Cp (NM_001276250) Mouse Tagged ORF Clone
CAT#: MR231561
- TrueORF®
Cp (myc-DDK-tagged) - Mouse ceruloplasmin (Cp), transcript variant 4
"NM_001276250" in other vectors (1)
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CNY 10,830.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | D3Ertd555; D3Ertd555e |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231561 representing NM_001276250
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTTTTTGCTGCTTAGCACATTTATATTTTTGTATAGTTCCTTAGCCTTGGCAAGAGATAAGCATT ATTTCATTGGAATTACTGAAGCAGTCTGGGACTATGCTTCTGGCACTGAAGAAAAGAAACTTATTTCAGT TGACACGGAACAGTCCAATTTCTATCTTCAAAATGGTCCAGATCGTATTGGAAGAAAATATAAGAAGGCC CTTTATTTTGAGTACACAGATGGCACCTTTAGTAAGACTATAGACAAACCAGCCTGGCTAGGGTTTTTAG GCCCTGTCATCAAAGCTGAAGTTGAAGATAAAGTTTATGTTCACTTAAAGAACCTTGCCTCTAGGATCTA CACTTTTCATGCACATGGGGTAACGTACACCAAGGAGTATGAGGGAGCCGTCTACCCTGACAACACCACT GATTTTCAACGGGCTGATGACAAAGTGCTTCCCGGACAACAGTATGTGTATGTGCTGCATGCCAATGAGC CAAGTCCTGGAGAGGGAGACAGCAATTGTGTGACCAGGATTTACCACTCCCATGTTGATGCTCCAAAAGA TATTGCATCAGGACTCATAGGACCTCTAATACTCTGTAAAAAAGGTTCTCTATATAAGGAAAAAGAGAAA AATATTGACCAAGAATTTGTACTAATGTTCTCTGTGGTGGATGAAAATCTCAGCTGGTATCTGGAAGATA ACATCAAAACCTTCTGCTCTGAACCCGAGAAAGTTGATAAAGACAATGAAGACTTCCAGGAAAGCAACAG GATGTACTCTATAAATGGATATACATTTGGAAGCCTCCCAGGGCTCTCGATGTGTGCAGCAGACAGAGTG AAGTGGTACCTTTTTGGTATGGGTAATGAAGTTGATGTGCATTCAGCTTTCTTTCATGGCCAAGCCCTGA CCAGCAGGAACTATCAAACCGATATAATCAACCTGTTCCCTGCCACCCTAATTGATGCTTATATGGTGGC CCAGAATCCTGGAGTCTGGATGCTCAGTTGCCAGAACCTAAACCATCTGAAAGCTGGGTTGCAGGCCTTT TTCCAGGTTCGTGACTGTAACAAGCCCTCGCCAGAGGATAATATCCAAGATAGGCATGTGAGACACTATT ACATTGCTGCTGAGGAGGTCATCTGGAATTATGCTCCTTCTGGGACGGACATCTTCACTGGAGAGAATTT AACAGCTCTGGAAAGTGATTCAAGGGTATTTTTTGAGCAAGGTGCCACAAGAATTGGTGGCTCTTATAAA AAAATGGCATATCGTGAGTACACAGATGGTTCCTTCACAAACCGAAAACAAAGAGGCCCTGATGAGGAAC ATCTTGGAATCCTAGGTCCTGTCATTTGGGCAGAAGTAGGAGACACCATTAAAGTCACCTTTCATAACAA AGGACAGCATCCTCTCAGCATTCAGCCAATGGGAGTAAGTTTCACTGCAGAAAATGAGGGAACATACTAT GGCCCACCAGGTCGCTCCTCACAGCAAGCCTCCCATGTGGCTCCCAAAGAAACCTTTACATACGAATGGA CTGTCCCCAAAGAAATGGGACCCACTTATGCAGATCCTGTGTGCCTATCTAAGATGTACTACTCTGGCGT TGACCCCACCAAAGATATATTTACTGGGCTTATTGGGCCAATGAAAATATGCAAGAAAGGCAGCTTACTT GCTGATGGGAGACAGAAAGATGTAGACAAAGAGTTCTACTTGTTTCCCACAGTGTTTGATGAGAATGAGA GTTTACTCTTAGATGATAATATCAGGATGTTCACAACTGCACCTGATCAAGTGGATAAGGAAGATGAAGA CTTTCAGGAGTCTAATAAGATGCACTCCATGAATGGGTTCATGTATGGCAATCAGCCTGGCCTCAATATG TGTCTAGGAGAATCCATCGTGTGGTATTTGTTCAGCGCTGGAAATGAGGCTGATGTGCATGGGATATACT TTTCAGGAAATACTTATCTGTCTAAAGGAGAAAGAAGAGACACTGCAAACCTATTCCCTCATAAAAGTCT CACCCTTCTCATGAACCCTGACACAAAAGGGACTTTTGATGTTGAGTGCCTTACAACGGATCACTACACA GGTGGCATGAAGCAAAAATACACTGTGAACCAGTGCCAGCGGCAGTTTGAAGATTTCACTGTCTACCTTG GAGAAAGGACCTACTATGTGGCAGCCGTAGAGGTGGAATGGGATTACTCACCAAGCAGGGCCTGGGAAAA