Pan2 (NM_001252327) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1200014O24Rik; AI047843; AW742773; mKIAA0710; Usp52
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231653 representing NM_001252327 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTGAGGGTCTGGATCCTGGACTGGCCGAATTTTCCCCAGCTATGCATTCTACCCTGGACCCAG TCCTGGATGCCCACCTGAATCCAAGTTTGCTACAGAATGTGGAGCTAGACCCAGAGGGAGTGGCCTTGGA GGCTCTTCCTGTCCAGGAGTCAGTGCACATAATGGAAGGTGTCTACTCTGAGTTGCACAGCGTGGTGGCT GAAGTGGGAGTGCCTGTGTCTGTCTCCCACTTTGATTTACATGAGGAGATGCTGTGGGTGGGGAGCCATG GGGTCAATGGTGGTGACGACATTCGGCAAATCCAGAGCCTGGAGAACGGTATCCTCTTCCTCACCAAGAA CAACCTCAAGTACATGGCCCGTGGAGGGCTCATTATATTTGACTATTTGCTGGATGAAAACGAGGATATG CACAGCGTCCTCCTGACAGACAACAGCACTCTGCTCGTTGGGGGGCTGCAGAACCACGTACTGGAGATTG ACCTGAACACTGTCCAGGAGACTCAGAAGTATGCAGTCGAGACACCCGGAGTCACCATCATGAGACAGAC AAATCGTTTCTTCTTCTGTGGCCACACATCTGGCAAGGTTTCCCTGCGAGACCTCCGTAGTTTTAAAGTG GAGCATGAATTTGATGCCTTCTCAGGGAGTCTGTCAGATTTTGATGTTCATGGCAACCTGCTGGCTGCCT GCGGCTTCTCCAGTCGCCTTACCGGCCTGGCCTGTGACCGTTTCCTCAAAGTGTACGATCTGCGCATGAT GCGTGCCATCACACCTCTTCAAGTGCACGTGGATCCGGCCTTCTTACGATTCATCCCCACATACACTTCC CGCCTTGCTATCATCTCCCAGTCAGGTCAATGCCAGTTTTGTGAACCCACAGGCCTGGCCAACCCAGCCG ACATTTTCCATGTGAATCCCGTGGGACCTTTGCTAATGACGTTTGATGTGTCAGCCAGCAAGCAGGCCCT GGCCTTTGGGGATTCTGAGGGCTGCGTGCATCTCTGGACTGATTCCCCTGAGCCGTCCTTCAACCCCTAC TCCCGAGAGACTGAGTTTGCCCTGCCCTGTCTTGTGGACTCCTTGCCGCCTCTGGACTGGAGCCAGGATC TGCTGCCGCTTTCCCTCATTCCCGTCCCACTTACCACTGATGCACTGCTCTCTGACTGGCCTGCTGCCAA CTCTGCTCCCGCTCCCAGGCGAGCACCACCTGTGGATGCAGAAATTCTTCGAACCATGAAGAAAGTGGGC TTCATTGGCTATGCTCCCAACCCTCGCACCAGGCTGCGCAACCAGATTCCCTATCGACTAAAGGAGTCAG ACCATGAATTTGACAACTTCAGCCAAGTCACGGAGTCACCGACAGGGCGAGAAGAGGAGCCTCTCCACAC AGTTTCTAAGAAATACCGGAAGGTAACCATCAAATATTCCAAGCTAGGCCTGGAAGACTTTGACTTCAAA CACTACAATAAGACTCTGTTTGCTGGGTTAGAGCCTCACATCCCCAATGCCTACTGTAACTGCATGATCC AGGTGCTCTATTTCTTGGAGCCTGTTCGCTGTCTGATCCAGAACCACCTTTGCCAAAAGGAGTTCTGCCT AGCGTGCGAGCTCGGCTTCCTCTTCCACATGCTAGACCTCTCTCGTGGCGATCCTTGTCAGGGCAGTAAT TTCCTCCGAGCTTTCCGGACCATCCCTGAGGCCTCAGCTCTGGGTCTCATCCTTGCTGACTCAGATGAGG CTTCAGGCAAGGGCAGTCTGGCCCGGCTCATCCAGAGGTGGAACCGCTTCATCCTCACTCAGCTACATCA GGATATGCAGGAGCTGGAAGTGCCCCAGGCTTATCGAGGTGCTGGAGGCAGTTTCTGCTCATCAGGAGAC TCCATCATTGGGCAGCTGTTCAGTTGTGAGATGGAGAACTGCAGTCTTTGCCGCTGCGGCAGCGAGACTG TGCGGGCCTCGTCCACCCTACTCTTCACACTCTCCTACCCTGAGGATAAAACCGGGAAGAACTATGACTT TGCTCAGGTGCTGAAGCGAAGTATCTGCCTGGAGCAGAACACACAGGCCTGGTGTGACAACTGTGAGAAG TACCAGCCCACAATTCAGACCAGAAACATCCGACATCTGCCAGATATTCTTGTCATTAACTGTGAAGTGA ATAGCTCAAAAGAGGCTGATTTCTGGAGACTGCAAGCTGAGGTTGCCTTCAAAATAGCGGTGAAAAAGTA CGGCGGGGAAATGAAGAGCAAAGAGTTTGCTTTAGCTGATCGGAAGGAACTGAGGAGTCCAGAGGGCTTT CTGTGTTCCTCCATCGAGGAGCTGAAGAATGTCTGGCTTCCATTTTCCATCCGCATGAAGATGACCAAGA ACAAAGGACTGGATGTTTGCAACTGGGCTGACGAGCATGAGTGGGGTCCGGCCAGGGCAGAGGAGGAGCT