Pcdh11x (NM_001271809) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PCDH; Pcdh11; PCDHX; PCDHX11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231772 representing NM_001271809 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGTTGTCCGGGACGTATATATTTGCGGTCCTGTTAGCATGCGTCGTGTTTCAGTCTGGCGCCC AGGAGAAGAATTATACAGTCCGAGAAGAAATGCCAGAAAACGTCCTGATAGGCGACTTGTTGAAAGACCT CAACTTGTCGCTTATTCCCGACAAGTCCTTAACCACTCCTATGCAGTTCAAATTAGTTTACAAAACCGGG GATGTACCATTGATTCGCATTGAAGAGGGTACTGGAGAGATCTTCACTACTGGTGCACGCATTGATCGTG AGAAATTATGTGCTGGAATCGTGTTGGATGCCCGTTGCTTTTACGAAGTGGAGGTTGCTGTTTTGCCGGA TGAAATATTTCGATTGGTTAAGATACGCTTTCTGATTGAAGACATAAATGATAATGCACCGTTATTCCCA ACAACTGTCATCAACATATCAATTCCGGAGAACTCGGCTATAAACTCTCGATATTCTCTTCCAGCAGCCA TTGATCCTGACATTGGAATAAATGGAGTCCAAAATTACCAACTAATTAAGAGTCAAAATATTTTCGGACT TGATGTCATTGAAACACCAGAAGGAGACAAGATGCCCCAACTGATTGTTCAAAAGGAGTTAGATAGAGAA GAGAAGGATACCTATGTGATGAAAGTAAAAGTTGAAGATGGTGGCTTTCCTCAAAGATCCAGTACAGCTA TTTTGCAAGTAAGTGTTGCTGATACAAACGACAATCACCCAATCTTCATAGAAAAGGAAATTGAAGTCAG TATACCAGAAAATGCTCCTATAGGTTCTTCAGTGACACAGCTCCATGCCACAGATGCAGATATAGGTGAG AATGCCAGAATTCACTTTTATTTCAGCAACCTAGTCTCCAACATCGCTAAGAGACTGTTTCATCTAAACA CCACTACTGGACTTATCACTGTCAAAGAACCACTGGATAGGGAAGAATCACCAAGTCACAAGTTATTGGT TTTGGCAACTGATGGTGGATCAACACCAGCAAGAGCAACGGTGCTGGTAAATGTCACAGATATCAATGAT AATGTCCCATCAATTGACATAAGATACATTGTCAATCCAACCAATGGCACTGTGCTTCTTTCAGAGAATG CTCCACTTAACACTAAAATTGCTCTCATAACTGTGATGGATAAGGATTCTGAACATAATGGTAGGGTGAC ATGCTTCACAGATCATGAAGTTCCTTTCAGATTGCGACCAGTATTCAGTAATCAGTTTCTCTTGGAGACT GCTGCATTTCTTGACTTTGAGTCCACAAGAGAATATGCCATAAAATTACTAGCCGCTGATGCTGGCAAAC CTCCTTTGAATCAGTCATCCATGCTCCTGATCAAAGTAAAAGATGAAAATGACAATGCTCCAGTTTTCAC CCAGTCTTTCATAAGCCTTTCTGTTCCTGAGAATAACTCTCCTGGTGCACAGTTGACAAAAATCAGTGCA ACAGATGCAGACAGTGGACAGAATGCTGAAATAAGCTACATGCTAGGTTTTGATGCACCACCTGAATTCA ATCTGGATCAACGTACTGGCATCCTGACTGCAGTGAAGAAACTAGATAGGGAAAAACAGGAAAAGTATTA CTTTACAGTTTTGGCACAGGACAATGGAATTCCACCCTTAATGTCCAATGCCACTGTATTTGTGACTGTT CTTGACCAGAATGATAATAGCCCAATTTTCACTCATAATGAATATAACTTCTATGTTCCTGAAAGCCTTC CAAAACATGGCACAGTAGGGCTAATAACTGTGACTGATCCTGATTACGGAGAGAATTCTGCAGTTACCCT