Ncoa6 (NM_001242558) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AIB3; ASC-2; ASC2; mKIAA0181; Ncoa7; NRC; PRIP; RAP250
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231556 representing NM_001242558 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTTTGGATGACCTTCCAAACTTTGAAGACATCTATACTTCCTTGTGTTCATCTACAATGGGCGACT CAGAGGTGGAGTTTGACTCTGGACTAGAAGATGATGACACGAAAAGTGATAGTATTTTGGAGGATTCCAC AATTTTTGTAGCCTTCAAAGGAAATATAGATGATAAAGACTTCAAATGGAAACTGGATGCAATATTAAAG AATGTGCCCAACTTGTTACACATGGAATCCAGCAAGCTAAAGGTACAGAAGGTGGAGCCCTGGAACAGCG TGCGAGTCACATTCAACATCCCCCGGGAAGCAGCAGAACGGCTACGGATCCTGGCTCAGAGCAACAACCA GCAGCTTCGGGACCTGGGGATTCTCTCCGTTCAGATTGAAGGGGAAGGTGCTATCAACCTGGCTTTGGGT CAGAACCGAAGCCAAGATGTGAGAATGAATGGACCTGTGGCATCTGGAAATTCCGTTAGGATGGAGGCAG GATTTCCCATGGCAAGTGGTCCAGGTCTAATAAGGATGACGAGCCCTGCCGCTGTTATGACGCCCCAGGG CGGGAACATGTCATCTTCCATGATGGCACCAGGCCCCAATCCAGAACTGCAACCCAGGACTCCTCGCCCT GCTTCTCAGTCAGATGCAATGGATCCACTTCTCTCTGGACTCCATATACAGCAACAGAGTCATCCCTCAG GATCTTTACCTCCAGCGCATCACTCAATGCAGCCTGTTCCTGTGAACAGACAAATGAACCCAGCTAATTT TCCCCAGCTGCAGCAGCAGCAGCAGCAACAGCAGCAGCAACAGCAACAGCAGCAACAGCAACAGCAGCAA CAGCAACAGCAGCAGCAACAACAGCAGCAGCAACAGTTGCAGACAAGACCTCTACAGCAACATCAGCAAC AGCCACAGGGGATTCGACCACAGTTTACTGCTCCAACTCAGGTGCCTGTTCCTCCAGGCTGGAACCAGCT GCCTTCTGGAGCCTTACAGCCTCCACCAGCCCAGGGATCTCTGGGCACAATGACTACAAATCAAGGGTGG AAGAAGGCTCCCTTGCCTAGCCCAATGCAAGCGCAACTTCAGGCAAGACCTTCCTTAGCTACGGTACAGA CACCTTCTCACCCTCCCCCTCCTTATCCTTTTGGCAGCCAACAAGCCTCACAAGCCCATACAAACTTTCC TCAAATGAGCAACCCAGGCCAGTTCACAGCTCCTCAGATGAAGGGCTTGCAGGGAGGGCCCTCCAGGGTC CCAACCCCCCTGCAACAGCCCCACCTCACCAACAAGTCTCCTGCCTCCTCACCCTCCTCCTTTCAGCAGG GATCTCCTGCATCCTCCCCAACGGTTAACCAAACTCAGCAGCAGATGGGACCAAGGCCACCTCAAAATAA CCCACTTTCCCAGGGATTTCAGCAGCCTGTCAGCTCTCCAGGTCGGAATCCTATGGTTCAACAGGGAAAT GTGCCACCTAATTTCATGGTGATGCAGCAGCAACCACCAAACCAGGGGCCACAGAGTTTACATCCAGGCC TAGGAGGAATGCCTAAACGCCTCCCGCCTGGCTTCTCAGCAGGACAGGCCAATCCCAACTTCATGCAAGG TCAGGTGCCTTCCACCACAGCAGCCACCCCTGGGAATTCAGGAGCCCTTCAGCTGCAAGCAAATCAAAAT GTCCAGCACGCAGGTGGTCAAGGAGCTGGTCCTCCTCAAAACCAGATGCAGGTGTCTCACGGGCCACCAA ATATGATGCAACCCAGCCTCATGGGAATTCATGGCAACATAAACAACCAGCAGGCTGGTAGCTCTGGGGT TCCTCAGGTGACCCTGGGCAACATGCAAGGCCAGCCCCAGCAAGGCCCACCATCTCAGCTGATGGGCATG CACCAACAGATTGTGCCCTCACAGGGCCAAATGGCCCAGCAACAAGGAACTTTGAACCCTCAAAATCCTA TGATCCTTTCAAGGGCCCAGCTTATGCCACAGGGCCAGATGATGGTGAACGCTCAAAACCAAAATCTTGG ACCTTCACCCCAAAGGATGACCCCACCCAAGCAGATGCTTCCCCAGCAGGGCCCACAAATGATGGCACCA CATAACCAGATGATGGGGCCTCAGGGGCAAGTTTTGCTCCAGCAGAACCCAATGATAGAGCAAATAATGA CCAATCAGATGCAGGGGAATAAGGCGCAATTTAACTCTCAGAACCAATCCAATGTCATGCCGGGACCAGC