAGGGCAGTTT GTACTCAGCCACAGCAGCAGATTTCCAGGCCAGTGATGCTGTAGTTTACAGAAGTCTTGGACCTCAGCCT CCACTCCGTTCTGCAAAGTATGACTCCAAGTGGCTTCGAGAGCCACACTTTGTCTATGCGTTGGAGCATG GAGAGCACGTCTACTTCTTCTTCCGAGAAGTCTCTGTGGAGGATGCCCGCTTAGGGAGGGTGCAGTTTTC CAGGGTAGCCCGGGTATGTAAACGTGACATGGGTGGCTCACCTCGGGCCTTGGATCGCCACTGGACATCC TTCCTTAAGCTGAGGCTCAACTGCTCTGTCCCTGGGGACTCTACCTTCTACTTTGACGTCTTACAGTCCT TAACTGGGCCTGTGAACCTGCATGGCCGCTCCGCTCTCTTTGGGGTCTTCACTACTCAGACCAATAGCAT TCCTGGGTCTGCAGTCTGCGCCTTCTACCTAGATGATATTGAACGTGGCTTTGAGGGCAAGTTCAAGGAG CAGAGGAGTCTGGATGGGGCCTGGACTCCTGTGTCTGAGGACAAGGTCCCCTCACCCAGGCCAGGGTCCT GTGCAGGTGTGGGTGCAGCTGCCTCGTTCTCCTCCTCTCAAGACCTACCTGATGATGTCCTGCTCTTCAT CAAGGCACACCCGCTGCTGGATCCTGCTGTGCCACCCGCCACCCATCAACCGCTCCTCACTCTGACTAGC AGGGCCCTGCTGACCCAGGTAGCTGTGGACGGAATGGCTGGTCCCCACAGAAATACTACTGTCCTGTTTC TTGGCTCCAACGATGGGACAGTGCTGAAGGTGCTACCTCCAGGGGGTCAGTCTTTGGGCTCTGAGCCTAT CGTCTTGGAAGAGATTGATGCCTACAGCCATGCCCGGTGCAGTGGGAAGCGGTCACCCCGAGCTGCGCGG CGGATCATAGGGCTGGAGCTGGACACTGAGGGTCACAGGCTTTTTGTGGCCTTTCCTGGATGTATCGTCT ACCTCTCTCTCAGCCGGTGTGCCCGGCATGGAGCATGTCAGAGGAGCTGCCTGGCTTCTCTGGACCCATA CTGTGGATGGCATCGATCCAGAGGCTGCATGAGTATCAGGGGACCTGGTGGGACTGATGTCGATCTGACT GGGAACCAGGAATCCACAGAGCACGGTGACTGCCAAGATGGAGCGACCGGGAGTCAGTCTGGCCCTGGAG ATTCTGCTTATGTGCTTCTGGGTCCTGGCCCTTCCCCTGAGACCCCCAGTTCCCCCAGTGATGCCCAACA AGGGCCCCAGTCTTCCACTCTTGGAGCTCACACACAGGGCGTGCGCAGGGACCTTTCCCCAGCCTCAGCC TCCCGATCCATTCCCATCCCACTCCTCCTGGCCTGTGTGGCCGCGGCCTTCGCTTTGGGCGCCTCAGTCT CCGGTCTCTTGGTGTCCTGTGCTTGTCGTCGCGCGAACCGCCGTCGGAGCAAGGACATCGAGACCCCGGG GCTGCCGCGCCCCCTCTCCCTTCGCAGCCTGGCCCGGCTGCACGGTGGCGGTCCTGAGCCCCCGCCTCCG CCCAAGGATGGAGATGCAGCGCAAACGCCCCAGCTCTACACTACCTTCCTGCCTCCGCCCGACGGCGGAT CCCCACCGGAGCTGGCCTGCCTACCCACGCCGGAGACGACGCCCGAGCTGCCCGTGAAGCACCTCCGTGC CTCCGGGGGCCCCTGGGAATGGAACCAGAATGGGAACAACGCCTCGGAGGGCCCAGGCCGCCCACCACGG GGCTGCAGCGGGGCGGGCGGGCCCGCACCGCGAGTGCTGGTGAGGCCACCGCCCCCTGGCTGCCCCGGGC AGGCGGTAGAGGTGACCACGCTGGAGGAACTGCTGCGCTACCTGCACGGCCCTCAGCCGCCCAGGAAGGG TAGCGAACCTCTCGCCTCCGCCCCGTTCACCTCCCGGCCGCCCGCCTCGGAGCCCGGCGCCTCGCTGTTC