Cadps2 (NM_001252106) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A230044C21Rik; Caps-2; Caps2; Cpd2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231677 representing NM_001252106 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGACCCGTCTTCCAGCGAGGAGGAGTCGGACGAGGGGCTGGAGGAGGAGAGCCGCGAGGTGTTGG TGGCGCCGGGCGTCTCCCAGCGAGCGCCGCCGGCCGCGGCTCGGGAAGGGCGGCGGGACGCGCCGGGACG CTCCGGTGGTGGCAGCGGCGGCGGAGCGGCCAGACCCGTGAGCCCGAGCCCCTCGGTACTCAGCGAGGGG CGAAATGAGCCCGAGCTGCAGCTGGACGAGGAGCAGGAGCGGCGCATCCGCCTGCAGCTCTACGTCTTCG TGGTGAGGTGCATCGCGTACCCATTCAACGCCAAGCAGCCCACCGACATGGCCCGGAGGCAGCAGAAGCT TAACAAACAACAATTGCAGTTACTGAAAGAACGGTTCCAGGCCTTTCTCAATGGAGAAACTCAGATAGTA GCAGACGAAGCCTTCTGCAATGCCGTCCGGAGTTACTATGAGGTGTTTCTAAAGAGTGACCGTGTGGCCA GAATGGTTCAAAGTGGAGGATGCTCTGCTAATGATTTCAGAGAGGTGTTTAAGAAGAACATAGAAAAACG TGTCCGTAGCCTGCCAGAGATAGATGGCCTGAGCAAAGAGACAGTGCTGAGCTCATGGATAGCCAAATAT GACGCCATTTACAGGGGAGAAGAAGATTTGTGCAAACAGCCGAACAGGATGACCCTCAGTGCCGTGTCTG AGCTTATTCTGAGTAAGGAGCAGCTCTATGAAATGTTCCAGCAGATTCTGGGCATTAAGAAGCTGGAACA CCAGCTACTTTATAACGCATGCCAGCTGGATAACGCTGATGAACAAGCAGCCCAGATCAGAAGGGAACTT GATGGCCGCCTGCAGTTGGCAGAGAAGATGGCAAAGGAGAGAAGATTCCCCAGATTCATCTCGAAAGAAA TGGAGAGTATGTACATTGAAGAGCTGCGAGCCTCCGTGAACCTGCTAATGGCCAATTTAGAAAGTCTTCC AGTTTCAAAAGGTGGCCCGGAATTTAAATTACAAAAATTAAAGCGTTCGCAGAACTCTGCGTTTCTGGAC CTGGGAGATGAGAACGAGATCCAGCTGTCCAAGTCGGATGTGGTGTTGTCGTTCACGTTAGAGATTGTCA TCATGGAAGTGCAAGGACTGAAATCTGTGGCTCCCAATCGAATCGTTTACTGCACAATGGAGGTGGAAGG AGGAGAAAAACTCCAGACAGACCAGGCTGAAGCATCAAGGCCACAATGGGGGACCCAAGGAGATTTCAAC ACTACCCACCCTCGGCCTGTCGTCAAAGTGAAGCTCTTCACAGAAAGCACGGGGGTCCTGGCCCTGGAAG ACAAGGAACTGGGCAGGGTGGTGTTATACCCAACTTCTAATAGCTCCAAGTCAGCAGAGTTACACCGAAT GACAGTACCCAAGAACAGTCAGGACTCGGACCTAAAGATCAAATTGGCAGTGCGCATGGATAAACCAGCA CACATGAAGCATAGTGGGTACCTGTATGCCCTTGGACAGAAGGTTTGGAAAAGATGGAAAAAGCGTTACT TTGTTCTCGTTCAGGTTAGCCAGTACACCTTTGCTATGTGCAGCTATAGAGAAAAAAAGTCGGAACCACA GGAATTAATGCAACTGGAAGGATACACAGTGGATTACACAGACCCTCACCCAGATGCAGACCGTTTTCAG AAACACGGGATGGATGAGTTTATTTCTGCGAGTCCTTGCAAGCTTGACCATGCCTTCCTCTTCAGAATTC TCCAGAGACAGACTTTGGATCACAGACTGAATGATTCGTATTCTTGTTTGGGGTGGTTTAGCCCTGGCCA AGTCTTTGTGTTAGATGAGTACTGTGCCCGCTACGGAGTGAGAGGCTGTCACAGGCATCTCTGCTACCTT ACAGAACTGATGGAACATTCAGAAAACGGTGCTGTCATTGACCCCACCCTGCTCCATTACAGCTTTGCAT TCTGTGCCTCTCACGTGCACGGCAACAGGCCTGATGGGATTGGAACGGTTTCAGTGGAAGAGAAAGAAAG ATTTGAGGAGATAAAAGACCGACTTTCTTCGCTTTTAGAAAACCAGATCAGCCACTTCAGATACTGCTTT CCCTTCGGACGACCTGAGGGTGCCCTAAAAGCTACGCTCTCCTTACTTGAAAGGGTTTTAATGAAAGACA TTGCCACTCCTATCCCTGCGGAGGAGGTGAAGAAAGTGGTCAGAAAATGTCTGGAGAAAGCTGCCTTGAT CAATTACACTAGGCTCACAGAATATGCCAAAATAGAAGGGCCCGCAGAAAAGGAAACAGAGACCATGAAC CAGGCAACTCCTGCCAGGAAGCTGGAAGAGGTTCTTCATCTTGCAGAGCTCTGCATAGAAGTCCTACAGC AAAATGAGGAGCATCATGCTGAGGGAAGAGAGGCATTTGCCTGGTGGCCTGACTTGTTGGCCGAGCATGC AGAGAAGTTTTGGGCTTTATTCACAGTAGACATGGATACTGCGCTGGAGGCCCAACCTCAAGACTCCTGG GATAGCTTTCCCCTTTTCCAGCTGCTTAATAATTTCCTCAGAAATGACACACTTTTGTGTAATGGAAAAT