ACAAATAATGAGGGGACCAACTCCGAATATGCAAGGAAACATGGTGCAATTCACAGGACAGATGTCAGGA CAGATGCTGCCTCAGCAAGGACCTGTGAACAACAGTCCATCTCAGGTTATGGGGATTCAGGGGCAGGTTC TGCGGCCACCAGGACCCAGTCCACACATGGCCCAGCAACATAATGATCCAGTTACCACAGCAAATAATGA TGTCAACCTGTCTCAGATGATGCCTGATGTCAGCATGCAGCAAGCCAGCATGGTCCCCCCACATGTGCAG AGCATGCAGGGAAACAGTGCTTCGGGAAGCCACTTCTCGGGCCATGGAGTGTCTTTCAATGCACCATTTG GTGGTGCACCCAATGGAAGTCAGATGTCTTGTGGTCAAAATCCAGGCTTTCCCGTCAATAAAGATGTAAC GTTAACGAGCCCATTGTTGGTCAACTTACTACAGAGTGACATTTCTGCAGGCCATTTTGGTGTAAACAAT AAACAAAATAATACCAATGCAAATAAACCGAAGAAGAAGAAACCGCCTCGGAAGAAGAAAAATTGTCACC AAGATCTAAACACCCCAGATAATCGTCCAACTGGTCTAGAGGAAGTTGATCAACAGTCATTACCTGGAGA ACAAGGAATCAACTTGGACACCACAGGCCCCAAACTGCCAGACTTTTCAAACCGGCCACCAGCCCCTTCA CAGAATTTAGCCCCAAAGGAAACTCCAGCCACAGCACTGCAAGGGTCTGTTGCTAGACCAGAACTTGAGG CAAATGCTGCCATAGCCTCTGGACAAAGCTGTGAGCCAAAAGAGATAGTTGAGAAGTCCAAAACCCTGAC GAGCCGAAGAAACTCTCGAACTGAGGAGCCAACTATGGCTTCTGAGAGCGTGGAAAATGGACATCGAAAA AGATCCTCTCGACCTGCTTCGGCCTCCAGCTCTACTAAAGATATAACTGGTGCCGTGCAATCCAAGCGAA GAAAATCCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231556 representing NM_001242558 Red=Cloning site Green=Tags(s) MVLDDLPNFEDIYTSLCSSTMGDSEVEFDSGLEDDDTKSDSILEDSTIFVAFKGNIDDKDFKWKLDAILK NVPNLLHMESSKLKVQKVEPWNSVRVTFNIPREAAERLRILAQSNNQQLRDLGILSVQIEGEGAINLALG QNRSQDVRMNGPVASGNSVRMEAGFPMASGPGLIRMTSPAAVMTPQGGNMSSSMMAPGPNPELQPRTPRP ASQSDAMDPLLSGLHIQQQSHPSGSLPPAHHSMQPVPVNRQMNPANFPQLQQQQQQQQQQQQQQQQQQQQ QQQQQQQQQQQQLQTRPLQQHQQQPQGIRPQFTAPTQVPVPPGWNQLPSGALQPPPAQGSLGTMTTNQGW KKAPLPSPMQAQLQARPSLATVQTPSHPPPPYPFGSQQASQAHTNFPQMSNPGQFTAPQMKGLQGGPSRV PTPLQQPHLTNKSPASSPSSFQQGSPASSPTVNQTQQQMGPRPPQNNPLSQGFQQPVSSPGRNPMVQQGN VPPNFMVMQQQPPNQGPQSLHPGLGGMPKRLPPGFSAGQANPNFMQGQVPSTTAATPGNSGALQLQANQN VQHAGGQGAGPPQNQMQVSHGPPNMMQPSLMGIHGNINNQQAGSSGVPQVTLGNMQGQPQQGPPSQLMGM HQQIVPSQGQMAQQQGTLNPQNPMILSRAQLMPQGQMMVNAQNQNLGPSPQRMTPPKQMLPQQGPQMMAP HNQMMGPQGQVLLQQNPMIEQIMTNQMQGNKAQFNSQNQSNVMPGPAQIMRGPTPNMQGNMVQFTGQMSG QMLPQQGPVNNSPSQVMGIQGQVLRPPGPSPHMAQQHNDPVTTANNDVNLSQMMPDVSMQQASMVPPHVQ SMQGNSASGSHFSGHGVSFNAPFGGAPNGSQMSCGQNPGFPVNKDVTLTSPLLVNLLQSDISAGHFGVNN KQNNTNANKPKKKKPPRKKKNCHQDLNTPDNRPTGLEEVDQQSLPGEQGINLDTTGPKLPDFSNRPPAPS QNLAPKETPATALQGSVARPELEANAAIASGQSCEPKEIVEKSKTLTSRRNSRTEEPTMASESVENGHRK RSSRPASASSSTKDITGAVQSKRRKSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242558
ORF Size	3231 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242558.1, NP_001229487.1
RefSeq Size	3896 bp
RefSeq ORF	3234 bp
Locus ID	56406
MW	116.5 kDa

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