GTGGACTCCAGCCCGATGCCGCGGGATGGCGTTCCGCCGCTCAGGCTCGACGTGCCACCCGAAGGCAAGC GCGCTGCCCCGAGCGGGCGGCCTGCTCTCTCGGCCCCAGCCCCGCGCCTGGGTGTCGGCGGCAGCCGCCG ATTGCCCTTTCCCACACACCGGGCGCCCCCGGGCCTGCTCACGCGAGTGCCCTCGGGAGGCCCGGCCAGG TACTCCGGGGGGCCCGGGAGGCACCTCCTGTACCTGGGCCGGCCCGAAGGCCATCGCGGCCGCTCCCTGA AGAGGGTGGACGTGAAGTCTCCGCTGTCGCCCAAGCCGCCCCTCGCCTCCCCGCCGCAGCCCGCCCCGCA CGGTGGTCATTTCAACTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231436 representing NM_001272024 Red=Cloning site Green=Tags(s) MPRAPHSMPLLLLLLLLSSLPQAQAAFPQDPTPLLTSDLQGASPSSWFRGLEDDAVAAELGLDFQRFLTL NRTLLVAARDHVFSFDLQAQEEGEGLVPNKFLTWRSQDMENCAVRGKLTDECYNYIRVLVPWNSQTLLAC GTNSFSPMCRSYGITSLQQEGEELSGQARCPFDATQSTVAIFAEGSLYSATAADFQASDAVVYRSLGPQP PLRSAKYDSKWLREPHFVYALEHGEHVYFFFREVSVEDARLGRVQFSRVARVCKRDMGGSPRALDRHWTS FLKLRLNCSVPGDSTFYFDVLQSLTGPVNLHGRSALFGVFTTQTNSIPGSAVCAFYLDDIERGFEGKFKE QRSLDGAWTPVSEDKVPSPRPGSCAGVGAAASFSSSQDLPDDVLLFIKAHPLLDPAVPPATHQPLLTLTS RALLTQVAVDGMAGPHRNTTVLFLGSNDGTVLKVLPPGGQSLGSEPIVLEEIDAYSHARCSGKRSPRAAR RIIGLELDTEGHRLFVAFPGCIVYLSLSRCARHGACQRSCLASLDPYCGWHRSRGCMSIRGPGGTDVDLT GNQESTEHGDCQDGATGSQSGPGDSAYVLLGPGPSPETPSSPSDAQQGPQSSTLGAHTQGVRRDLSPASA SRSIPIPLLLACVAAAFALGASVSGLLVSCACRRANRRRSKDIETPGLPRPLSLRSLARLHGGGPEPPPP PKDGDAAQTPQLYTTFLPPPDGGSPPELACLPTPETTPELPVKHLRASGGPWEWNQNGNNASEGPGRPPR GCSGAGGPAPRVLVRPPPPGCPGQAVEVTTLEELLRYLHGPQPPRKGSEPLASAPFTSRPPASEPGASLF VDSSPMPRDGVPPLRLDVPPEGKRAAPSGRPALSAPAPRLGVGGSRRLPFPTHRAPPGLLTRVPSGGPAR YSGGPGRHLLYLGRPEGHRGRSLKRVDVKSPLSPKPPLASPPQPAPHGGHFNF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001272024
ORF Size	2889 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001272024.1, NP_001258953.1
RefSeq Size	4140 bp
RefSeq ORF	2892 bp
Locus ID	20360
UniProt ID	Q9WTM3
MW	103.1 kDa
Gene Summary	May be a stop signal for the dorsal root ganglion neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections (By similarity).[UniProtKB/Swiss-Prot Function]

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