Map4k4 (NM_001252200) Mouse Tagged ORF Clone
CAT#: MR231754
- TrueORF®
Map4k4 (myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 1
"NM_001252200" in other vectors (1)
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CNY 12,830.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 9430080K19Rik; AU043147; AU045934; AW046177; HGK; Nik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231754 representing NM_001252200
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACGACTCTCCCGCGAAGAGCCTGGTGGACATTGACCTGTCGTCCCTGCGGGACCCTGCTGGGA TTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAAC GGGTCAGCTGGCCGCCATCAAGGTTATGGACGTCACCGAGGATGAAGAGGAAGAAATCAAACTGGAGATA AATATGCTGAAGAAGTATTCTCATCATCGAAATATTGCCACGTACTATGGTGCTTTCATTAAGAAGAGCC CTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTTTGTGGGGCTGGGTCCATCACAGACCTTGT GAAGAACACCAAAGGGAACACTCTCAAAGAAGACTGGATTGCTTACATCTCCAGGGAAATCCTCAGGGGA TTGGCACATCTCCATATTCACCACGTTATTCACCGAGATATCAAGGGCCAAAATGTGCTGCTGACCGAGA ATGCTGAGGTGAAACTTGTTGATTTTGGTGTAAGCGCTCAGCTGGACAGGACGGTTGGACGGAGAAATAC GTTCATAGGCACACCCTACTGGATGGCTCCAGAGGTCATCGCCTGTGATGAGAACCCAGACGCCACTTAC GACTACAGAAGTGACCTCTGGTCCTGTGGCATCACAGCCATCGAGATGGCTGAAGGGGCCCCCCCTCTCT GTGACATGCATCCAATGAGAGCGCTGTTTCTCATCCCCAGAAACCCTCCTCCCAGGCTGAAGTCAAAAAA ATGGTCAAAGAAATTTTTCAGCTTTATAGAAGGCTGTCTGGTGAAGAATTACATGCAGCGGCCCTCTACA GAGCAACTTTTAAAACACCCTTTCATAAGGGATCAGCCCAATGAAAGGCAGGTTCGAATCCAGCTTAAGG ATCACATAGACCGGACCAGAAAGAAGAGAGGCGAGAAAGATGAGACGGAGTACGAGTACAGCGGGAGCGA GGAGGAGGAGGAGGAAGTGCCTGAGCAGGAGGGAGAGCCAAGTTCCATCGTCAATGTGCCTGGAGAGTCA ACTCTGCGACGTGATTTCCTGAGACTGCAGCAGGAGAACAAGGAGCGGTCTGAGGCTCTGCGGAGACAGC AGCTTCTGCAGGAGCAGCAGCTCCGGGAGCAGGAGGAGTATAAGAGGCAGCTGCTGGCTGAGAGGCAGAA GCGGATTGAACAGCAGAAAGAACAGAGGAGGCGGCTGGAAGAGCAACAAAGAAGAGAACGGGAAGCCAGG AGGCAGCAGGAGCGTGAGCAGCGGCGGCGTGAACAAGAGGAGAAGAGGCGTCTCGAGGAACTGGAAAGGC GGCGTAAAGAAGAGGAAGAGAGGAGACGGGCAGAAGAGGAGAAGAGGAGAGTGGAGAGGGAACAGGAGTA CATCAGGCGGCAGCTAGAGGAGGAGCAGCGGCACCTGGAGATCCTGCAGCAGCAGCTGCTCCAGGAGCAG GCCATGTTACTGCACGACCACAGGAGGCCGCACGCACAGCAGCAGCCGCCGCCCCCGCAGCAGCAGGACA GGAGCAAACCGAGCTTTCATGCTCCAGAGCCCAAGCCTCACTATGACCCTGCTGACAGAGCTCGGGAGGT GGAAGATAGATTTAGAAAGACTAACCACAGCTCCCCTGAGGCCCAGGCTAAGCAAACGGGCAGAGGACTG GAGCCACCCGTGCCTTCCCGGTCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCACCCTGCCCTGC AGAGACCAGCGGAGCCACAGGTACAGTGGTCCCACCTGGCATCTCTCAAGAACAATGTCTCCCCTGTCTC GAGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTCGCACACCACCATCTCCGCTCTCAGGACCCATGT CCACCTTCCCGCAGTGAGGGGCTCAGTCAGAGCTCTGACTCTAAGTCGGAGGTGCCCGAGCCCACCCAAA AGGCTTGGTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTTCCCGTGAGAACGACCTCTCGTTCCCC TGTCCTCTCCCGTCGGGATTCCCCACTGCAGGGCGGTGGACAGCAGAATAGCCAAGCAGGACAGAGAAAT TCCACCAGCAGTATTGAGCCCCGGCTTCTTTGGGAGAGAGTGGAAAAGCTGGTCCCCAGGCCAGGCAGTG GCAGCTCTTCAGGGTCCAGCAACTCAGGATCCCAGCCTGGCTCCCATCCTGGGTCTCAGAGCGGCTCCGG TGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGTTCTCCATCGCCACGCCAGGAAAGTGCAGCC AAAAAGCCTGACGATAAGAAAGAAGTATTCCGGCCTCTCAAGCCTGCGGGAGAAGTGGACTTGACTGCGT TGGCCAAAGAGCTTCGAGCAGTGGAAGATGTTCGGCCACCTCACAAAGTAACAGACTACTCATCCTCCAG TGAGGAGTCTGGGACCACAGATGAGGAGGAGGAGGACGTGGAACAGGAGGGGGCTGATGATTCTACCTCG GGACCAGAAGACACCAGAGCAGCGTCATCTCCGAACCTGAGCAACGGTGAAACAGAATCTGTGAAAACAA