CTCCATCTTAGATGTGAATGATCAATTCACTATTGATCCACAGTCTGGTGTCATCAGGCCAAATATTTCA TTTGACAGAGAAAGACAAGAATCCTATACTTTCTATGTAAAAGCTGAGGATGGTGGTAGGGTATCACGTT CTTCAACTGCTAGAGTAACCATAAATGTGGTTGATGTCAATGATAACAAACCAATTTTTATTGACCCTCC TTCCAATTACTCCTTTGAATGGGTTCTACCATCCACAAACCCTGGCACAGTTGTCTTCAAGGTTGTTGCA ATTGACGATGATATTGGCATGAATGCAGAGGTTCTTTACAGCATTGTTGGAGGAAATACAAAAGGACTGT TTATGATTGAACAAACATCAGGTAACATCACATTGAAGGAGAAGTGCATGGTTTCAGATCTTGGTTTACA CCGAGTCATAGTCAAAGCTAATGATTTAGGACAACCTGATTCTCTCTTCAATGTTGTAAATGTCAATTTC TTTATAAATGAGTCCGTGCCCAATGCCACACTGATTTATGAACTGGTGCGCAGAAGCATTGATGCACCTG CCAATCAAAATACTGAAACAACCAGTGCATCCTCACCAACCACTGACTATGTCAAGATCATGGTTGCCAT TGTGGCTGGCACCATAACTGTTGTCCTAGTTATTTTCATCACTGCTGTAGTAAGATGCCGCCAACCACCA CATCTTAAGGCTTCTCAGAAAAACAAACAGAATTCTGAGTGGGTTACTCCAAACCCAGAAAACAGGCAGA TGATTATGATGAAGAAGAAGAAGAAAAAGAAGAAGAAGCATCCCCCCAAGAACTTGCTGCTTAATTTTGT CACTATTGAAGAAGCAAAGCCAGATGACGGTGAAAATGAGAGAAACAGTGTCACACTAGATCTTCCAATT GAGCTGGAAGAGCAAACCATGGGCAAATACAACTGGGGCACTACACCTACTACTTTCAAACCTGATAGCC CTGATTTGGCTCGACACTACAAATCGGCCTCTCCTCAGCCTGCATTCCAGATCCAGCCTGAAACGCCCCT GAACTCAAAGCACCACATCATTCAGGAACTGCCTCTTGATAATACCTTCGTTGGCTGTGATTCCATCTCC AAGTGCTCCTCCAGCAGTTCTGATCCCTACAGTGTTTCTGAGTGTAGCTATCCAGTGACAACTTTCAAGG CCCCTGTGTCTGTGCATATCAGACCGACAATGAAGGAGGTGGTAAGATCTCACACACCCATGAAAGAGGC AACCACTGTGGAAATCTGGACTCATCCACATCCACAGCGGCGATCTGATGGGAAAAAAGCAGGAAAGTCC CAGAGACGTGTCACATTTCACCTACCAGAAGGCTCTCAGGAAAGCATCAGTGATGGTGGATTGGGAGACC ACGATGCAGGCAGCCTTCCCAGTACATCCCATGCACTGCCTCTTGGCTATCCTCAGGAAGAGTATTTTGA TCATGCTGCACCAAACAACCGCACTGAAGGGGATGGCAACTCTGATCCTGAATCTACTGCAGAAATAACA GTGCAGCCAACTGTGGAAGAAGCCTCTGATACCTGTACTCAAGAATGCCTCATCTTGGGACACTCTGACT CCTGCTGGATGCCAGCTACTTTGACCAATCCTAGCCCTTCACAGATAAAGACCTCTGCTATCTGCCACAG CCCACCCAGGCCCCGCTTGTCAGTTCGTCGCTACAGCCCTCCAGTGACACAGACGGTCACTATTTGCCAC AGCCCCCCTGTGACCCAAGCTATTGCACTGTGTCACAGCCCTCCACCAGTACAGGTTACTGTACCCCGCC ATAGTCCACCACCAGCACAGGCCTCTGCAGTCAGCTACAGCCCAACTCTAGTACAGGCTGTGGTAATTCA CCACAGTCCTCCTCTGCCACAGGCTGCCACACACCATCGCACTCAGGCACAACCACCAATGGGTTTGCAG CAGGGATGGGTGCAGGGTGCTGGAGCAGATGGACTGTATCCTATTGATCAGGGAGTACAGGGCAGTACAA GAGCTCAGTTTTACACCATGGCTGAAAGATTTCATCCCGATGATGACTCAATTAAAGTCATTCCCTTGAC