Opa1 (NM_001199177) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231457 representing NM_001199177 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGAGCAGGTCGGGCGGCCGTGGCCTGTGAAGTCTGCCAATCCTTAGTGAAACACAGTTCTGGAA TACAAAGAAACGTACCGCTCCAAAAACTCCATCTGGTTTCACGAAGTATTTATCGTTCACATCATCCTGC CCTCAAGCTTCAAAGACCCCAACTAAGGACACCATTTCAGCAGTTCTCTTCTCTAACTCACCTTTCATTA CATAAATTGAAACTTTCTCCAATTAAATATGGCTACCAGCCCCGCAGGAACTTTTGGCCAGCAAGGTTAG CTGCAAGACTCTTAAAACTTCGATATATCATACTGGGATCTGCTGTTGGAGGTGGCTATACAGCCAAAAA AACCTTCGATGAATGGAAAGATATGATACCAGACCTTAGTGACTATAAGTGGATTGTGCCTGACTTTATA TGGGAAATTGATGAGTATATTGATTTGGAGAAAATTAGAAAAGCCCTGCCCAGCTCAGAAGACCTTGCCA GTTTAGCTCCCGACCTGGACAAGATTACTGAGAGCCTCAGCTTGTTGAAGGACTTCTTCACTGCAGGTCC CAAATTGGTTAGTGAAGTCCTAGAAGTTTCTGAGGCCCTTCTCTTGTTAGGTTCACCTGGAGAAACAGCA TTTCGAGCAACAGATCATGGATCTGAAAGTGACAAGCATTACAGGAAGGTGTCAGACAAAGAAAAGATTG ACCAACTTCAAGAAGAACTTCTGCATACTCAGTTAAAGTATCAGAGGATCTTGGAGCGCCTGGAAAAGGA GAACAAAGAGCTGCGGAAGCTGGTGCTGCAGAAGGACGACAAAGGCATCCACCACAGGAAGCTCAAGAAA TCTTTGATTGATATGTATTCTGAAGTTCTTGATGTTCTTTCTGATTATGATGCCAGTTACAATACACAAG ATCACCTACCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGGAAAACCAGTGTGCTGGAAATGATTGC TCAGGCCCGGATCTTCCCGAGAGGGTCCGGCGAGATGATGACACGCTCTCCAGTGAAGGTGACTCTCAGT GAAGGCCCTCACCATGTGGCCTTGTTTAAAGATAGCTCTCGGGAATTTGATCTCACCAAGGAGGAAGATC TTGCAGCATTAAGACATGAAATCGAACTCCGAATGAGGAAAAATGTGAAAGAAGGTTGTACTGTTAGCCC CGAGACCATATCTCTAAATGTCAAAGGCCCTGGGCTGCAGAGGATGGTGCTCGTGGACTTGCCTGGTGTC ATCAACACCGTGACATCAGGCATGGCTCCCGACACAAAGGAAACTATTTTCAGTATCAGCAAAGCTTACA TGCAGAATCCTAACGCCATCATCCTGTGCATCCAAGACGGATCCGTAGATGCTGAGCGCAGTATTGTTAC AGACTTGGTCAGTCAAATGGATCCTCATGGAAGAAGAACCATATTTGTTTTGACCAAAGTAGACCTGGCA GAAAAAAATGTAGCCAGTCCAAGCAGGATACAACAGATAATTGAAGGCAAGCTCTTCCCAATGAAAGCTC TGGGTTATTTTGCTGTCGTAACAGGAAAAGGAAACAGCTCTGAAAGTATTGAAGCTATAAGAGAGTATGA AGAGGAATTTTTTCAGAATTCAAAACTGCTAAAGACAAGCATGCTAAAGGCACACCAGGTCACCACGAGA AATCTCAGCCTTGCTGTGTCAGACTGCTTTTGGAAAATGGTTCGAGAGTCAGTTGAACAACAGGCTGATA GTTTTAAAGCCACGCGCTTTAACCTAGAGACGGAATGGAAGAATAACTACCCGCGCCTGCGAGAGCTCGA CAGGAATGAACTCTTTGAAAAAGCTAAAAATGAGATCCTCGATGAGGTCATCAGTCTGAGCCAGGTCACG CCAAAGCACTGGGAGGAAATCCTGCAGCAATCCCTGTGGGAACGAGTGTCAACACATGTGATTGAGAACA TCTACCTTCCAGCTGCCCAGACCATGAATTCGGGAACATTTAACACCACAGTAGACATCAAGCTTAAACA GTGGACTGACAAGCAGCTTCCTAATAAAGCAGTCGAGGTTGCCTGGGAGACTCTACAAGAGGAATTTTCC