Dctn1 (NM_001198867) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AL022633; DAP-150; DP-150; Glued; p150; p150-glued; p150
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231717 representing NM_001198867 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTACGGAGGCAAGCGCCCGGCCCCTGCGGGTTGGCTCCCGCGTGGAGGTGATTGGGAAGGGCCACC GAGGCACTGTGGCCTATGTTGGAGCCACACTCTTTGCCACTGGCAAATGGGTGGGCGTGATTCTGGATGA AGCAAAAGGCAAAAATGATGGCACTGTCCAGGGAAGGAAGTATTTCACATGTGATGAAGGCCACGGCATC TTTGTACGCCAGTCCCAGATCCAAGTATTTGAAGATGGAGCAGATACTACTTCCCCAGAGACTCCTGATT CTTCTGCTTCAAAGGTCCTCAAGAGAGAGGGAGCCGATGCAGCTGCAAAGACCAGCAAACTGCCTACTCG CCCAGCCAGCACTGGGGTGGCTGGGCCCAGTAGCTCCCTTGGCCCCTCTGGCTCAGCGTCAGCCGGGGAA CTAAGCAGCAGTGAGCCCAGCACCCCAGCTCAGACTCCGCTGGCAGCACCCATCATCCCCACACCGGCCC TCACCTCTCCTGGAGCAGCACCCCCACTTCCATCTCCCTCTAAGGAAGAGGAAGGGCTGAGGGCTCAGGT ACGGGACCTGGAGGAGAAGCTGGAGACCCTGCGCCTAAAACGCTCAGAAGACAAAGCAAAGCTGAAAGAG CTGGAGAAGCACAAGATCCAGCTGGAGCAGGTGCAGGAATGGAAGAGCAAAATGCAGGAGCAGCAGGCAG ACCTGCAGCGGCGCCTCAAGGAGGCTCGGAAGGAAGCCAAGGAGGCGCTAGAGGCAAAGGAACGCTACAT GGAGGAGATGGCCGACACAGCCGACGCTATCGAGATGGCCACTCTGGACAAGGAGATGGCTGAAGAGCGC GCTGAGTCTCTGCAGCAAGAGGTGGAGGCACTGAAGGAACGGGTAGACGAGCTCACCACAGACCTGGAGA TTCTCAAGGCTGAAATCGAAGAGAAAGGCTCTGATGGGGCCGCATCAAGCTACCAGCTCAAGCAGCTGGA GGAGCAGAATGCCCGCCTGAAGGATGCCCTGGTGAGGATGCGAGACCTCTCTTCCTCAGAGAAGCAGGAG CACGTGAAGCTGCAGAAACTCATGGAAAAGAAAAACCAGGAGCTGGAGGTCGTGCGGCAGCAGCGCGAGC GTCTTCAGGAGGAGCTGAGCCAGGCTGAGAGCACCATCGATGAGCTCAAAGAGCAGGTGGACGCCGCTCT GGGAGCCGAGGAGATGGTGGAGATGCTGACCGACCGGAACCTGAATCTAGAGGAGAAAGTGCGGGAGTTA CGGGAGACTGTGGGGGACTTGGAAGCCATGAACGAGATGAACGATGAGCTGCAGGAGAACGCACGGGAGA CGGAGCTGGAACTCCGAGAGCAGCTGGACATGGCGGGCGCCCGAGTGAGGGAAGCGCAGAAGCGAGTGGA AGCCGCCCAGGAGACAGTCGCCGACTACCAGCAGACCATCAAGAAGTACCGCCAGTTGACTGCCCACCTA CAGGATGTCAATCGGGAGCTGACAAACCAGCAGGAAGCGTCTGTAGAGAGGCAGCAGCAGCCGCCGCCAG AGACTTTTGATTTCAAAATCAAGTTTGCTGAGACCAAGGCTCATGCCAAGGCCATTGAGATGGAGTTGAG ACAGATGGAAGTTGCCCAGGCCAACCGGCACATGTCCCTGCTGACAGCCTTTATGCCTGACAGCTTCCTT CGGCCAGGTGGAGACCACGACTGTGTCCTGGTGCTGCTGCTCATGCCCCGACTCATTTGCAAGGCAGAGC TCATCCGGAAGCAGGCCCAGGAGAAGTTTGACCTGAGCGAGAACTGTTCGGAGCGGCCCGGGCTGCGGGG AGCTGCCGGGGAGCAGCTGAGCTTTGCTGCTGGACTGGTGTACTCGCTGAGTCTGCTGCAGGCCACGCTG CACCGCTATGAGCATGCCCTCTCTCAGTGCAGTGTGGACGTGTATAAGAAGGTCGGCAGCCTGTACCCCG AGATGAGCGCCCACGAGCGCTCCTTAGATTTCCTCATTGAGCTGCTGCACAAGGATCAGCTGGATGAGAC TGTCAACGTGGAGCCCCTCACCAAGGCCATCAAGTATTACCAGCATCTGTACAGCATCCACCTCGCTGAA CAACCCGAGGACTCCACCATGCAGCTGGCTGACCACATCAAGTTCACCCAGAGTGCCCTGGACTGCATGG GCGTGGAGGTGGGGCGGCTGCGTGCCTTCTTGCAGGGTGGGCAGGAGGCAACAGATATTGCCCTTCTTCT CCGAGACCTGGAAACATCATGTAGTGACACCCGTCAGTTCTGCAAGAAGATCCGAAGGCGGATGCCGGGG ACGGATGCTCCTGGGATCCCAGCAGCGCTGGCCTTTGGCTCACAGGTATCCGACACACTCCTGGACTGCA GGAAGCACTTGACGTGGGTGGTAGCTGTTCTGCAGGAGGTGGCAGCTGCAGCCGCCCAGCTTATTGCCCC CTTGGCAGAGAACGAGGGGCTGCCTGTGGCTGCACTGGAGGAGCTGGCCTTCAAAGCAAGCGAGCAGATC TACGGGAGCCCCTCCAGCAGCCCCTATGAGTGTCTACGCCAGTCATGCACCATCCTCATCAGCACGATGA ACAAGCTGGCCACAGCCATGCAAGAAGGCGAGTATGACGCAGAGCGACCCCCGAGCAAGCCTCCTCCGGT