Slc12a4 (NM_001253804) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AW546649; KCC1; RBCKCC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231563 representing NM_001253804 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCACTTCACCGTGGTGCCGGTGGACGGGCCGCGACGCGGCGACTATGACAACCTCGAGGGGCTCA GTTGGGTGGACTACGGCGAGCGCGCCGAGCGGGAAGACTCGGACGGTGAGGGACAGGGTAACCACAGAGA GAATAGCCCTTTCCTTTGCCCTTTGGACGCCTCCAGAGGAAATGACTACTATGACCGGAACTTGGCACTG TTTGAGGAGGAGCTGGACATCCGCCCAAAGGTGTCATCTCTCCTGGGCAAGCTTGTCAGCTATACCAACC TCACCCAAGGAGCCAAGGAGCACGAGGAGGCTGAGAGTGGAGAAGGTGGCCGACGGAGAGCTGCCAAGGC TCCCAGCATGGGCACACTCATGGGAGTGTACCTGCCCTGCCTGCAGAATATCTTTGGGGTCATCCTCTTC CTGCGGCTGACCTGGATGGTGGGCACAGCTGGTGTGCTGCAGGCTCTCCTCATTGTCCTCATCTGCTGCT GCTGTACCCTGCTGACAGCCATCTCCATGAGCGCCATTGCCACCAACGGCGTGGTTCCAGCTGGTGGCTC GTACTTCATGATTTCCCGCTCTTTGGGACCAGAATTCGGAGGTGCTGTGGGCCTGTGCTTCTACCTGGGG ACGACATTTGCAGCAGCCATGTATATCCTAGGGGCCATTGAGATCTTGCTGACCTACATTGCTCCGCCAG CTGCCATCTTTTACCCATCGGGCACCCATGACATGTCAAGCGCCACCTTGAATAACATGAGAGTGTATGG GACCATTTTCCTGACTCTGATGACCCTAGTGGTGTTTGTCGGTGTCAAGTACGTGAACAAGTTTGCCTCG CTCTTCCTGGCCTGCGTGATCATCTCCATCCTTTCCATTTACGCGGGAGGCATCAAGTCCATTTTTGACC CTCCCGTGTTTCCGGTGTGTATGCTGGGCAATAGGACCCTGTCTCGGGACCAGTTTGACATCTGTGCCAA GACAGTCGTGGTGGACAATGAGACAGTGGCCACCCGGCTGTGGACTTTCTTCTGCCACAGCCCCAACCTT ACTGCTGACTCCTGTGACCCCTACTTCCTGCTCAACAATGTGACAGAGATTCCTGGCATACCTGGGGCAG CTGCTGGTGTGCTCCAGGAGAACCTCTGGAGCGCTTACCTGGAGAAGGGGGAGGTCGTGGAGAAGCATGG GCTGCCGTCCACAGACACCCTCGGCCTAAAGGAGAGCCTGTCCCTGTATGTGGTGGCTGACATCGCCACG TCCTTCACCGTGCTGGTTGGCATCTTTTTCCCTTCTGTGACAGGCATCATGGCTGGTTCAAACCGCTCCG GGGATCTCCGTGACGCCCAGAAGTCTATCCCAGTGGGGACCATTCTGGCCATTGTTACCACTTCGCTGGT GTACTTCAGCAGTGTGATCCTCTTCGGTGCCTGCATCGAGGGTGTGGTACTCCGGGACAAGTACGGTGAT GGCGTCAGCAGGAACCTGGTGGTGGGCACCCTGGCCTGGCCTTCGCCTTGGGTCATCGTGGTTGGCTCCT TCTTCTCAACATGTGGGGCTGGTCTCCAGAGCCTCACTGGGGCGCCACGTTTACTGCAAGCCATTGCCAA GGATAACATCATCCCCTTCCTCCGGGTGTTTGGCCATGGGAAAGCAAACGGTGAACCGACGTGGGCCCTC CTCCTGACAGCGCTCATCGCTGAGCTGGGCATCCTGATTGCTTCCCTTGACATGGTGGCCCCCATTCTGT CCATGTTCTTTCTGATGTGTTACCTCTTTGTAAACTTGGCCTGTGCCGTGCAGACACTTCTGAGGACCCC CAACTGGCGGCCCCGGTTCAAGTACTATCACTGGACATTGTCTTTCCTAGGCATGAGTCTCTGCCTGGCT CTTATGTTTGTCTCCTCCTGGTACTATGCCCTAGTGGCCATGCTCATCGCAGGCATGATCTACAAGTACA TCGAGTACCAAGGGGCCGAGAAGGAGTGGGGCGATGGGATCCGAGGCCTGTCCCTGAGTGCGGCACGCTA TGCACTGCTGAGGCTGGAGGAAGGGCCTCCTCACACCAAGAACTGGCGGCCTCAGCTCCTGGTGCTGCTT AAGTTAGACGAAGATCTTCATGTGAAGTACCCCCGGCTCCTCACCTTCGCCTCCCAGCTGAAGGCCGGGA AGGGCCTGACAATCGTTGGCTCTGTCATCCAGGGCAGCTTTCTGGAGAGCTATGGTGAAGCCCAGGCCGC TGAGCAGACCATCAAGAACATGATGGACATTGAGAAAGTCAAAGGCTTCTGCCAGGTAGTGGTGGCCAGC