Ppfia2 (NM_001205341) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	5330438O12; B230207K17Rik; E130120L08Rik; mKIAA4112
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231733 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGTGAAGTGATGCCCACGATTAACGAGGACACCCCAATGAGCCAACGGGGTTCCCAAAGCAGTG GCTCCGACTCGGACTCCCATTTTGAGCAACTCATGGTCAATATGCTGGATGAAAGGGACCGCCTACTGGA CACACTCCGAGAGACCCAGGAGAGCCTCTCACTTGCCCAGCAAAGGCTCCAGGATGTCATCTATGACAGA GATTCACTCCAGAGACAGCTGAATTCAGCCCTACCTCAGGACATCGAATCCCTAACAGGAGGGCTGACTG GTTCTAAGGGGGCTGATCCACCGGAGTTTGCTGCACTGACTAAAGAGTTGAATGCTTGCCGTGAACAACT TCTAGAAAAGGAAGAAGAAATCTCAGAGCTGAAAGCCGAAAGAAACAACACAAGACTGTTACTGGAACAC TTGGAGTGCCTTGTGTCCCGACATGAACGGTCACTAAGAATGACGGTGGTCAAGCGGCAAGCCCAGTCTC CCTCTGGAGTGTCCAGTGAAGTGGAGGTTCTCAAGGCACTGAAATCTTTGTTTGAACACCACAAAGCCTT GGATGAAAAGGTAAGGGAGCGACTGAGGGTTTCTTTAGAAAGAGTCTCTGCACTGGAAGAGGAACTAGCT GCAGCTAATCAGGAGATTGTTGCCTTGCGTGAGCAAAATGTTCATATTCAAAGGAAAATGGTATCAAGTG AGGGGTCCACGGAGTCAGAGCATCTTGAAGGAATGGAAGCAGGCCAGAAAGTTCATGAAAAGCGTCTATC CAATGGCTCCATAGATTCGACAGATGACACCAGCCAAATCGTCGAGCTGCAAGAATTGCTTGAGAAGCAA AACTATGAAATGGCCCAAATGAAAGAACGCTTAACAGCTCTCTCTTCCCGGGTGGGAGAGGTGGAACAAG AAGCAGAGACAGCAAGAAAGGACCTCATTAAAACAGAAGAAATGAACACAAAATATCAAAGGGACATCCG AGAGGCCATGGCTCAGAAGGAAGATATGGAAGAAAGAATCACAACTCTGGAGAAGCGTTACCTGAGTGCT CAGAGAGAATCTACCTCCATTCATGATATGAATGATAAACTAGAAAATGAATTGGCGAACAAGGAAGCCA TCTTGCGGCAGATGGAAGAAAAAAACAGGCAATTGCAAGAGCGTCTTGAACTGGCTGAGCAAAAGTTACA GCAGACCATGAGAAAAGCTGAAACTTTACCTGAAGTAGAGGCTGAACTTGCTCAGAGAATTGCAGCCCTA ACAAAGGCTGAAGAGAGGCATGGAAATATTGAAGAACGTATGAGACACCTCGAGGGCCAACTTGAAGAGA AGAATCAAGAACTTCAAAGAGCTAGACAAAGGGAGAAAATGAATGAAGAGCATAATAAGAGATTATCGGA TACGGTGGACAGGCTTCTGACAGAATCCAATGAGCGCCTGCAACTACACCTGAAGGAAAGGATGGCTGCT TTGGAAGAGAAGAATGTTTTGATTCAAGAATCAGAAAACTTCAGAAAAAATCTTGAAGAGTCCTTACATG ATAAGGAAAGATTAGCAGAAGAAATCGAGAAGCTGAGGTCTGAACTTGATCAAATGAAAATGAGAACAGG TTCTCTAATTGAACCCACTATATCAAGAACTCATATAGACACCTCCACTGAGTTGCGGTATTCAGTTGGG