Kcnt1 (NM_001302351) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	C030030G16Rik; s; Sl; Slack; slo2; Slo2.2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231697 representing NM_001302351 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCGGGCCAAGCTGCCGCGCTCGCCGTCCGAGGGCAAGGCGGGTCCGGGGGACACCCCAGCCGGCG CTGCAGCCCCTGAGGAGCCACACGGGCTCAGCCCGCTACTGCCGGCCCGCGGCGGGGGCTCCGTGGGCAG CGACGTGGGCCAGAGGGTCCAGGTGGAGTTCTATGTCAATGAGAACACCTTCAAAGAGCGGCTCAAGCTG TTCTTCATCAAAAACCAAAGATCCAGCCTGAGGATCCGGCTGTTCAACTTCTCCCTCAAGCTCCTCACCT GCCTGCTGTATATCGTCCGTGTCCTGCTTGACAACCCAGACCAGGGCATCGGATGCTGGGGCTGCACGAA GTATAACTACACTTTCAATGGCTCATCCTCTGAGTTCCACTGGGCTCCCATCCTGTGGGTGGAGAGGAAA ATGGCTCTGTGGGTGATCCAGGTCATTGTGGCCACAATAAGCTTCTTAGAGACCATGCTCATCATTTACC TCAGCTACAAAGGCAACATCTGGGAGCAGATATTCCATGTGTCTTTCGTCTTGGAGATGATCAACACACT GCCCTTCATCATCACGGTCTTCTGGCCACCTCTGCGGAACCTGTTCATCCCCGTGTTTCTCAACTGCTGG CTGGCCAAGCATGCGCTGGAGAACATGATTAATGACTTCCACCGTGCCATCCTACGCACACAGTCAGCCA TGTTCAACCAGGTGCTCATCCTGTTCTGCACCCTGCTGTGCCTGGTCTTCACAGGGACCTGTGGGATCCA GCACCTGGAGCGAGCAGGTGGCAACTTGAACCTGCTGACCTCCTTCTACTTCTGCATCGTGACTTTCTCA ACTGTGGGCTTCGGTGATGTGACGCCCAAGATCTGGCCATCCCAGCTCCTGGTGGTCATCCTGATCTGTG TCACCCTTGTGGTGCTCCCACTGCAATTTGAAGAGCTTGTCTACCTCTGGATGGAGCGTCAGAAGTCAGG GGGCAACTATAGCCGCCACCGAGCACGGACGGAGAAGCACGTAGTCCTGTGTGTGAGCTCCCTCAAGATT GATCTCCTCATGGACTTCCTGAATGAGTTCTATGCCCATCCCCGGCTCCAGGACTACTACGTGGTCATCC TGTGTCCCTCTGAAATGGACGTCCAGGTGCGCAGGGTGCTGCAGATTCCCCTGTGGTCCCAGCGGGTCAT CTACCTCCAGGGCTCTGCCCTCAAGGACCAGGATCTCATGCGAGCCAAGATGGACAACGGAGAGGCCTGC TTTATCCTCAGCAGCAGGAATGAGGTGGACCGCACAGCTGCGGATCACCAGACCATCCTTCGAGCCTGGG CTGTGAAGGACTTTGCCCCCAACTGTCCCCTCTATGTCCAGATCCTCAAGCCCGAAAACAAGTTTCACGT CAAATTTGCTGACCACGTGGTATGCGAGGAAGAGTGCAAGTACGCCATGCTGGCCCTGAACTGCATCTGC CCGGCCACCTCCACCCTCATCACCCTGCTGGTGCACACGTCCCGTGGCCAGGAAGGACAGGAGTCTCCCG AGCAGTGGCAGCGCACGTACGGGAGGTGCTCGGGCAACGAGGTGTACCACATTCGCATGGGTGACAGTAA ATTCTTCCGGGAGTATGAGGGCAAGAGCTTCACCTACGCAGCCTTCCACGCGCACAAGAAATATGGGGTG TGCCTCATCGGGCTGAAGCGTGAGGAGAACAAGAGTATCCTGCTGAACCCAGGACCACGGCACATCCTGG CTGCCTCCGACACCTGCTTCTATATCAATATTACCAAGGAGGAGAACTCAGCTTTCATCTTCAAACAGGA GGAGAAGCAGAAGAGACGGGGCCTTGCAGGGCAGGCACTATATGAAGGGCCCTCCCGGCTCCCAGTGCAT AGCATCATCGCCTCTATGGGGACAGTGGCCATGGACCTGCAGAACACAGATTGCCGGCCCTCCCAGGGTG GCAGTGGCGGGGACGGCACAAAGCTGACTCTGCCCACCGAGAACGGCTCTGGTAGTCGACGCCCCAGCAT CGCACCCGTTCTGGAGTTGGCAGACAGCTCAGCCCTGTTGCCCTGCGACCTGCTGAGTGACCAGTCAGAG GATGAGGTGACACCCTCGGACGACGAGGGGCTCTCTGTGGTTGAGTACGTGAAGGGCTATCCCCCCAACT CACCCTACATTGGCAGCTCCCCGACTTTATGCCACCTCCTGCCTGTGAAAGCCCCCTTCTGCTGCCTGCG GTTGGACAAGGGCTGCAAACACAACAGCTACGAGGATGCCAAGGCCTATGGGTTCAAGAACAAGCTGATT ATTGTCTCTGCTGAGACGGCAGGCAACGGGCTCTACAACTTCATCGTGCCTCTGCGCGCCTACTACCGGT CCCGCCGGGAGCTCAACCCTATCGTGTTGCTGCTTGACAACAAGCCTGACCACCACTTCCTGGAGGCCAT CTGTTGCTTCCCCATGGTCTACTACATGGAGGGATCCGTGGACAACCTGGACAGCTTACTGCAGTGTGGC ATCATCTATGCTGACAACCTGGTGGTGGTGGACAAGGAGAGCACTATGAGCGCTGAAGAGGACTACATGG CAGATGCCAAGACCATCGTCAATGTGCAAACCATGTTCCGGCTTTTCCCCAGTCTCAGCATCACCACGGA GCTCACACACCCTTCCAACATGCGGTTCATGCAGTTCCGTGCCAAGGACAGCTACTCTCTGGCTCTTTCC