TGATCGTTCATGATGATGTAGAAAGTGAGCCAGCCATGACCCCGTCCAAGGAGGGCACCCTCATCGTCCG CCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTTTACACCTTTTATAGAC CCCAGGTTACTACAGATCTCTCCATCTAGTGGGACGACAGTGACTTCCGTGGTGGGATTTTCCTGTGATG GGCTGAGACCAGAAGCCATAAGGCAAGATCCTACTCGGAAAGGCTCAGTGGTCAATGTGAACCCCACGAA CACTAGGCCACAGAGTGATACCCCCGAGATTCGTAAATACAAGAAGAGATTTAACTCAGAGATCCTGTGT GCTGCCTTATGGGGAGTGAACTTGCTGGTTGGTACAGAGAGCGGTCTGATGCTCTTGGACAGAAGTGGCC AAGGGAAAGTATACCCTCTGATCAGCCGAAGACGGTTCCAGCAAATGGATGTGCTCGAAGGCCTAAATGT CCTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTATCCTGGCTAAGAAATAAGATT CTTCATAATGATCCAGAGGTTGAGAAGAAGCAGGGGTGGACCACTGTGGGCGACTTGGAAGGGTGCGTGC ACTATAAAGTCGTAAAATATGAAAGAATCAAGTTTCTGGTAATTGCTTTGAAGAGTTCTGTGGAAGTCTA TGCATGGGCACCGAAGCCATATCACAAATTTATGGCCTTTAAGTCATTTGGAGAACTGCTACATAAGCCA TTACTGGTGGATCTCACTGTGGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCGGGATTCC ATGCTGTTGATGTGGATTCAGGCTCAGTCTATGACATTTATCTACCAACACATATTCAGTGTAGCATCAA ACCCCATGCAATCATTATCCTCCCCAACACGGATGGGATGGAGCTGCTGGTGTGCTACGAAGACGAGGGG GTTTACGTTAACACGTACGGAAGGATCACCAAGGACGTGGTTCTGCAGTGGGGAGAGATGCCGACATCTG TAGCATATATCCGGTCGAATCAGACAATGGGCTGGGGAGAGAAGGCAATAGAGATACGATCTGTGGAAAC TGGTCACTTGGATGGTGTATTTATGCACAAAAGGGCTCAGAGACTAAAGTTCCTGTGTGAACGCAATGAC AAGGTGTTCTTTGCCTCCGTCCGCTCTGGTGGCAGCAGCCAGGTCTATTTCATGACCTTAGGCAGGACTT CTCTTCTGAGCTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231754 representing NM_001252200
Red=Cloning site Green=Tags(s) MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEI NMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRG LAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY DYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPST EQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGES TLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREAR RQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEILQQQLLQEQ AMLLHDHRRPHAQQQPPPPQQQDRSKPSFHAPEPKPHYDPADRAREVEDRFRKTNHSSPEAQAKQTGRGL EPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPC PPSRSEGLSQSSDSKSEVPEPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGGGQQNSQAGQRN STSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGSHPGSQSGSGERFRVRSSSKSEGSPSPRQESAA KKPDDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPHKVTDYSSSSEESGTTDEEEEDVEQEGADDSTS GPEDTRAASSPNLSNGETESVKTMIVHDDVESEPAMTPSKEGTLIVRQTQSASSTLQKHKSSSSFTPFID PRLLQISPSSGTTVTSVVGFSCDGLRPEAIRQDPTRKGSVVNVNPTNTRPQSDTPEIRKYKKRFNSEILC AALWGVNLLVGTESGLMLLDRSGQGKVYPLISRRRFQQMDVLEGLNVLVTISGKKDKLRVYYLSWLRNKI LHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFGELLHKP LLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYLPTHIQCSIKPHAIIILPNTDGMELLVCYEDEG VYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERND KVFFASVRSGGSSQVYFMTLGRTSLLSW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001252200 |
ORF Size | 3864 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001252200.1, NP_001239129.1 |
RefSeq Size | 5782 bp |
RefSeq ORF | 3867 bp |
Locus ID | 26921 |
UniProt ID | P97820 |
MW | 147.2 kDa |
Gene Summary | Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC229543 | Map4k4 (untagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4), transcript variant 1 |
CNY 12,350.00 |