TCCCTTGTAGACAGCCAGTCTGATTACAGAACCACTAAAGTAATACGAAGACCAAGGAGAGGCCGCATGG GTGTGCGAAGAGATGAACCAAAGGTGAAATCCCTTGGAGATCATGAGTGGAACAGAACTCAGCAAATTGG AGTACTAGGAAGCCACCCTTTCGAAAGTGACACTGAAATGTCTGATATTGATGATGATGACAGAGAAACA ATTTTTAGCTCAATGGATCTTCTCTCTCCAAGTGGCCATTCTGATGCCCAGACTCTAGCTATGATGCTTC AAGAACAATTGGATGCAATCAATAAAGAAATAAGACTAATTCAAGAAGAAAAGGAATCGACAGAATTGCG TGCTGAAGAAATTGAGAATAGAGTGGCTAGTGTAAGCTTAGAAGGGCTGAATTTGGCGAGGGTCCATCCA GGTACCTCCATCACTGCCTCTGTTACAGCTTCATCACTGGCCAGTTCATCTCCCCCAAGTGGACACTCAA CGCCAAAGCTTACACCAAGAAGCCCTGCCAGGGAAATGGACCGCATGGGAGTGATGACCCTGCCAAGTGA TCTAAGGAAACATCGGAGAAAGATTGCAGTGGTGGAAGAAGATGGACGGGAGGATAAAGCCACAATTAAA TGCGAGACTTCTCCTCCCCCAACACCCAGAGCCGTCAGAATGACTCACACCTTACCATCTTCCTACCACA ATGATGCCCGGAGCAGTTTATCTGCCTCTCTTGAGCCAGACAGTCTTGGGCTTGGCAGTGCCAATAGCAG CCAAGATTCTCTCCACAAAGCCCCCAAGAAGAAAGGAATCAAGTCTTCAATTGGGCGCTTATTTGGGAAA AAAGAGAAGGCTCGACTCGGGCAGCTTCGAGGCTTCATGGAGACAGAAGCTGCAGCACAGGAATCCCTGG GCTTAGGCAAACTTGGAACTCAAGCTGAAAAGGACAGAAGGCTGAAGAAAAAGCATGAACTTCTTGAAGA AGCTCGTAGAAAAGGATTACCTTTTGCCCAGTGGGATGGACCCACCGTGGTTGCGTGGCTGGAGCTCTGG CTGGGAATGCCAGCTTGGTACGTGGCAGCATGCAGAGCCAACGTGAAAAGTGGTGCCATCATGTCAGCGT TATCGGACACTGAAATCCAAAGGGAGATTGGAATCAGCAACCCTCTGCACCGCTTAAAGCTCAGGCTAGC TATCCAAGAGATGGTTTCTCTCACCAGCCCTTCAGCGCCTCCGACATCGCGTACTCCTTCAGGCAATGTG TGGGTGACCCATGAAGAAATGGAAAATCTGACAGCTCCAGCAAAAACGAAAGAATCTGAGGAAGGAAGCT GGGCCCAGTGTCCGGTTTTTCTACAGACCCTGGCTTATGGAGATATGAACCACGAGTGGATTGGAAATGA ATGGCTTCCCAGCCTGGGGTTACCTCAATACAGAAGTTACTTTATGGAATGCTTGGTTGATGCAAGAATG TTAGACCACCTCACAAAAAAGGATCTTCGTGTCCATTTAAAAATGGTGGATAGTTTCCATCGAACAAGTT TACAGTATGGAATTATGTGCTTGAAAAGATTGAATTATGACAGAAAAGAACTAGAGAGAAGACGAGAAGC AAGTCAACATGAAATAAAAGATGTTTTAGTGTGGAGCAATGATCGAGTTATTCGCTGGATACAAGCAATT GGACTTCGGGAATATGCAAACAACATTCTTGAGAGTGGCGTGCATGGCTCACTTATAGCCCTGGATGAGA ACTTTGACTACAGCAGCTTGGCTCTATTGTTGCAGATTCCAACACAGAACACCCAGGCAAGGCAGATTCT TGAACGTGAGTACAACAACCTCTTGGCCCTGGGAACTGAGCGGAGACTGGATGAAAGTGACGACAAGAAT TTCAGACGGGGATCAACCTGGCGAAGGCAGTTCCCTCCTCGTGAAGTTCATGGAATCAGCATGATGCCTG