TGGTGTCTATGTATATGACCTGATGGCTACTGTGGTACACATCCTGGACTCACGAACAGGCGGCAGCTTG GTGGCTCACATCAAAGTCGGTGAGACCTACCACCAGCGCAAGGAGGGCGTGACTCATCAGCAGTGGTATC TCTTCAATGACTTCCTTATTGAACCTATTGATAAGTATGAAGCCGTGCAGTTTGACATGAATTGGAAAGT ACCTGCTATCCTGTATTATGTCAAAAGGAATCTCAATTCCAGATACAATCTGAATATCAAGAACCCCATT GAGGCCAGTGTGCTGCTGGCAGAAGCCTCGCTGGCACGGAAGCAACGGAAAACACACACGACCTTTATTC CATTGATGCTGAATGAGATGCCACAGGTCGGGGACCTGGTGGGCCTGGATGCCGAGTTTGTCACTCTTAA TGAGGAAGAAGCAGAGTTACGCAGTGATGGTACCAAGTCCACCATTAAGCCAAGCCAGATGTCAGTAGCA AGGATCACCTGTGTTCGGGGCCAAGGGCCTAACGAGGGCATCCCCTTCATTGATGACTACATCTCCACGC AGGAGCAGGTAGTAGATTACTTGACTCAGTACTCGGGGATAAAGCCAGGAGACCTTGATGCCAAAATCTC CTCAAAGCACCTCACAACCCTCAAGTCTACCTACTTAAAGCTTCGCTTTCTCATTGATATTGGAGTCAAG TTTGTGGGTCATGGTCTGCAGAAGGACTTCCGGGTCATCAACCTCATGGTGCCCAAGGACCAAGTTCTTG ACACAGTCTACCTGTTCCACATGCCCCGAAAACGAATGATTTCCCTACGATTCCTGGCCTGGTATTTTCT AGACCTGAAGATTCAAGGTGAGACCCATGACAGCATTGAGGATGCCCGCACAGCCCTTCAACTCTACCGG AAGTATCTGGAGCTGAGCAAGAACGGCACCGAGCCCGAGTCCTTCCACAAGGTGCTCAAGGGTCTCTATG AGAAGGGCCGAAAGATGGACTGGAAGGTGCCTGAGCCTGAAAGCCAGACAAGTCCCAAGAATGCAGCTGT CTTCTCAGTGCTGGCACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231653 representing NM_001252327 Red=Cloning site Green=Tags(s) MNFEGLDPGLAEFSPAMHSTLDPVLDAHLNPSLLQNVELDPEGVALEALPVQESVHIMEGVYSELHSVVA EVGVPVSVSHFDLHEEMLWVGSHGVNGGDDIRQIQSLENGILFLTKNNLKYMARGGLIIFDYLLDENEDM HSVLLTDNSTLLVGGLQNHVLEIDLNTVQETQKYAVETPGVTIMRQTNRFFFCGHTSGKVSLRDLRSFKV EHEFDAFSGSLSDFDVHGNLLAACGFSSRLTGLACDRFLKVYDLRMMRAITPLQVHVDPAFLRFIPTYTS RLAIISQSGQCQFCEPTGLANPADIFHVNPVGPLLMTFDVSASKQALAFGDSEGCVHLWTDSPEPSFNPY SRETEFALPCLVDSLPPLDWSQDLLPLSLIPVPLTTDALLSDWPAANSAPAPRRAPPVDAEILRTMKKVG FIGYAPNPRTRLRNQIPYRLKESDHEFDNFSQVTESPTGREEEPLHTVSKKYRKVTIKYSKLGLEDFDFK HYNKTLFAGLEPHIPNAYCNCMIQVLYFLEPVRCLIQNHLCQKEFCLACELGFLFHMLDLSRGDPCQGSN FLRAFRTIPEASALGLILADSDEASGKGSLARLIQRWNRFILTQLHQDMQELEVPQAYRGAGGSFCSSGD SIIGQLFSCEMENCSLCRCGSETVRASSTLLFTLSYPEDKTGKNYDFAQVLKRSICLEQNTQAWCDNCEK YQPTIQTRNIRHLPDILVINCEVNSSKEADFWRLQAEVAFKIAVKKYGGEMKSKEFALADRKELRSPEGF LCSSIEELKNVWLPFSIRMKMTKNKGLDVCNWADEHEWGPARAEEELGVYVYDLMATVVHILDSRTGGSL VAHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKYEAVQFDMNWKVPAILYYVKRNLNSRYNLNIKNPI EASVLLAEASLARKQRKTHTTFIPLMLNEMPQVGDLVGLDAEFVTLNEEEAELRSDGTKSTIKPSQMSVA RITCVRGQGPNEGIPFIDDYISTQEQVVDYLTQYSGIKPGDLDAKISSKHLTTLKSTYLKLRFLIDIGVK FVGHGLQKDFRVINLMVPKDQVLDTVYLFHMPRKRMISLRFLAWYFLDLKIQGETHDSIEDARTALQLYR KYLELSKNGTEPESFHKVLKGLYEKGRKMDWKVPEPESQTSPKNAAVFSVLAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252327
ORF Size	3519 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252327.1, NP_001239256.1
RefSeq Size	4369 bp
RefSeq ORF	3522 bp
Locus ID	103135
UniProt ID	Q8BGF7
MW	132.9 kDa
Gene Summary	Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity).[UniProtKB/Swiss-Prot Function]

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