TGAACTTCGGGCTGCAGCCCTGCGTGCAGAGATCACAGATGCTGAAGGTCTGGGTTTGAAGCTTGAGGAT CGAGAGACAGTTATCAAGGAGTTAAAGAAGTCACTCAAGATTAAGGGAGAGGAGCTGAGTGAGGCCAACG TGCGGCTCAGCCTCCTGGAGAAGAAGTTGGACAGCGCTGCCAAGGATGCAGACGAGCGAATCGAGAAAGT TCAGACACGGCTGGACGAGACTCAGACCCTGCTGCGGAAGAAGGAGAAAGACTTTGAGGAGACAATGGAC GCACTCCAGGCTGACATCGACCAGCTGGAGGCAGAGAAGGCAGAGCTCAAGCAGCGCCTGAACAGCCAGT CCAAGCGCACAATCGAGGGGCTCCGGGGCCCCCCTCCGTCAGGCATCGCTACCCTGGTCTCTGGCATCGC TGGTGGGGGCGCTCCTGGGCAGGCTCCGGGCGCCTTGCCAGGCCCGGGGCTGGTGAAGGACTCCCCACTG CTGCTTCAGCAGATCTCTGCTATGAGGCTACACATCTCTCAGCTCCAGCATGAGAACAGCATCCTCAGAG GAGCCCAGATGAAGGCGTCCTTGGCAGCTCTGCCCCCTCTGCATGTTGCAAAGCTTTCCCTCCCACCCCA TGAGGGCCCCGGTGGTAACCTAGTGGCTGGGGCACTGTACCGCAAGACCAGCCAGCTCCTGGAGAAACTA AACCAGCTGAGTACCCACACCCACGTGGTGGATATCACTCGGAGCAGCCCAGCTGCCAAGAGCCCGTCAG CTCAGCTTATGGAACAAGTGGCTCAGCTCAAGTCCCTGAGTGACACCATTGAGAAGCTCAAGGATGAGGT CCTCAAGGAGACAGTGACTCAGCGCCCTGGAGCCACTGTGCCCACCGACTTTGCCACTTTCCCTTCATCT GCCTTCCTCAGGGCCAAGGAAGAGCAGCAAGATGACACAGTCTACATGGGCAAAGTGACCTTTTCATGCG CGGCAGGCCTAGGACAGCGACACCGCCTGGTGCTGACCCAGGAGCAGCTGCACCAGCTTCACAGTCGCCT CATCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231717 representing NM_001198867 Red=Cloning site Green=Tags(s) MSTEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGI FVRQSQIQVFEDGADTTSPETPDSSASKVLKREGADAAAKTSKLPTRPASTGVAGPSSSLGPSGSASAGE LSSSEPSTPAQTPLAAPIIPTPALTSPGAAPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRSEDKAKLKE LEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEER AESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQE HVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVREL RETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHL QDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFL RPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFDLSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATL HRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAE QPEDSTMQLADHIKFTQSALDCMGVEVGRLRAFLQGGQEATDIALLLRDLETSCSDTRQFCKKIRRRMPG TDAPGIPAALAFGSQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLPVAALEELAFKASEQI YGSPSSSPYECLRQSCTILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLED RETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLDETQTLLRKKEKDFEETMD ALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGGGAPGQAPGALPGPGLVKDSPL LLQQISAMRLHISQLQHENSILRGAQMKASLAALPPLHVAKLSLPPHEGPGGNLVAGALYRKTSQLLEKL NQLSTHTHVVDITRSSPAAKSPSAQLMEQVAQLKSLSDTIEKLKDEVLKETVTQRPGATVPTDFATFPSS AFLRAKEEQQDDTVYMGKVTFSCAAGLGQRHRLVLTQEQLHQLHSRLIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198867
ORF Size	3717 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198867.1, NP_001185796.1
RefSeq Size	4172 bp
RefSeq ORF	3720 bp
Locus ID	13191
MW	137.3 kDa
Gene Summary	Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitement to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.[UniProtKB/Swiss-Prot Function]

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