TCCACAAGCACTTGCAAGAAATCTTTGTGCCCTTGGTTGTCCGCTACGTTGACCTGATGGAGTCTGCCAT CGCCCAGTCCATTCACAGAGGTTTTGAGCAGGAGACATGGCAGCCTGTCAACAATGGCTCAACAACTTCC GAGGATCTGTTCTGGAAGCTCGATGCGCTGCAAATGTTCGTCTTTGATCTCCATTGGCCAGAACAAGAGT TTGCCCACCACTTAGAGCAAAGACTTAAACTAATGGCCAGTGATATGATAGAGGCGTGTGTCAAAAGAAC AAGAACTGCGTTCGAACTCAAGCTACAAAAGGCAAACAAAACAACTGACTTGCGCATCCCAGCTTCCGTG TGCACAATGTTTAATGTATTAGTTGATGCTAAAAAGCAAAGCACCAAGCTGTGTGCCCTGGATGGAGGAC AAGAGTTTAGGAATCAGTGGCAACAGTACCATTCAAAAATAGATGATTTGATTGACAACACCGTGAAAGA AATCATTGCACTGCTGGTTTCAAAGTTTGTTTCAGTGTTGGAAGGGGTGCTTTCGAAGTTGTCGAGGTAT GACGAAGGCACTTTCTTCTCATCCATCCTGTCCTTCACTGTGAAAGCAGCTGCAAAATATGTGGATGTCC CTAAACCAGGAATGGATCTGGCAGACACCTACATTATGTTTGTCCGGCAAAACCAGGATATTCTTCGAGA AAAAGTCAATGAAGAGATGTACATAGAAAAGTTGTTTGATCAATGGTACAGCAATTCCATGAAAGTCATT TGTGTGTGGCTGGCTGACAGACTAGACCTCCAGCTTCATATTTACCAACTGAAGACGCTCATCAAGATTG TGAAGAAAACCTATAGGGATTTCCGATTGCAGGGTGTGTTGGAGGGGACGCTGAACAGTAAGACATATGA TACTCTGCACAGACGTCTAACTGTAGAGGAGGCCACAGCCTCTGTCTCAGAAGGCGGAGGACTTCAGGGC ATTACCATGAAGGACAGTGATGAGGAGGAAGAAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231677 representing NM_001252106 Red=Cloning site Green=Tags(s) MLDPSSSEEESDEGLEEESREVLVAPGVSQRAPPAAAREGRRDAPGRSGGGSGGGAARPVSPSPSVLSEG RNEPELQLDEEQERRIRLQLYVFVVRCIAYPFNAKQPTDMARRQQKLNKQQLQLLKERFQAFLNGETQIV ADEAFCNAVRSYYEVFLKSDRVARMVQSGGCSANDFREVFKKNIEKRVRSLPEIDGLSKETVLSSWIAKY DAIYRGEEDLCKQPNRMTLSAVSELILSKEQLYEMFQQILGIKKLEHQLLYNACQLDNADEQAAQIRREL DGRLQLAEKMAKERRFPRFISKEMESMYIEELRASVNLLMANLESLPVSKGGPEFKLQKLKRSQNSAFLD LGDENEIQLSKSDVVLSFTLEIVIMEVQGLKSVAPNRIVYCTMEVEGGEKLQTDQAEASRPQWGTQGDFN TTHPRPVVKVKLFTESTGVLALEDKELGRVVLYPTSNSSKSAELHRMTVPKNSQDSDLKIKLAVRMDKPA HMKHSGYLYALGQKVWKRWKKRYFVLVQVSQYTFAMCSYREKKSEPQELMQLEGYTVDYTDPHPDADRFQ KHGMDEFISASPCKLDHAFLFRILQRQTLDHRLNDSYSCLGWFSPGQVFVLDEYCARYGVRGCHRHLCYL TELMEHSENGAVIDPTLLHYSFAFCASHVHGNRPDGIGTVSVEEKERFEEIKDRLSSLLENQISHFRYCF PFGRPEGALKATLSLLERVLMKDIATPIPAEEVKKVVRKCLEKAALINYTRLTEYAKIEGPAEKETETMN QATPARKLEEVLHLAELCIEVLQQNEEHHAEGREAFAWWPDLLAEHAEKFWALFTVDMDTALEAQPQDSW DSFPLFQLLNNFLRNDTLLCNGKFHKHLQEIFVPLVVRYVDLMESAIAQSIHRGFEQETWQPVNNGSTTS EDLFWKLDALQMFVFDLHWPEQEFAHHLEQRLKLMASDMIEACVKRTRTAFELKLQKANKTTDLRIPASV CTMFNVLVDAKKQSTKLCALDGGQEFRNQWQQYHSKIDDLIDNTVKEIIALLVSKFVSVLEGVLSKLSRY DEGTFFSSILSFTVKAAAKYVDVPKPGMDLADTYIMFVRQNQDILREKVNEEMYIEKLFDQWYSNSMKVI CVWLADRLDLQLHIYQLKTLIKIVKKTYRDFRLQGVLEGTLNSKTYDTLHRRLTVEEATASVSEGGGLQG ITMKDSDEEEEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252106
ORF Size	3606 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252106.1, NP_001239035.1
RefSeq Size	4663 bp
RefSeq ORF	3609 bp
Locus ID	320405
MW	138.1 kDa
Gene Summary	Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates neurotrophin release from granule cells leading to regulate cell differentiation and survival during cerebellar development. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles.[UniProtKB/Swiss-Prot Function]

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