Clasp2 (NM_001286601) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231567 representing NM_001286601 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCTCTGGATGTGCAGCAATCAGATTTATTATTCGGCATACCCATGTACCTAGACTTATTCCTT TAATAACAAGCAACTGCACATCAAAGTCAGTTCCTGTAAGGAGACGTTCATTTGAATTTTTAGATTTGCT GCTGCAAGAATGGCAGACTCATTCACTGGAAAGACATGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGC ATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCAAGGAAGACATACATGGGCCTTAGGAACCACTTTC CTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGCCATCATATCAGAAAAGTCTTCAAACTTACTTAAA GAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGACAGGTCCTCATCCAGCTCACAAGAAAGTCTCAAT CGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCTTCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCA GCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTTCTCGAAGTGACATTGATGTGAATGCGGCAGCTGG TGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCGAAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCA GGCTCCTATGCATCACTAGAGGATACTTCTGACAAGATGGATGGAACAGCATCTGATGATGGTCGGGTGA GAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAAATGCCAAGACCGACTCTAGAGGGAGAAGCCGGAC AAAAATGGTGTCTCAGTCCCAGCCTGGCAGCCGATCTGGGTCTCCAGGAAGAGTTCTAACCACAACAGCC CTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTGGTCAATTCAGCTTCAGCACAGAAGAGAAGCAAGA TCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCCCATCTCGGCTCTCAGTGGCCCGGAGCAGCCGTAT TCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGAAGCCAGCAGAGAGAGCAGCAGGGACACGAGTCCG GTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGGATCAGCCAGTCCAGCCGGTTGTCGTCCTCTGTCA GTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGGAGGAGGCAGTAGCTGATGCCCTGCTCTTAGGAGA CATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTATGAATCATATGGAATGCACTCAGATGATGATGCC AACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCCTATAGCTCTCGAAATGGTAGTATTCCTACCTACA TGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACAGATGTGCTAGCTCCAATTGGTCAGAGAGGAAAGA AGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCAGAGAACGCTAAGTCGAGTTGAACTGAAAAGATTA TGTGAAATTTTCACAAGAATGTTTGCAGATCCTCATGGCAAGAGAGTGTTCAGCATGTTCTTGGAGACTC TAGTAGATTTCATACAAGTCCACAAAGATGATCTTCAAGATTGGTTGTTTGTTCTGCTGACACAGCTGCT GAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCAGGCAAAAGTTCAGAAAGCCCTTGATATTACAAGA GAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTAATGAGATTTACAGTTGACCAGACCCAAACGCCAA GCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAGAAACTCTGGCAAAGCAGATGGACCCAAGAGATTT TACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCGGGTCATCACTTGGACGACAGAGCCCAAAAGCTCT GATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCTTTATTTGAACTCAATACCCCAGAGTTTACAATGT TACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTGCTACTAAACTTCTTCACAATCACCTCCGGAACAC TGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTTGACGAGACCAACACCTCGGTCACCAGCCAACTGG TCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAGAATACGTTATCTCCAAGTGCATTTGATTACGATA CAGAGAACATGAATTCTGAAGACATTTATAGCTCCCTTAGAGGCGTCACTGAGGCAATCCAGAATTTCAG CTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAGGAGGGACCCTAAAAAGGAGGATGGTGACACAATA TGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGAGGTGATGCTGCTGACGGCAGCCAGCCAGCTCTGG ATAATAAAGCATCGTTGCTCCACTCAATGCCACTCCACTCCTCTCCACGCTCCCGTGACTATAACCCATA TAACTACTCAGATAGCATCAGTCCTTTCAACAAGTCTGCCCTCAAGGAAGCCATGTTTGATGATGACGCC GACCAATTTCCTGATGATCTTTCCTTAGACCATTCTGACCTAGTTGCAGAGTTGTTGAAGGAGCTGTCTA ATCATAATGAACGTATAGAAGAAAGAAAAATTGCCCTGTATGAACTCATGAAGCTAACCCAGGAAGAATC TTTCAGTGTTTGGGATGAACACTTCAAAACAATATTATTGTTATTGCTTGAGACCCTTGGGGATAAAGAG CCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAAGAAATCTTAAGGCATCAACCAGCAAGATTCAAAA ACTATGCAGAACTAACTGTCATGAAAACACTGGAAGCACATAAAGATCCTCACAAAGAGGTGGTGAGATC TGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCATTAGTCCAGAGCAGTGCATCAAAGTGCTTTGTCCC ATCATACAAACCGCTGACTACCCTATTAATCTGGCTGCAATCAAAATGCAAACAAAAGTGATAGAGAGAG TATCCAAGGAGACCCTTAACATGCTCTTACCAGAGATCATGCCGGGTCTAATACAGGGTTATGATAATTC AGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCTGGTGGCTGTCCATGCAGTGATTGGTGATGAACTA AAGCCACATCTCAGTCAACTCACTGGTAGTAAAATGAAGCTGCTGAACCTTTACATCAAGCGTGCACAGA CGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTTCTGGACAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231567 representing NM_001286601 Red=Cloning site Green=Tags(s) MATSGCAAIRFIIRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKG IHDADAEARVEARKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLN RPFSSKWSTANPSTVAGRVSVGGSKANPLPGSLQRSRSDIDVNAAAGAKAHHAAGQAVRSGRLGAGALNP GSYASLEDTSDKMDGTASDDGRVRAKLSTPLVAVGNAKTDSRGRSRTKMVSQSQPGSRSGSPGRVLTTTA LSTVSSGAQRVLVNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSP VRSFQPLGPGYGISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYESYGMHSDDDA NSDASSACSERSYSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRL CEIFTRMFADPHGKRVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDITR ESFPNDLQFNILMRFTVDQTQTPSLKVKVAILKYIETLAKQMDPRDFTNSSETRLAVSRVITWTTEPKSS DVRKAAQSVLISLFELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANW SSPLTSPTNTSQNTLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMSEPVRRDPKKEDGDTI CSGPGMSDPRAGGDAADGSQPALDNKASLLHSMPLHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDA DQFPDDLSLDHSDLVAELLKELSNHNERIEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKE PTIRALALKVLKEILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCP IIQTADYPINLAAIKMQTKVIERVSKETLNMLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDEL KPHLSQLTGSKMKLLNLYIKRAQTGSAGADPTADVSGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286601
ORF Size	3267 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286601.1, NP_001273530.1
RefSeq Size	5644 bp
RefSeq ORF	3270 bp
Locus ID	76499
UniProt ID	Q8BRT1
MW	119.9 kDa
Gene Summary	Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function]

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