AAGGTTCGAGAGGGACTGGCCCACCTCATCCAGTCTTGCGGCCTGGGCGGCATGAGACACAACTCCGTGG TGCTGGGCTGGCCCTATGGCTGGCGACAGAGTGAGGACCCACGTGCCTGGAAGACCTTTATTGACACTGT GCGCTGCACCACAGCTGCCCACCTGGCCCTGCTGGTGCCAAAGAACATAGCTTTCTACCCCAGCAACCAC GAGCGCTACCTGGATGGCCATATCGACGTGTGGTGGATCGTGCATGATGGAGGCATGCTTATGCTGCTGC CCTTTCTGCTGCGCCAGCATAAGGTTTGGAAGAAGTGCCGGATGCGCATTTTCACCGTGGCCCAGATGGA TGACAACAGCATCCAGATGAAGAAGGATCTGGCCATCTTCCTGTATCACCTCCGCCTGGAAGCTGAAGTG GAGGTGGTAGAGATGCACAACAGTGACATCTCTGCCTATACCTATGAGCGGACACTGATGATGGAGCAGC GGTCTCAGATGCTGCGGCAGATGAGGTTGACCAAAACTGAGCGGGATCGAGAGGCCCAGCTGGTAAAGGA CAGGCACTCGGCTCTTAGGCTGGAGAGCCTCTACTCCGACGAGGAGGAAGAGTCTGTGGCAGGGGCCGAC AAGATCCAGATGACGTGGACCAGAGACAAGTACATGGCTGAGCCCTGGGACCCCAGCCACGCCCCTGACA ACTTCCGGGAGCTGGTGCACATTAAGCCGGACCAGTCCAACGTGCGGCGCATGCACACTGCTGTGAAGCT CAACGAGGTCATTGTCACGCGCTCCCACGATGCCCGTCTGGTTTTGCTGAACATGCCTGGCCCCCCTAAG AACAGCGAGGGCGACGAGAACTACATGGAATTCCTTGAAGTCCTAACCGAGGGCCTTGAACGGGTGTTGT TGGTGCGTGGCGGCGGCCGGGAAGTCATCACCATCTATTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231563 representing NM_001253804 Red=Cloning site Green=Tags(s) MPHFTVVPVDGPRRGDYDNLEGLSWVDYGERAEREDSDGEGQGNHRENSPFLCPLDASRGNDYYDRNLAL FEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILF LRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLG TTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFAS LFLACVIISILSIYAGGIKSIFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVATRLWTFFCHSPNL TADSCDPYFLLNNVTEIPGIPGAAAGVLQENLWSAYLEKGEVVEKHGLPSTDTLGLKESLSLYVVADIAT SFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGD GVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWAL LLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLA LMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLL KLDEDLHVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVAS KVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNIAFYPSNH ERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEV EVVEMHNSDISAYTYERTLMMEQRSQMLRQMRLTKTERDREAQLVKDRHSALRLESLYSDEEEESVAGAD KIQMTWTRDKYMAEPWDPSHAPDNFRELVHIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPK NSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001253804
ORF Size	3261 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253804.1, NP_001240733.1
RefSeq Size	3832 bp
RefSeq ORF	3264 bp
Locus ID	20498
MW	121.3 kDa
Gene Summary	Mediates electroneutral potassium-chloride cotransport when activated by cell swelling. May contribute to cell volume homeostasis in single cells. May be involved in the regulation of basolateral Cl(-) exit in NaCl absorbing epithelia.[UniProtKB/Swiss-Prot Function]

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