AAACTTGAAAAGCAAGAACGGGAGAACGGCTCCAACCTGGCCTTCATGTTCCGCCTGCCATTTGCTGCTG GTCGAGTATTTAGTATCAGCATGTTGGATACACTGCTCTACCAGTCCTTCGTGAAGGACTACATGATCAC CATCACCAGGCTGCTCTTGGGCCTTGATACTACACCAGGCTCCGGCTACCTCTGTGCAATGAAGGTAACC GAGGACGACCTGTGGATCCGCACTTACGGCCGCCTCTTCCAGAAACTCTGCTCCTCCAGCGCCGAGATCC CCATCGGCATCTACAGGACCGAGTGCCATGTCTTCTCGGAGCCCCATGACGTCAGAGCCCAGTCTCAGAT CTCGGTGAACATGGAGGACTGCGAGGATACTCGGGAGGCCAAGGGACCCTGGGGCACACGAGCTGCATCT GGCAGTGGCAGCACCCATGGCCGTCACGGGGGCAGTGCTGACCCAGTGGAGCACCCACTACTACGTCGCA AGAGCCTGCAGTGGGCCCGCAAGCTGAGTCGCAAGAGCACCAAGCAGGCAGGGAAGGCACCTGTGGCCAC AGACTGGATCACCCAGCAGCGGCTCAGCCTGTACCGACGCTCAGAGCGCCAGGAGCTCTCAGAGCTGGTC AAGAACCGAATGAAGCACCTGGGACTGCCCACCACTGGCTATGAGGACGTAGCAAATTTAACAGCCAGTG ATGTGATGAATCGGGTAAACCTGGGATATTTGCAAGATGAGATGAACGATCATCACCAGAACACCCTTTC CTATGTACTCATCAACCCCCCGCCAGACACAAGACTGGAACCCAACGACATTGTGTACCTCATCCGTTCC GACCCCCTGGCCCATGTGGCCAGCAGCTCCCAGAGTCGGAAAAGCAGCTGCAGCAACAAGCTCTCATCCT GTAATCCTGAGACCAGGGATGAGACCCAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231697 representing NM_001302351 Red=Cloning site Green=Tags(s) MARAKLPRSPSEGKAGPGDTPAGAAAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKL FFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDNPDQGIGCWGCTKYNYTFNGSSSEFHWAPILWVERK MALWVIQVIVATISFLETMLIIYLSYKGNIWEQIFHVSFVLEMINTLPFIITVFWPPLRNLFIPVFLNCW LAKHALENMINDFHRAILRTQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGGNLNLLTSFYFCIVTFS TVGFGDVTPKIWPSQLLVVILICVTLVVLPLQFEELVYLWMERQKSGGNYSRHRARTEKHVVLCVSSLKI DLLMDFLNEFYAHPRLQDYYVVILCPSEMDVQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEAC FILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEECKYAMLALNCIC PATSTLITLLVHTSRGQEGQESPEQWQRTYGRCSGNEVYHIRMGDSKFFREYEGKSFTYAAFHAHKKYGV CLIGLKREENKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRRGLAGQALYEGPSRLPVH SIIASMGTVAMDLQNTDCRPSQGGSGGDGTKLTLPTENGSGSRRPSIAPVLELADSSALLPCDLLSDQSE DEVTPSDDEGLSVVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLI IVSAETAGNGLYNFIVPLRAYYRSRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLQCG IIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALS KLEKQERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMITITRLLLGLDTTPGSGYLCAMKVT EDDLWIRTYGRLFQKLCSSSAEIPIGIYRTECHVFSEPHDVRAQSQISVNMEDCEDTREAKGPWGTRAAS GSGSTHGRHGGSADPVEHPLLRRKSLQWARKLSRKSTKQAGKAPVATDWITQQRLSLYRRSERQELSELV KNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHHQNTLSYVLINPPPDTRLEPNDIVYLIRS DPLAHVASSSQSRKSSCSNKLSSCNPETRDETQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302351
ORF Size	3672 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302351.1, NP_001289280.1
RefSeq Size	5945 bp
RefSeq ORF	3675 bp
Locus ID	227632
UniProt ID	Q6ZPR4
MW	138.6 kDa
Gene Summary	This gene encodes a member of the Slo potassium channel family that has shown to be activated by both sodium and chloride ions. This channel represents the largest potassium channel subunit yet identified. This channel may be important in development and pain signaling. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

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