CGCTTCATGACAGAACCCAAAGGAAAGGAACACGACGACATATTTGACAAACTTAAGGAGGCTGTGAAGG AGGAGAGTATCAAGCGGCACAAGTGGAACGACTTTGCCGAGGATAGCTTGAGGGTTATTCAGCACAATGC TTTGGAAGACCGGTCCATATCAGATAAGCAACAGTGGGATGCAGCCATTTACTTCATGGAAGAGGCGCTT CAAGGTCGTCTCAAGGATACTGAAAATGCTATTGAAAACATGATTGGGCCAGACTGGAAAAAGAGGTGGA TGTACTGGAAGAATCGGACCCAAGAGCAGTGTGTTCACAACGAAACCAAGAACGAGTTGGAGAAGATGCT GAAGGTTAATGATGAGCACCCAGCTTACCTGGCAAGTGATGAGATTACCACAGTCCGGAAGAACCTGGAG TCTCGAGGAGTGGAAGTCGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTTTATAGAAGACATTTCT TAAAAACAGCTCTAAATCATTGTAACCTTTGTCGCAGAGGTTTTTATTACTACCAGAGGCATTTTATAGA TTCTGAGCTGGAATGCAATGACGTGGTCCTGTTTTGGCGAATACAGCGCATGCTCGCTATCACTGCCAAT ACATTAAGGCAGCAGCTTACAAACACTGAAGTTAGGCGACTAGAGAAAAACGTTAAAGAGGTATTAGAAG ATTTTGCAGAAGACGGTGAGAAGAAGGTTAAATTGCTCACTGGCAAACGAGTTCAGCTGGCAGAAGATCT CAAGAAAGTTAGAGAAATTCAAGAAAAGCTTGATGCTTTCATTGAAGCTCTTCACCAGGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231457 representing NM_001199177 Red=Cloning site Green=Tags(s) MWRAGRAAVACEVCQSLVKHSSGIQRNVPLQKLHLVSRSIYRSHHPALKLQRPQLRTPFQQFSSLTHLSL HKLKLSPIKYGYQPRRNFWPARLAARLLKLRYIILGSAVGGGYTAKKTFDEWKDMIPDLSDYKWIVPDFI WEIDEYIDLEKIRKALPSSEDLASLAPDLDKITESLSLLKDFFTAGPKLVSEVLEVSEALLLLGSPGETA FRATDHGSESDKHYRKVSDKEKIDQLQEELLHTQLKYQRILERLEKENKELRKLVLQKDDKGIHHRKLKK SLIDMYSEVLDVLSDYDASYNTQDHLPRVVVVGDQSAGKTSVLEMIAQARIFPRGSGEMMTRSPVKVTLS EGPHHVALFKDSSREFDLTKEEDLAALRHEIELRMRKNVKEGCTVSPETISLNVKGPGLQRMVLVDLPGV INTVTSGMAPDTKETIFSISKAYMQNPNAIILCIQDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLA EKNVASPSRIQQIIEGKLFPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSMLKAHQVTTR NLSLAVSDCFWKMVRESVEQQADSFKATRFNLETEWKNNYPRLRELDRNELFEKAKNEILDEVISLSQVT PKHWEEILQQSLWERVSTHVIENIYLPAAQTMNSGTFNTTVDIKLKQWTDKQLPNKAVEVAWETLQEEFS RFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAEDSLRVIQHNALEDRSISDKQQWDAAIYFMEEAL QGRLKDTENAIENMIGPDWKKRWMYWKNRTQEQCVHNETKNELEKMLKVNDEHPAYLASDEITTVRKNLE SRGVEVDPSLIKDTWHQVYRRHFLKTALNHCNLCRRGFYYYQRHFIDSELECNDVVLFWRIQRMLAITAN TLRQQLTNTEVRRLEKNVKEVLEDFAEDGEKKVKLLTGKRVQLAEDLKKVREIQEKLDAFIEALHQEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199177
ORF Size	2934 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199177.1, NP_001186106.1
RefSeq Size	6002 bp
RefSeq ORF	2937 bp
Locus ID	74143
MW	113.7 kDa
Gene Summary	Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function]

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