CACCTTTACTTCAGGTCAACAGGCCAGATCCTCAAGAGGTGATTCTCCAATCATAGAGGAACACCCCTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231772 representing NM_001271809 Red=Cloning site Green=Tags(s) MDLLSGTYIFAVLLACVVFQSGAQEKNYTVREEMPENVLIGDLLKDLNLSLIPDKSLTTPMQFKLVYKTG DVPLIRIEEGTGEIFTTGARIDREKLCAGIVLDARCFYEVEVAVLPDEIFRLVKIRFLIEDINDNAPLFP TTVINISIPENSAINSRYSLPAAIDPDIGINGVQNYQLIKSQNIFGLDVIETPEGDKMPQLIVQKELDRE EKDTYVMKVKVEDGGFPQRSSTAILQVSVADTNDNHPIFIEKEIEVSIPENAPIGSSVTQLHATDADIGE NARIHFYFSNLVSNIAKRLFHLNTTTGLITVKEPLDREESPSHKLLVLATDGGSTPARATVLVNVTDIND NVPSIDIRYIVNPTNGTVLLSENAPLNTKIALITVMDKDSEHNGRVTCFTDHEVPFRLRPVFSNQFLLET AAFLDFESTREYAIKLLAADAGKPPLNQSSMLLIKVKDENDNAPVFTQSFISLSVPENNSPGAQLTKISA TDADSGQNAEISYMLGFDAPPEFNLDQRTGILTAVKKLDREKQEKYYFTVLAQDNGIPPLMSNATVFVTV LDQNDNSPIFTHNEYNFYVPESLPKHGTVGLITVTDPDYGENSAVTLSILDVNDQFTIDPQSGVIRPNIS FDRERQESYTFYVKAEDGGRVSRSSTARVTINVVDVNDNKPIFIDPPSNYSFEWVLPSTNPGTVVFKVVA IDDDIGMNAEVLYSIVGGNTKGLFMIEQTSGNITLKEKCMVSDLGLHRVIVKANDLGQPDSLFNVVNVNF FINESVPNATLIYELVRRSIDAPANQNTETTSASSPTTDYVKIMVAIVAGTITVVLVIFITAVVRCRQPP HLKASQKNKQNSEWVTPNPENRQMIMMKKKKKKKKKHPPKNLLLNFVTIEEAKPDDGENERNSVTLDLPI ELEEQTMGKYNWGTTPTTFKPDSPDLARHYKSASPQPAFQIQPETPLNSKHHIIQELPLDNTFVGCDSIS KCSSSSSDPYSVSECSYPVTTFKAPVSVHIRPTMKEVVRSHTPMKEATTVEIWTHPHPQRRSDGKKAGKS QRRVTFHLPEGSQESISDGGLGDHDAGSLPSTSHALPLGYPQEEYFDHAAPNNRTEGDGNSDPESTAEIT VQPTVEEASDTCTQECLILGHSDSCWMPATLTNPSPSQIKTSAICHSPPRPRLSVRRYSPPVTQTVTICH SPPVTQAIALCHSPPPVQVTVPRHSPPPAQASAVSYSPTLVQAVVIHHSPPLPQAATHHRTQAQPPMGLQ QGWVQGAGADGLYPIDQGVQGSTRAQFYTMAERFHPDDDSIKVIPLTTFTSGQQARSSRGDSPIIEEHPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001271809
ORF Size	3990 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271809.1, NP_001258738.1
RefSeq Size	8637 bp
RefSeq ORF	3993 bp
Locus ID	245578
MW	146.9 kDa
Gene Summary	This gene encodes a member of the protocadherin family, and cadherin superfamily, of transmembrane proteins containing cadherin domains. The encoded protein may mediate cell-cell adhesion in neuronal tissues in the presence of calcium. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Nov 2012]

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