GGAGCTGCATCATTTGCAAGAGCAAAATGTTTCAAATGTATTTTTGGATAAAGAAGAGTTTTTCATAGGC TCAAAGTACAAGAAGGTTGTGTATCGCCAGTTTACTGACAGCTCATTCAGAGAACAGGTGAAGAGACGAG CCGAAGACGAGCACTTGGGCATCCTTGGGCCACCAATTCATGCAAATGTTGGAGACAAAGTTAAAGTTGT CTTTAAAAATATGGCAACCAGGCCATATTCAATACATGCCCATGGGGTGAAAACAGAGAGTTCTACAGTT GTTCCAACGTTACCAGGTGAAGTTCGAACTTATACATGGCAAATTCCAGAAAGATCAGGGGCTGGAAGAG AGGATTCAGCTTGTATCCCATGGGCTTATTACTCAACTGTGGATCGAGTTAAGGATCTCTATAGTGGGCT AATAGGCCCATTGATTGTTTGTCGGAAGTCTTATGTGAAAGTATTCAGTCCTAAAAAGAAAATGGAGTTT TTCCTTCTGTTTCTAGTATTTGATGAGAATGAATCTTGGTACTTAGATGATAACATCAAAACATACTCTG AACACCCTGAGAAAGTAAACAAAGACAACGAGGAATTCCTAGAAAGCAATAAAATGCATGCTATTAATGG GAAAATGTTTGGAAACCTACAAGGCCTCACAATGCACGTGAAAGATGAAGTCAACTGGTATGTGATGGGA ATGGGCAATGAAATAGACCTGCACACTGTACACTTCCACGGCCACAGCTTCCAATACAAGCACAGGGGAG TATACAGTTCTGATGTCTTTGACCTTTTCCCTGGAACATACCAAACCCTAGAAATGTTTCCCCAAACACC TGGAACCTGGTTACTCCACTGCCACGTGACTGACCATGTCCATGCTGGGATGGCAACTACCTACACTGTT TTACCAGTAGAACAAGTATCATCTCAGAGTTACAGGATGACCTGGAACATCCTCTATACACTACTAATCA GCATCATTATTTTATTCCAAATGTCTACCAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231561 representing NM_001276250
Red=Cloning site Green=Tags(s) MKFLLLSTFIFLYSSLALARDKHYFIGITEAVWDYASGTEEKKLISVDTEQSNFYLQNGPDRIGRKYKKA LYFEYTDGTFSKTIDKPAWLGFLGPVIKAEVEDKVYVHLKNLASRIYTFHAHGVTYTKEYEGAVYPDNTT DFQRADDKVLPGQQYVYVLHANEPSPGEGDSNCVTRIYHSHVDAPKDIASGLIGPLILCKKGSLYKEKEK NIDQEFVLMFSVVDENLSWYLEDNIKTFCSEPEKVDKDNEDFQESNRMYSINGYTFGSLPGLSMCAADRV KWYLFGMGNEVDVHSAFFHGQALTSRNYQTDIINLFPATLIDAYMVAQNPGVWMLSCQNLNHLKAGLQAF FQVRDCNKPSPEDNIQDRHVRHYYIAAEEVIWNYAPSGTDIFTGENLTALESDSRVFFEQGATRIGGSYK KMAYREYTDGSFTNRKQRGPDEEHLGILGPVIWAEVGDTIKVTFHNKGQHPLSIQPMGVSFTAENEGTYY GPPGRSSQQASHVAPKETFTYEWTVPKEMGPTYADPVCLSKMYYSGVDPTKDIFTGLIGPMKICKKGSLL ADGRQKDVDKEFYLFPTVFDENESLLLDDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLNM CLGESIVWYLFSAGNEADVHGIYFSGNTYLSKGERRDTANLFPHKSLTLLMNPDTKGTFDVECLTTDHYT GGMKQKYTVNQCQRQFEDFTVYLGERTYYVAAVEVEWDYSPSRAWEKELHHLQEQNVSNVFLDKEEFFIG SKYKKVVYRQFTDSSFREQVKRRAEDEHLGILGPPIHANVGDKVKVVFKNMATRPYSIHAHGVKTESSTV VPTLPGEVRTYTWQIPERSGAGREDSACIPWAYYSTVDRVKDLYSGLIGPLIVCRKSYVKVFSPKKKMEF FLLFLVFDENESWYLDDNIKTYSEHPEKVNKDNEEFLESNKMHAINGKMFGNLQGLTMHVKDEVNWYVMG MGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDLFPGTYQTLEMFPQTPGTWLLHCHVTDHVHAGMATTYTV LPVEQVSSQSYRMTWNILYTLLISIIILFQMSTKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001276250 |
ORF Size | 3255 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001276250.1, NP_001263179.1 |
RefSeq Size | 4712 bp |
RefSeq ORF | 3258 bp |
Locus ID | 12870 |
MW | 124.6 kDa |
Gene Summary | The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229350 | Cp (untagged) - Mouse ceruloplasmin (Cp), transcript variant 4 |
CNY 10,450.00 |