GGTCCTCAGAAACATTACCAGCTGGATTCAGGTTGACCACAACGTCTGGGCAGTCCAGGAAAATGACGAC AGACGTTGCTTCATCAAGACTGCAGAGGTTAGACAACTCCACTGTTCGCACATACTCATGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231733 protein sequence Red=Cloning site Green=Tags(s) MMCEVMPTINEDTPMSQRGSQSSGSDSDSHFEQLMVNMLDERDRLLDTLRETQESLSLAQQRLQDVIYDR DSLQRQLNSALPQDIESLTGGLTGSKGADPPEFAALTKELNACREQLLEKEEEISELKAERNNTRLLLEH LECLVSRHERSLRMTVVKRQAQSPSGVSSEVEVLKALKSLFEHHKALDEKVRERLRVSLERVSALEEELA AANQEIVALREQNVHIQRKMVSSEGSTESEHLEGMEAGQKVHEKRLSNGSIDSTDDTSQIVELQELLEKQ NYEMAQMKERLTALSSRVGEVEQEAETARKDLIKTEEMNTKYQRDIREAMAQKEDMEERITTLEKRYLSA QRESTSIHDMNDKLENELANKEAILRQMEEKNRQLQERLELAEQKLQQTMRKAETLPEVEAELAQRIAAL TKAEERHGNIEERMRHLEGQLEEKNQELQRARQREKMNEEHNKRLSDTVDRLLTESNERLQLHLKERMAA LEEKNVLIQESENFRKNLEESLHDKERLAEEIEKLRSELDQMKMRTGSLIEPTISRTHIDTSTELRYSVG SLVDSQSDYRTTKVIRRPRRGRMGVRRDEPKVKSLGDHEWNRTQQIGVLGSHPFESDTEMSDIDDDDRET IFSSMDLLSPSGHSDAQTLAMMLQEQLDAINKEIRLIQEEKESTELRAEEIENRVASVSLEGLNLARVHP GTSITASVTASSLASSSPPSGHSTPKLTPRSPAREMDRMGVMTLPSDLRKHRRKIAVVEEDGREDKATIK CETSPPPTPRAVRMTHTLPSSYHNDARSSLSASLEPDSLGLGSANSSQDSLHKAPKKKGIKSSIGRLFGK KEKARLGQLRGFMETEAAAQESLGLGKLGTQAEKDRRLKKKHELLEEARRKGLPFAQWDGPTVVAWLELW LGMPAWYVAACRANVKSGAIMSALSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPPTSRTPSGNV WVTHEEMENLTAPAKTKESEEGSWAQCPVFLQTLAYGDMNHEWIGNEWLPSLGLPQYRSYFMECLVDARM LDHLTKKDLRVHLKMVDSFHRTSLQYGIMCLKRLNYDRKELERRREASQHEIKDVLVWSNDRVIRWIQAI GLREYANNILESGVHGSLIALDENFDYSSLALLLQIPTQNTQARQILEREYNNLLALGTERRLDESDDKN FRRGSTWRRQFPPREVHGISMMPGSSETLPAGFRLTTTSGQSRKMTTDVASSRLQRLDNSTVRTYSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001205341
ORF Size	3771 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205341.1, NP_001192270.1
RefSeq Size	6454 bp
RefSeq ORF	3774 bp
Locus ID	327814
MW	143.3 kDa
Gene Summary	Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates (By similarity).[UniProtKB/Swiss-Prot Function]

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